uhrf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Gene Symbol	uhrf1
Entrez Gene ID	100491405
Full Name	ubiquitin-like with PHD and ring finger domains 1
General protein information	Preferred Names ubiquitin-like with PHD and ring finger domains 1 Names E3 ubiquitin-protein ligase UHRF1 Ubiquitin-like PHD and RING finger domain-containing protein 1 Ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012963705.2	XP_012819159.1	E3 ubiquitin-protein ligase UHRF1
XM_012963705.3	XP_012819159.1	E3 ubiquitin-protein ligase UHRF1
XM_002940137.4	XP_002940183.2	E3 ubiquitin-protein ligase UHRF1
XM_002940137.5	XP_002940183.2	E3 ubiquitin-protein ligase UHRF1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UHRF1	XP_001139916.2
Rattus norvegicus (Norway rat)	Uhrf1	NP_001008882.1
	UHRF1	XP_001083134.2
Bos taurus (cattle)	UHRF1	NP_001096568.1
Gallus gallus (chicken)	UHRF1	XP_418269.4
Danio rerio (zebrafish)	uhrf1	XP_003201004.1
Xenopus tropicalis (tropical clawed frog)	uhrf1	XP_004914675.1
Mus musculus (house mouse)	Uhrf1	NP_035061.3
Homo sapiens (human)	UHRF1	NP_037414.3
Canis lupus familiaris (dog)	UHRF1	XP_005633077.1

Gene Ontology

The following uhrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uhrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10593	XM_012963705.2 Latest version!	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa10593	XM_002940137.4 Latest version!	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa48333	XM_002940137.5 Latest version!	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa48333	XM_012963705.3 Latest version!	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uhrf1 ( XM_012963705.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819159.1 Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10593
Clone ID Related Accession (Same CDS sequence)	XM_012963705.2 , XM_002940137.4
Accession Version	XM_012963705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UHRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC CGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819159.1
CDS	108..2432
Translation	MWIQVRTMDGRDTRRIDSLSKLTKVEDLRARIQQIFGVALESQRLFYRGKQMENGHTLFDYSVGLNDIVQLLVRQIPDSVPTKDKECGISDADSGCGSGQGESDKNSSCGEGATDVDGQPAGINSENVGPSLYKKNDLVDARDLNMGAWFEAQIVSVSKRVNPDGMSAEILDTSAASDDIIYHVKYEDYPENGVVQLTYKDVRLRARTTLPWHDLKVGQVVMVNYNPDEPKERGYWYDAEILRKRETRTIKEIYVKVLLGDAGDSLNDCRIRFVDEIYKIEEPGSAYITTESPQKRQNGPECKHCKDNPKRACRMCACYVCGGKQDPEKQLLCDECDMAFHIYCLKPPLSAIPQDEDWYCPDCRNDASEVVLAGEKLKESKKKAKMASASSSSQRDWGKGMACVGRSRECTIVPSNHYGPIPGVPVGTLWKFRVQVSESGVHRPHVAGIHGRSNDGSYSLVLAGGYEDDVDNGSEFTYTGSGGRDLSGNKRTAEQSCDQKLTNMNRALALNCSAPINDKEGAVAKDWRAGKPVRVVRNTKGKKHSKYAPEDGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDEEPAPWSKEGKERIKKLGLVMQYPDGYLESLASKEREKENKTEDELSESPSKGKRKRNSGSGLSDAKSTPKKTKVESYKLSLDQKTLIKQDDLNAKLWREVMSFLKEGPKFLSKVEETFLCICCQEVVYEPITTECHHNICKGCLDRSFKALVHNCPACRHDLGKNYSLNVNKPLQAILSQLFPGYERGR

Target ORF information:

RefSeq Version	XM_012963705.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963705.2

ORF Insert Sequence:

