vwde cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following vwde gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vwde cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa60323 XM_002939011.5
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa60324 XM_012965592.3
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa60325 XM_012965594.3
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa60326 XM_031903928.1
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa12410 XM_002939011.4
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa36289 XM_012965592.2
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa36290 XM_012965594.2
Latest version!
Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa60323
    Clone ID Related Accession (Same CDS sequence) XM_002939011.5
    Accession Version XM_002939011.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939011.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT
    GTATGCTCAT GTCGCGCAGG ATACACTGGA ACAACTTGTA ACCAGAGAGT ACAGGGGTTT
    CGTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939057.3
    CDS241..5649
    Translation

    Target ORF information:

    RefSeq Version XM_002939011.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939011.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT
    GTATGCTCAT GTCGCGCAGG ATACACTGGA ACAACTTGTA ACCAGAGAGT ACAGGGGTTT
    CGTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60324
    Clone ID Related Accession (Same CDS sequence) XM_012965592.3
    Accession Version XM_012965592.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821046.2
    CDS242..5560
    Translation

    Target ORF information:

    RefSeq Version XM_012965592.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965592.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60325
    Clone ID Related Accession (Same CDS sequence) XM_012965594.3
    Accession Version XM_012965594.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
    CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT
    AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC
    TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT
    GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA
    GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA
    TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG
    GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA
    GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA
    GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC
    CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA
    ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA
    GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA
    GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA
    TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT
    TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC
    TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT
    GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC
    AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT
    GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT
    GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC
    CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA
    TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT
    GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA
    TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA
    GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC
    GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG
    TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT
    CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA
    GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG
    CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA
    AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC
    ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT
    CCTGAAAAGA AAGCTGTTAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA
    TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA
    GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG
    AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA
    ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC
    GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA
    TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC
    AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG
    GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT
    CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC
    TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA
    TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT
    GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT
    GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT
    GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT
    TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA
    CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT
    GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC
    CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT
    CCAGAAGGTT CTGCCATCCT TTTTACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC
    CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT
    CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT
    GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA
    TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA
    TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT
    GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC
    AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA
    TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG
    ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC
    TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA
    ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA
    AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG
    ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC
    AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT
    CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA
    CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT
    GCGAAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC
    AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC
    AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC
    AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT
    AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA
    GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA
    AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT
    AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT
    GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC
    ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG
    AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC
    ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC
    ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG
    AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA
    CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC
    AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA
    GGATACACTG GAACAACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821048.2
    CDS90..5240
    Translation

    Target ORF information:

