topbp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45147 XM_018094989.1
Latest version!
Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa60131 XM_002937745.4
Latest version!
Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa60131 XM_004915368.4
Latest version!
Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa60132 XM_004915369.3
Latest version!
Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa11878 XM_002937745.3
Latest version!
Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa11878 XM_004915368.3
Latest version!
Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa11879 XM_004915369.2
Latest version!
Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45147
    Clone ID Related Accession (Same CDS sequence) XM_018094989.1
    Accession Version XM_018094989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAAGAAT ATTTGTGCCC AATATTTCGT GGTTGCACTA TCTGTGTCAC AGGATTAAAC 
    AGTTTAGACA GAAAGGAGGT GCAACGTCTT ACAACCCTCC ATGGAGGAGA GTACACAGGG
    CAACTCAAAA TGAATGAATC TACACACCTT ATTGTACAAG AGGCTAAAGG CCAGAAATAT
    GAGTGTGCCA GAAAGTGGAA TGTACACTGT GTCTCCGTTC AATGGTTTTT CGACAGTCTG
    GACAAAGGAT TCTGTCAGGA TGAAACAATG TATAAAATTG AGCCTGCTAC TAGTTTAAAA
    TCTGTGCCAG ATACATCCAC TCCTACAGGT GACAATAAGA AACCAGATAG TCGGGCCATT
    TATGATGTCA GCCAAATTTC AAATATAAGT ACAAGCTGCG TTAACGAATC GGCTTTTAAT
    TCTGTGATGA CCAGCAGACT GGATCCTCCA GCAGATGCCC TTGAGAACTT GGATATCAGT
    TCATTACAAG CTCCAGAAGA CCTTCTTGAT GGCTGCCGAA TATATTTGTG CGGGTTTGGG
    GGAAGAAAGT TGGACAAGTT GAGAAGGCTA ATCAACAGTG GTGGGGGTGT GCGATTTAGT
    CAGCTTACCG CAGATGTAAC CCATATTATT GTTGGAGAAA CTGATGAAGA ACTAAAGCAG
    TTTTTGAGCA AAACTCAACA CAGGCCTTAT GTGTTAACTG TAAAATGGCT ACTGGAAAGC
    TTTTCAAAAG GACATCTGCA ACCTGAGAAC ATATATTCCC ATTCAAGCTA TCAACAAACT
    GAGCTTTCTT CACCAGTTCA GCCTGCCTTT AATTTAACTG CTCAAAAGAT CAGTAGTACA
    CGAGGGCCTC TAAACCACAC TCACAACCAA CAGGCAGATG AGGACCTGCT GTCTCAGTAC