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC CGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uhrf1 ( XM_002940137.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940183.2 Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10593
Clone ID Related Accession (Same CDS sequence)	XM_012963705.2 , XM_002940137.4
Accession Version	XM_002940137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UHRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940137.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC CGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940183.2
CDS	177..2501
Translation	MWIQVRTMDGRDTRRIDSLSKLTKVEDLRARIQQIFGVALESQRLFYRGKQMENGHTLFDYSVGLNDIVQLLVRQIPDSVPTKDKECGISDADSGCGSGQGESDKNSSCGEGATDVDGQPAGINSENVGPSLYKKNDLVDARDLNMGAWFEAQIVSVSKRVNPDGMSAEILDTSAASDDIIYHVKYEDYPENGVVQLTYKDVRLRARTTLPWHDLKVGQVVMVNYNPDEPKERGYWYDAEILRKRETRTIKEIYVKVLLGDAGDSLNDCRIRFVDEIYKIEEPGSAYITTESPQKRQNGPECKHCKDNPKRACRMCACYVCGGKQDPEKQLLCDECDMAFHIYCLKPPLSAIPQDEDWYCPDCRNDASEVVLAGEKLKESKKKAKMASASSSSQRDWGKGMACVGRSRECTIVPSNHYGPIPGVPVGTLWKFRVQVSESGVHRPHVAGIHGRSNDGSYSLVLAGGYEDDVDNGSEFTYTGSGGRDLSGNKRTAEQSCDQKLTNMNRALALNCSAPINDKEGAVAKDWRAGKPVRVVRNTKGKKHSKYAPEDGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDEEPAPWSKEGKERIKKLGLVMQYPDGYLESLASKEREKENKTEDELSESPSKGKRKRNSGSGLSDAKSTPKKTKVESYKLSLDQKTLIKQDDLNAKLWREVMSFLKEGPKFLSKVEETFLCICCQEVVYEPITTECHHNICKGCLDRSFKALVHNCPACRHDLGKNYSLNVNKPLQAILSQLFPGYERGR

Target ORF information:

RefSeq Version	XM_002940137.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940137.4

ORF Insert Sequence:

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC CGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uhrf1 ( XM_012963705.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819159.1 Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.

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CloneID	OXa48333
Clone ID Related Accession (Same CDS sequence)	XM_012963705.3 , XM_002940137.5
Accession Version	XM_012963705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UHRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC TGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819159.1
CDS	121..2445
Translation	MWIQVRTMDGRDTRRIDSLSKLTKVEDLRARIQQIFGVALESQRLFYRGKQMENGHTLFDYSVGLNDIVQLLVRQIPDSVPTKDKECGISDADSGCGSGQGESDKNSSCGEGATDVDGQPAGINSENVGPSLYKKNDLVDARDLNMGAWFEAQIVSVSKRVNPDGMSAEILDTSAASDDIIYHVKYEDYPENGVVQLTYKDVRLRARTTLPWHDLKVGQVVMVNYNPDEPKERGYWYDAEILRKRETRTIKEIYVKVLLGDAGDSLNDCRIRFVDEIYKIEEPGSAYITTESPQKRQNGPECKHCKDNPKRACRMCACYVCGGKQDPEKQLLCDECDMAFHIYCLKPPLSAIPQDEDWYCPDCRNDASEVVLAGEKLKESKKKAKMASASSSSQRDWGKGMACVGRSRECTIVPSNHYGPIPGVPVGTLWKFRVQVSESGVHRPHVAGIHGRSNDGSYSLVLAGGYEDDVDNGSEFTYTGSGGRDLSGNKRTAEQSCDQKLTNMNRALALNCSAPINDKEGAVAKDWRAGKPVRVVRNTKGKKHSKYAPEDGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDEEPAPWSKEGKERIKKLGLVMQYPDGYLESLASKEREKENKTEDELSESPSKGKRKRNSGSGLSDAKSTPKKTKVESYKLSLDQKTLIKQDDLNAKLWREVMSFLKEGPKFLSKVEETFLCICCQEVVYEPITTECHHNICKGCLDRSFKALVHNCPACRHDLGKNYSLNVNKPLQAILSQLFPGYERGR