    RefSeq Version XM_012965594.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965594.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
    CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT
    AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC
    TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT
    GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA
    GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA
    TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG
    GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA
    GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA
    GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC
    CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA
    ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA
    GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA
    GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA
    TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT
    TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC
    TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT
    GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC
    AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT
    GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT
    GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC
    CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA
    TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT
    GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA
    TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA
    GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC
    GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG
    TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT
    CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA
    GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG
    CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA
    AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC
    ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT
    CCTGAAAAGA AAGCTGTTAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA
    TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA
    GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG
    AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA
    ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC
    GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA
    TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC
    AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG
    GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT
    CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC
    TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA
    TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT
    GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT
    GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT
    GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT
    TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA
    CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT
    GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC
    CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT
    CCAGAAGGTT CTGCCATCCT TTTTACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC
    CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT
    CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT
    GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA
    TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA
    TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT
    GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC
    AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA
    TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG
    ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC
    TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA
    ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA
    AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG
    ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC
    AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT
    CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA
    CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT
    GCGAAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC
    AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC
    AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC
    AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT
    AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA
    GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA
    AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT
    AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT
    GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC
    ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG
    AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC
    ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC
    ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG
    AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA
    CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC
    AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA
    GGATACACTG GAACAACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60326
    Clone ID Related Accession (Same CDS sequence) XM_031903928.1
    Accession Version XM_031903928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGCA ATTTGCCGGC ATCCATGTGG AAAGAATATG
    GTATGCGCTG CTCCAAATGT TTGCAGATGT AGAAATGGCT ACTCTGGATA CAACTGCCAG
    ACTGCTGTGT GTCGTCCTGA CTGTAAGAAT CGTGGGAAAT GTGTGAGACC CAATGTTTGT
    GTATGCGCCC CAGGATATGG TGGAGAAACA TGTGAAGAAG CCTACTGTGA CCAACCATGT
    GAAAATGGAG GGACCTGCCA GGCAAGAAAT GTCTGCACCT GCCCATTTGG TTATGTTGGA
    CCCAGATGTG AAACAATGGT CTGTAATCGG CACTGTGAAA ATGGTGGCGA GTGTGTCGCT
    CCAGATGTGT GCAAGTGCAA ACCTGGGTGG TACGGCCCAA CATGCAGCAC AGCCATGTGT
    CATCCAGTTT GTCTTAATGG CGGCACTTGC ATGAAATCCA ATATTTGTCT GTGTCCCAAA
    GGATTCTATG GTGCCCAGTG TCAGAATGCG GTCTGTAGCC CTCCTTGCAA AAATGGCGGC
    CAGTGCATGA GAAATAACAT TTGCACGTGC CCAGATGGAT ACACTGGAAT AAGATGCCAG
    AAAAGTGTCT GCAATCCAAT GTGCATGAAT GGAGGGAAAT GTGTGGGGCC CAATATTTGT
    TCTTGCCCAT CAGGATGGAA AGGGAAGCAA TGCAATACAC CTATATGCCT TGAAAAATGT
    AAGAATGGAG GTGAGTGTGT GGGACCCAAT ACCTGCCACT GTCCAGCAGA GTGGGAAGGA
    AGCCAGTGCC AGACTGCTGT CTGCAACTAC AAATGTTTAT ATGGAGGCCG CTGTGTACTG
    CCCAATGTAT GCTCATGTCG CGCAGGATAC ACTGGAACAA CTTGTAACCA GAGAGTACAG
    GGGTTTCGTG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759788.1
    CDS242..5116
    Translation

    Target ORF information:

    RefSeq Version XM_031903928.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGCA ATTTGCCGGC ATCCATGTGG AAAGAATATG
    GTATGCGCTG CTCCAAATGT TTGCAGATGT AGAAATGGCT ACTCTGGATA CAACTGCCAG
    ACTGCTGTGT GTCGTCCTGA CTGTAAGAAT CGTGGGAAAT GTGTGAGACC CAATGTTTGT
    GTATGCGCCC CAGGATATGG TGGAGAAACA TGTGAAGAAG CCTACTGTGA CCAACCATGT
    GAAAATGGAG GGACCTGCCA GGCAAGAAAT GTCTGCACCT GCCCATTTGG TTATGTTGGA
    CCCAGATGTG AAACAATGGT CTGTAATCGG CACTGTGAAA ATGGTGGCGA GTGTGTCGCT
    CCAGATGTGT GCAAGTGCAA ACCTGGGTGG TACGGCCCAA CATGCAGCAC AGCCATGTGT
    CATCCAGTTT GTCTTAATGG CGGCACTTGC ATGAAATCCA ATATTTGTCT GTGTCCCAAA
    GGATTCTATG GTGCCCAGTG TCAGAATGCG GTCTGTAGCC CTCCTTGCAA AAATGGCGGC
    CAGTGCATGA GAAATAACAT TTGCACGTGC CCAGATGGAT ACACTGGAAT AAGATGCCAG
    AAAAGTGTCT GCAATCCAAT GTGCATGAAT GGAGGGAAAT GTGTGGGGCC CAATATTTGT
    TCTTGCCCAT CAGGATGGAA AGGGAAGCAA TGCAATACAC CTATATGCCT TGAAAAATGT
    AAGAATGGAG GTGAGTGTGT GGGACCCAAT ACCTGCCACT GTCCAGCAGA GTGGGAAGGA
    AGCCAGTGCC AGACTGCTGT CTGCAACTAC AAATGTTTAT ATGGAGGCCG CTGTGTACTG
    CCCAATGTAT GCTCATGTCG CGCAGGATAC ACTGGAACAA CTTGTAACCA GAGAGTACAG
    GGGTTTCGTG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12410
    Clone ID Related Accession (Same CDS sequence) XM_002939011.4
    Accession Version XM_002939011.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939011.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT
    GTATGCTCAT GTCGCGCAGG ATACACTGGA ACGACTTGTA ACCAGAGAGT ACAGGGGTTT
    CGTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939057.2
    CDS217..5625
    Translation