    ACAGAAAATA ACTCCACTTT AATTGAGGAC GAACATCCTA AAATCTCTAA TACCAACAGT
    ATTTCTCAAA TGAGCATGCA TGAAGATATG ACATCTTGTA CCAGCCAAAG TGGTTTGGCG
    GATACATCCA CCATAATTGA AGGAGGCTTA TTCAGCCGTA AACAATTCTT GGTGCTGGGC
    TTTTTGGAGG AAGATGAGGC ATGCATCATA GACATAATAA AAAAGAATGC TGGCAAGGTG
    CTTTCATCTC AGAAAAGAGC CATTGCTGAT TATGCTGTAG TTCCCTTACT GGGTTGTGAG
    GTGGAATCTA CAGTTGGAGA GGTTGTGACC AATGCTTGGC TGGGCATGTG TATAGAACAA
    GAGAAGCTCC TAGACCCACA TTCAAATGCA CTTTTTACAC CTGTGCCATG TTTGGAAGGT
    AGTCGACCGC TGCAAGAGTG CGTGCTTTCT GTCAGCCAGT TTATGGGCGC TGAAAGGGAT
    TCATTAGTTT ACTTGGCCGG TTTGCTTGGA GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG
    GCCAATCCAA AAAAGGACAT GTTTGCCAGT ACACACTTAG TTCTTAAAGA TGCTGAAGGG
    TCAAAATACG AAGCAGCCAA AAAGTGGAAT TTGCCAGCAG TGACAATGAA GTGGCTTTTG
    CAGTGTGCTA GAACTGGTAA AAAAGCAGAT GAACACCATT ATCTTGTTGA CAGTGTTCCT
    GAAGAAGATG AGAGTTTTAT AAGTCAGACA TACAAACCAC AAGTATCCAG GCGATCAATG
    GATGCACCAT GCCATCAAGA AAACCATCCA CAAGCCCTAA AGAAAGCTGC AGTTACCCCA
    CTTGACATGA ACCGGTTTCA GAGCAAAGCA TTTCAGTCTG TTATTTCTCA GCATAACAAG
    AACCCACAAA CACCTGGAGG GGAAAGCACA GTTCTTCAGA GAGAACCATC TTTGCATCTT
    GACACACCAT CAAAATTTCT TTCCAAAGAC AAACTTTTCA AACCCTCCTT TGATGTTAAG
    GATGCTCTTG CAGCTCTGGA AACACCCGGA GGTCCCAACC AAAAAAGCAG GACACAGAGC
    ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT TTGCAGCTGG CTATTGCAAA CAGCACACGT
    CAAACTGCTG CGGTCACTGC AAGCCCTCAG CTAAAGGCAG CAGAACAAAA AGAGCAGTTA
    GAGAACTCCA AGGTACTGCA TAATGTTGTT ATATGTGTGA GCAAGAAGCT AATTAAAAAA
    CAAGGTGAAC TGAATGGCAT TGCTGCTTCA CTTGGAGCAG AATACAGATG GTGCTTCGAT
    GAAAGCGTGA CCCATTTTAT CTACCACGGA CGACAAAACG ACATGAGTCG AGAATACAAA
    TCTGTGAAAG AACGAAGTGG CATCTGCATA GTTTCTGAAC ACTGGCTATT AGCTTGTTCA
    GAACAACAGA AGAGAGTACC TGAGGCTCTC TATCCCCATA CCTACAATCC TAAAATGAGC
    TTGGATATCA GTGGTGTACA AGATGGATCT CACACAGCCA GCAAATTCTC AGCAGACACC