Target ORF information:

RefSeq Version	XM_012963705.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963705.3

ORF Insert Sequence:

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC TGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uhrf1 ( XM_002940137.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940183.2 Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48333
Clone ID Related Accession (Same CDS sequence)	XM_012963705.3 , XM_002940137.5
Accession Version	XM_002940137.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UHRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940137.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC TGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940183.2
CDS	207..2531
Translation	MWIQVRTMDGRDTRRIDSLSKLTKVEDLRARIQQIFGVALESQRLFYRGKQMENGHTLFDYSVGLNDIVQLLVRQIPDSVPTKDKECGISDADSGCGSGQGESDKNSSCGEGATDVDGQPAGINSENVGPSLYKKNDLVDARDLNMGAWFEAQIVSVSKRVNPDGMSAEILDTSAASDDIIYHVKYEDYPENGVVQLTYKDVRLRARTTLPWHDLKVGQVVMVNYNPDEPKERGYWYDAEILRKRETRTIKEIYVKVLLGDAGDSLNDCRIRFVDEIYKIEEPGSAYITTESPQKRQNGPECKHCKDNPKRACRMCACYVCGGKQDPEKQLLCDECDMAFHIYCLKPPLSAIPQDEDWYCPDCRNDASEVVLAGEKLKESKKKAKMASASSSSQRDWGKGMACVGRSRECTIVPSNHYGPIPGVPVGTLWKFRVQVSESGVHRPHVAGIHGRSNDGSYSLVLAGGYEDDVDNGSEFTYTGSGGRDLSGNKRTAEQSCDQKLTNMNRALALNCSAPINDKEGAVAKDWRAGKPVRVVRNTKGKKHSKYAPEDGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDEEPAPWSKEGKERIKKLGLVMQYPDGYLESLASKEREKENKTEDELSESPSKGKRKRNSGSGLSDAKSTPKKTKVESYKLSLDQKTLIKQDDLNAKLWREVMSFLKEGPKFLSKVEETFLCICCQEVVYEPITTECHHNICKGCLDRSFKALVHNCPACRHDLGKNYSLNVNKPLQAILSQLFPGYERGR

Target ORF information:

RefSeq Version	XM_002940137.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin-like with PHD and ring finger domains 1 (uhrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940137.5

ORF Insert Sequence:

ATGTGGATTC AGGTGCGTAC AATGGATGGC CGAGACACTC GTCGTATTGA TTCTTTGTCA 
AAATTAACTA AAGTGGAAGA TCTAAGGGCG AGGATTCAGC AAATATTTGG GGTGGCATTG 
GAGAGTCAGC GTCTCTTCTA CAGAGGAAAG CAGATGGAAA ATGGACACAC ACTATTTGAT 
TACAGCGTTG GTTTGAATGA TATTGTTCAG CTGTTAGTAC GTCAGATTCC TGATTCTGTT 
CCCACAAAGG ATAAAGAGTG TGGGATTTCT GATGCGGATT CTGGTTGTGG TTCTGGACAG 
GGTGAATCTG ATAAGAATTC CAGTTGTGGT GAGGGTGCGA CGGATGTAGA TGGACAGCCT 
GCTGGCATAA ATAGTGAAAA TGTAGGACCT AGCCTGTATA AGAAAAATGA CTTAGTGGAT 
GCTCGAGATC TTAACATGGG TGCTTGGTTT GAGGCTCAAA TTGTAAGTGT CTCAAAGAGA 
GTGAATCCTG ATGGGATGTC AGCTGAAATC TTGGACACAT CTGCAGCTTC TGATGACATA 
ATCTATCATG TGAAATATGA GGATTATCCT GAAAATGGAG TTGTGCAGTT AACTTATAAG 
GATGTGCGGT TGCGAGCACG CACCACACTA CCATGGCATG ATCTTAAAGT TGGTCAGGTG 
GTCATGGTAA ATTATAATCC AGATGAGCCG AAAGAGAGAG GATACTGGTA TGATGCAGAA 
ATACTGCGGA AACGTGAGAC CAGAACAATT AAAGAAATTT ATGTCAAGGT GTTACTGGGG 
GATGCTGGTG ATTCCCTTAA TGACTGTAGA ATACGGTTTG TTGATGAAAT TTACAAAATA 
GAAGAGCCGG GATCTGCATA TATTACCACA GAGAGTCCTC AAAAACGTCA AAATGGTCCA 
GAGTGTAAAC ACTGCAAGGA CAACCCAAAG AGAGCCTGCC GGATGTGTGC ATGTTATGTT 
TGTGGAGGCA AACAGGACCC TGAAAAACAA CTACTTTGTG ATGAGTGTGA CATGGCATTC 
CATATTTACT GCCTTAAACC CCCATTGTCT GCCATACCAC AGGATGAAGA CTGGTACTGT 
CCAGACTGCA GAAATGATGC AAGCGAAGTG GTTCTAGCTG GTGAGAAGTT GAAAGAAAGC 
AAAAAGAAAG CCAAAATGGC ATCTGCTAGC TCCTCATCGC AGAGAGATTG GGGCAAGGGA 
ATGGCATGCG TAGGACGCTC CCGAGAATGC ACAATTGTTC CATCTAATCA TTATGGTCCA 
ATCCCTGGAG TACCTGTTGG AACACTATGG AAATTTAGAG TTCAAGTGAG TGAATCTGGA 
GTACACAGGC CACACGTGGC TGGTATACAT GGAAGAAGCA ATGATGGCTC ATATTCGTTG 
GTGCTGGCTG GAGGCTATGA AGATGATGTG GATAATGGAA GTGAGTTTAC TTATACTGGC 
AGTGGTGGTC GGGACCTTTC TGGAAACAAG CGAACAGCGG AACAGTCTTG TGACCAGAAA 
CTCACAAACA TGAACAGGGC TCTTGCTCTA AACTGCAGTG CACCTATAAA TGATAAAGAG 
GGAGCTGTTG CTAAGGATTG GCGAGCAGGC AAACCAGTTC GTGTAGTGCG TAATACAAAG 
GGTAAAAAAC ACAGTAAATA TGCTCCAGAG GATGGTAATC GCTATGATGG CATCTATAAG 
GTAGTAAAAT ACTGGCCAGA AAAAGGTAAA TCTGGGTTTT TGGTATGGCG ATACCTCCTA 
AGGAGAGATG ATGAAGAACC TGCACCCTGG TCAAAGGAAG GCAAGGAAAG GATTAAAAAA 
CTAGGTCTAG TCATGCAGTA TCCTGATGGT TACCTGGAAT CTCTTGCTAG CAAAGAGCGA 
GAAAAAGAAA ACAAGACAGA AGATGAGCTG AGCGAGTCAC CTAGCAAAGG GAAAAGGAAG 
CGAAACTCTG GTAGTGGACT GTCCGATGCC AAAAGCACTC CAAAGAAAAC AAAAGTGGAG 
TCCTACAAAC TAAGCCTGGA TCAGAAAACA CTTATAAAAC AAGATGACCT AAATGCAAAG 
TTATGGCGCG AAGTGATGTC ATTTCTGAAA GAAGGCCCTA AGTTTTTGAG CAAGGTGGAA 
GAAACATTCC TGTGCATCTG CTGTCAAGAA GTGGTATATG AACCTATTAC AACTGAATGC 
CATCATAATA TCTGCAAGGG GTGTCTTGAC CGTTCTTTCA AAGCATTGGT CCATAACTGC 
CCTGCTTGCC GGCATGATCT GGGAAAAAAC TACTCGCTAA ACGTGAACAA ACCACTGCAA 
GCTATCCTCA GCCAGTTGTT TCCAGGCTAC GAGAGAGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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uhrf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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