    Target ORF information:

    RefSeq Version XM_002939011.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939011.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT
    GTATGCTCAT GTCGCGCAGG ATACACTGGA ACGACTTGTA ACCAGAGAGT ACAGGGGTTT
    CGTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36289
    Clone ID Related Accession (Same CDS sequence) XM_012965592.2
    Accession Version XM_012965592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821046.1
    CDS217..5535
    Translation

    Target ORF information:

    RefSeq Version XM_012965592.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
    ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC
    CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT
    GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG
    CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA
    CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT
    TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG
    AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC
    TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC
    AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA
    GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC
    ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC
    AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA
    ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA
    CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC
    CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA
    GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA
    TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT
    CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT
    TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT
    CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA
    GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT
    GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT
    CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG
    TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC
    GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT
    AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA
    TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC
    TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT
    TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC
    AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA
    TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT
    AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA
    TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA
    ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA
    GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA
    ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT
    CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT
    TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT
    AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG
    CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC
    AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT
    CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG
    GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA
    TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT
    GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC
    TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT
    GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA
    CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA
    GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT
    GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG
    CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT
    GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA
    ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC
    TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT
    CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT
    CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT
    GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG
    CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG
    GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT
    CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC
    CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA
    GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT
    TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA
    TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG
    ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT
    GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT
    TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA
    ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG
    CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT
    GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG
    ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT
    GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG
    GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT
    ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC
    GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC
    GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT
    GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC
    GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT
    GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA
    TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT
    GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA
    GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC
    TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC
    ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT
    GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC
    CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT
    GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG
    TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36290
    Clone ID Related Accession (Same CDS sequence) XM_012965594.2
    Accession Version XM_012965594.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
    CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT
    AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC
    TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT
    GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA
    GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA
    TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG
    GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA
    GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA
    GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC
    CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA
    ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA
    GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA
    GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA
    TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT
    TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC
    TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT
    GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC
    AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT
    GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT
    GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC
    CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA
    TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT
    GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA
    TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA
    GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC
    GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG
    TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT
    CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA
    GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG
    CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA
    AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC
    ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT
    CCTGAAAAGA AAGCTGATAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA
    TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA
    GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG
    AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA
    ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC
    GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA
    TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC
    AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG
    GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT
    CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC
    TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA
    TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT
    GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT
    GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT
    GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT
    TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA
    CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT
    GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC
    CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT
    CCAGAAGGTT CTGCCATCCT TTTCACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC
    CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT
    CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT
    GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA
    TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA
    TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT
    GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC
    AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA
    TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG
    ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC
    TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA
    ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA
    AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG
    ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC
    AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT
    CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA
    CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT
    GTGGAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC
    AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC
    AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC
    AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT
    AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA
    GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA
    AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT
    AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT
    GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC
    ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG
    AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC
    ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC
    ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG
    AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA
    CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC
    AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA
    GGATACACTG GAACGACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821048.1
    CDS90..5240
    Translation

    Target ORF information:

    RefSeq Version XM_012965594.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965594.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
    CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT
    AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC
    TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT
    GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA
    GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA
    TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG
    GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA
    GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA
    GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC
    CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA
    ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA
    GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA
    GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA
    TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT
    TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC
    TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT
    GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC
    AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT
    GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT
    GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC
    CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA
    TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT
    GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA
    TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA
    GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC
    GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG
    TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT
    CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA
    GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG
    CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA
    AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC
    ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT
    CCTGAAAAGA AAGCTGATAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA
    TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA
    GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG
    AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA
    ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC
    GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA
    TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC
    AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG
    GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT
    CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC
    TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA
    TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT
    GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT
    GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT
    GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT
    TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA
    CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT
    GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC
    CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT
    CCAGAAGGTT CTGCCATCCT TTTCACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC
    CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT
    CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT
    GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA
    TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA
    TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT
    GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC
    AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA
    TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG
    ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC
    TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA
    ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA
    AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG
    ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC
    AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT
    CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA
    CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT
    GTGGAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC
    AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC
    AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC
    AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT
    AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA
    GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA
    AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT
    AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT
    GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC
    ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG
    AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC
    ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC
    ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG
    AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA
    CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC
    AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA
    GGATACACTG GAACGACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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