    TCTCTTCAGC TGGATGAGAA TTGTGAATTA AGGCTTCAGC AGAATGATGA ACTTGGGGAG
    ACATCCGATG ACCAGGTTAA AAAGGAAGCT GATAATAGAG ATCCTCAAAA CCCTTTAAAA
    GAAGTGAAAG GAGCTTTAAC TCAGACTTTA GAGATGAGAG AAAATTTCCA GCGGCAGCTA
    CAAGAATTAA TGTCTGCAAC TTCAGTGGTA AAGCCTCAAG GGCCCCGGGG CTCCTTGACT
    AGAGTTGGCT TTGATAATTC TCCTTGTACA CCTGAAGGGG CACGTTCTAC ACGTAATGGA
    AGAAGTAGAG TTTTGGAAGC ACTAAGACAG TCTCGGCAGG CTACAACAGA CCTCAATACA
    GAGCCATCAC AGAACGAGCA AATAATTTGG GATGATCCTA CTGCCAGAGA AGAAAGAGCA
    AAGCTGGTCA GCAATCTACA GTGGCCCGAC AGTCCCTCCC AGTATTCAGA ACAGCTTCAG
    CTTAATATGA CTGATGCTGG TGGAAATTAT GCAGCAGTAA AGGAATCTTT AACAGATACT
    GAAATAGCAG AATTGGAAGC TTGTGAATTT GAGCATAAAT CATTCACTAG AGCTCCTATG
    ACAGAGAATG ATTTGCAGTC TCCAAACAAA CTGGATCAAC TCACTCCTAC CCCACAAGCT
    CCAAGCATTG CATTTCCACT TGCCAACCCT CCAGTGGCAC CACAACCTAA AGAAAAGCCT
    GTGAAACCAT TTTCAGAGGA GAAAACCATA AAGGAGCGTC GCTTCCAGCT ATCATCCTTG
    GACCCTCAAG AACGAATTGA TTACTCACAG CTTATTGAGG AAATAGGGGG GGTGGTGATA
    GAAAAACAGT GTTTTGATCC AAGCTGCACA CATATCATTG TGGGGCATCC TCTTCGGAAT
    GAGAAATATT TGGCCTCAAT GGCTGCAGGA AAGTGGGTAC TACACCGGTC ATATCTGGAA
    GCCTGCAGAG CTGCAAAACA ATTCATACAG GAGGATGACT ATGAATGGGG AAGCAAGTCT
    ATACTGAGTG CTGTGACCAA CATAAATCCC CAGCAGAGGA TGCTGGCAGA GGCTGCAATG
    AGATGGAGGA AAAAGCTGCA AGGAATAAAG CAAAATATGG GCATTGCTGA GGGTGCATTC
    AGCGGCTGGA AAGTCATTTT AAATGTTGAC CAAACAAAGG AACCTGGTTT CAAACGTTTG
    CTTCAGTCAG GAGGTGCAAA GGTATTTGCT GGCCATTCTT CTGCTCTGTT TAAAGAAGCC
    AACCACCTCT TTGCTGACTT CAGCAAACTG AAACCTGATG AGCCCAAGGT GAATGTGGCC
    GAGGCTGCAG CACAAGGAGT AAACTGCCTA AAGCCAGAGT ATATTGCCGA CTACCTCATG
    AAGGAGCCTC CTCCACCAGT CAACAATTAC TGCCTCCCTG ATGCTCTGCC ATATCTTCAG
    ATCACAGTGA CTGGGCTTTC TCGCAAAAGG AAAAACTCTG GAGACATCTC TGATGTTAAG
    AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950478.1
    CDS110..4087
    Translation

    Target ORF information:

    RefSeq Version XM_018094989.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAAGAAT ATTTGTGCCC AATATTTCGT GGTTGCACTA TCTGTGTCAC AGGATTAAAC 
    AGTTTAGACA GAAAGGAGGT GCAACGTCTT ACAACCCTCC ATGGAGGAGA GTACACAGGG
    CAACTCAAAA TGAATGAATC TACACACCTT ATTGTACAAG AGGCTAAAGG CCAGAAATAT
    GAGTGTGCCA GAAAGTGGAA TGTACACTGT GTCTCCGTTC AATGGTTTTT CGACAGTCTG
    GACAAAGGAT TCTGTCAGGA TGAAACAATG TATAAAATTG AGCCTGCTAC TAGTTTAAAA
    TCTGTGCCAG ATACATCCAC TCCTACAGGT GACAATAAGA AACCAGATAG TCGGGCCATT
    TATGATGTCA GCCAAATTTC AAATATAAGT ACAAGCTGCG TTAACGAATC GGCTTTTAAT
    TCTGTGATGA CCAGCAGACT GGATCCTCCA GCAGATGCCC TTGAGAACTT GGATATCAGT
    TCATTACAAG CTCCAGAAGA CCTTCTTGAT GGCTGCCGAA TATATTTGTG CGGGTTTGGG
    GGAAGAAAGT TGGACAAGTT GAGAAGGCTA ATCAACAGTG GTGGGGGTGT GCGATTTAGT
    CAGCTTACCG CAGATGTAAC CCATATTATT GTTGGAGAAA CTGATGAAGA ACTAAAGCAG
    TTTTTGAGCA AAACTCAACA CAGGCCTTAT GTGTTAACTG TAAAATGGCT ACTGGAAAGC
    TTTTCAAAAG GACATCTGCA ACCTGAGAAC ATATATTCCC ATTCAAGCTA TCAACAAACT
    GAGCTTTCTT CACCAGTTCA GCCTGCCTTT AATTTAACTG CTCAAAAGAT CAGTAGTACA
    CGAGGGCCTC TAAACCACAC TCACAACCAA CAGGCAGATG AGGACCTGCT GTCTCAGTAC
    ACAGAAAATA ACTCCACTTT AATTGAGGAC GAACATCCTA AAATCTCTAA TACCAACAGT
    ATTTCTCAAA TGAGCATGCA TGAAGATATG ACATCTTGTA CCAGCCAAAG TGGTTTGGCG
    GATACATCCA CCATAATTGA AGGAGGCTTA TTCAGCCGTA AACAATTCTT GGTGCTGGGC
    TTTTTGGAGG AAGATGAGGC ATGCATCATA GACATAATAA AAAAGAATGC TGGCAAGGTG
    CTTTCATCTC AGAAAAGAGC CATTGCTGAT TATGCTGTAG TTCCCTTACT GGGTTGTGAG
    GTGGAATCTA CAGTTGGAGA GGTTGTGACC AATGCTTGGC TGGGCATGTG TATAGAACAA
    GAGAAGCTCC TAGACCCACA TTCAAATGCA CTTTTTACAC CTGTGCCATG TTTGGAAGGT
    AGTCGACCGC TGCAAGAGTG CGTGCTTTCT GTCAGCCAGT TTATGGGCGC TGAAAGGGAT
    TCATTAGTTT ACTTGGCCGG TTTGCTTGGA GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG
    GCCAATCCAA AAAAGGACAT GTTTGCCAGT ACACACTTAG TTCTTAAAGA TGCTGAAGGG
    TCAAAATACG AAGCAGCCAA AAAGTGGAAT TTGCCAGCAG TGACAATGAA GTGGCTTTTG
    CAGTGTGCTA GAACTGGTAA AAAAGCAGAT GAACACCATT ATCTTGTTGA CAGTGTTCCT
    GAAGAAGATG AGAGTTTTAT AAGTCAGACA TACAAACCAC AAGTATCCAG GCGATCAATG
    GATGCACCAT GCCATCAAGA AAACCATCCA CAAGCCCTAA AGAAAGCTGC AGTTACCCCA
    CTTGACATGA ACCGGTTTCA GAGCAAAGCA TTTCAGTCTG TTATTTCTCA GCATAACAAG
    AACCCACAAA CACCTGGAGG GGAAAGCACA GTTCTTCAGA GAGAACCATC TTTGCATCTT
    GACACACCAT CAAAATTTCT TTCCAAAGAC AAACTTTTCA AACCCTCCTT TGATGTTAAG
    GATGCTCTTG CAGCTCTGGA AACACCCGGA GGTCCCAACC AAAAAAGCAG GACACAGAGC
    ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT TTGCAGCTGG CTATTGCAAA CAGCACACGT
    CAAACTGCTG CGGTCACTGC AAGCCCTCAG CTAAAGGCAG CAGAACAAAA AGAGCAGTTA
    GAGAACTCCA AGGTACTGCA TAATGTTGTT ATATGTGTGA GCAAGAAGCT AATTAAAAAA
    CAAGGTGAAC TGAATGGCAT TGCTGCTTCA CTTGGAGCAG AATACAGATG GTGCTTCGAT
    GAAAGCGTGA CCCATTTTAT CTACCACGGA CGACAAAACG ACATGAGTCG AGAATACAAA
    TCTGTGAAAG AACGAAGTGG CATCTGCATA GTTTCTGAAC ACTGGCTATT AGCTTGTTCA
    GAACAACAGA AGAGAGTACC TGAGGCTCTC TATCCCCATA CCTACAATCC TAAAATGAGC
    TTGGATATCA GTGGTGTACA AGATGGATCT CACACAGCCA GCAAATTCTC AGCAGACACC
    TCTCTTCAGC TGGATGAGAA TTGTGAATTA AGGCTTCAGC AGAATGATGA ACTTGGGGAG
    ACATCCGATG ACCAGGTTAA AAAGGAAGCT GATAATAGAG ATCCTCAAAA CCCTTTAAAA
    GAAGTGAAAG GAGCTTTAAC TCAGACTTTA GAGATGAGAG AAAATTTCCA GCGGCAGCTA
    CAAGAATTAA TGTCTGCAAC TTCAGTGGTA AAGCCTCAAG GGCCCCGGGG CTCCTTGACT
    AGAGTTGGCT TTGATAATTC TCCTTGTACA CCTGAAGGGG CACGTTCTAC ACGTAATGGA
    AGAAGTAGAG TTTTGGAAGC ACTAAGACAG TCTCGGCAGG CTACAACAGA CCTCAATACA
    GAGCCATCAC AGAACGAGCA AATAATTTGG GATGATCCTA CTGCCAGAGA AGAAAGAGCA
    AAGCTGGTCA GCAATCTACA GTGGCCCGAC AGTCCCTCCC AGTATTCAGA ACAGCTTCAG
    CTTAATATGA CTGATGCTGG TGGAAATTAT GCAGCAGTAA AGGAATCTTT AACAGATACT
    GAAATAGCAG AATTGGAAGC TTGTGAATTT GAGCATAAAT CATTCACTAG AGCTCCTATG
    ACAGAGAATG ATTTGCAGTC TCCAAACAAA CTGGATCAAC TCACTCCTAC CCCACAAGCT
    CCAAGCATTG CATTTCCACT TGCCAACCCT CCAGTGGCAC CACAACCTAA AGAAAAGCCT
    GTGAAACCAT TTTCAGAGGA GAAAACCATA AAGGAGCGTC GCTTCCAGCT ATCATCCTTG
    GACCCTCAAG AACGAATTGA TTACTCACAG CTTATTGAGG AAATAGGGGG GGTGGTGATA
    GAAAAACAGT GTTTTGATCC AAGCTGCACA CATATCATTG TGGGGCATCC TCTTCGGAAT
    GAGAAATATT TGGCCTCAAT GGCTGCAGGA AAGTGGGTAC TACACCGGTC ATATCTGGAA
    GCCTGCAGAG CTGCAAAACA ATTCATACAG GAGGATGACT ATGAATGGGG AAGCAAGTCT
    ATACTGAGTG CTGTGACCAA CATAAATCCC CAGCAGAGGA TGCTGGCAGA GGCTGCAATG
    AGATGGAGGA AAAAGCTGCA AGGAATAAAG CAAAATATGG GCATTGCTGA GGGTGCATTC
    AGCGGCTGGA AAGTCATTTT AAATGTTGAC CAAACAAAGG AACCTGGTTT CAAACGTTTG
    CTTCAGTCAG GAGGTGCAAA GGTATTTGCT GGCCATTCTT CTGCTCTGTT TAAAGAAGCC
    AACCACCTCT TTGCTGACTT CAGCAAACTG AAACCTGATG AGCCCAAGGT GAATGTGGCC
    GAGGCTGCAG CACAAGGAGT AAACTGCCTA AAGCCAGAGT ATATTGCCGA CTACCTCATG
    AAGGAGCCTC CTCCACCAGT CAACAATTAC TGCCTCCCTG ATGCTCTGCC ATATCTTCAG
    ATCACAGTGA CTGGGCTTTC TCGCAAAAGG AAAAACTCTG GAGACATCTC TGATGTTAAG
    AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60131
    Clone ID Related Accession (Same CDS sequence) XM_002937745.4 , XM_004915368.4
    Accession Version XM_002937745.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937745.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937791.3
    CDS138..4685
    Translation

    Target ORF information:

    RefSeq Version XM_002937745.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937745.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60131
    Clone ID Related Accession (Same CDS sequence) XM_002937745.4 , XM_004915368.4
    Accession Version XM_004915368.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915368.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915425.2
    CDS246..4793
    Translation

    Target ORF information:

    RefSeq Version XM_004915368.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915368.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60132
    Clone ID Related Accession (Same CDS sequence) XM_004915369.3
    Accession Version XM_004915369.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA
    TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT
    GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA
    CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT
    TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT
    CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC
    ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG
    CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT
    AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG
    ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG
    CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT
    GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT
    CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT
    GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT
    CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA
    GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG
    CATAAATCAG TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG
    GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA
    GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG
    GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT
    ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT
    ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG
    TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG
    GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG
    CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA
    AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA
    ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC
    CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA
    CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG
    CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC
    CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA
    AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915426.2
    CDS133..4677
    Translation

    Target ORF information:

    RefSeq Version XM_004915369.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915369.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA
    TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT
    GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA
    CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT
    TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT
    CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC
    ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG
    CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT
    AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG
    ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG
    CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT
    GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT
    CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT
    GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT
    CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA
    GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG
    CATAAATCAG TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG
    GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA
    GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG
    GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT
    ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT
    ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG
    TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG
    GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG
    CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA
    AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA
    ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC
    CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA
    CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG
    CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC
    CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA
    AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11878
    Clone ID Related Accession (Same CDS sequence) XM_002937745.3 , XM_004915368.3
    Accession Version XM_002937745.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937791.2
    CDS140..4687
    Translation

    Target ORF information:

    RefSeq Version XM_002937745.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11878
    Clone ID Related Accession (Same CDS sequence) XM_002937745.3 , XM_004915368.3
    Accession Version XM_004915368.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915425.1
    CDS98..4645
    Translation

    Target ORF information:

    RefSeq Version XM_004915368.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915368.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT
    ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA
    CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA
    CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA
    GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC
    TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT
    CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA
    AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT
    GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA
    GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA
    AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA
    CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG
    TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG
    GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC
    AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT
    GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT
    GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA
    CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT
    CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA
    AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG
    CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA
    CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA
    AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG
    GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC
    CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG
    CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC
    CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT
    GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG
    AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA
    AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC
    TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG
    AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11879
    Clone ID Related Accession (Same CDS sequence) XM_004915369.2
    Accession Version XM_004915369.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004915369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA
    TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT
    GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA
    CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT
    TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT
    CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC
    ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG
    CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT
    AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG
    ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG
    CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT
    GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT
    CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT
    GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT
    CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA
    GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG
    CATAAATCAT TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG
    GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA
    GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG
    GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT
    ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT
    ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG
    TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG
    GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG
    CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA
    AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA
    ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC
    CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA
    CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG
    CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC
    CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA
    AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915426.1
    CDS140..4684
    Translation

    Target ORF information:

    RefSeq Version XM_004915369.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
    GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG
    CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT
    GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG
    CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT
    GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT
    AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT
    GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA
    AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT
    CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT
    GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT
    ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT
    ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT
    GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG
    TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT
    GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT
    ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA
    GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT
    GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA
    ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT
    GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT
    GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC
    ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT
    AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA
    CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC
    GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG
    ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA
    TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA
    GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT
    TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC
    AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA
    CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT
    GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA
    GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT
    ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT
    TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT
    GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA
    TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA
    CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA
    TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA
    GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC
    AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA
    GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT
    TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG
    CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA
    TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT
    GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA
    CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT
    TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT
    CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC
    ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG
    CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT
    AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG
    ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG
    CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT
    GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT
    CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT
    GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT
    CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA
    GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG
    CATAAATCAT TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG
    GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA
    GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG
    GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT
    ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT
    ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG
    TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG
    GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG
    CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA
    AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA
    ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC
    CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA
    CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG
    CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC
    CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA
    AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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