LOC100273009 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100273009
Entrez Gene ID	100273009
Full Name	Ascorbate-specific transmembrane electron transporter 1
Synonyms	GRMZM2G060357,Zmb561
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001147459.1	NP_001140931.1	ascorbate-specific transmembrane electron transporter 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of electron acceptance from ascorbate by the specific N-carbethoxylations of maize cytochrome b561: a common mechanism for the transmembrane electron transfer in cytochrome b561 protein family.
Nakanishi N, Rahman MM, Sakamoto Y, Miura M, Takeuchi F, Park SY, Tsubaki M
Journal of biochemistry146(6)857-66(2009 Dec)

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

Importance of the conserved lysine 83 residue of Zea mays cytochrome b(561) for ascorbate-specific transmembrane electron transfer as revealed by site-directed mutagenesis studies.
Nakanishi N, Rahman MM, Sakamoto Y, Takigami T, Kobayashi K, Hori H, Hase T, Park SY, Tsubaki M
Biochemistry48(44)10665-78(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100273009 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100273009 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa01436	NM_001147459.1 Latest version!	Zea mays uncharacterized LOC100273009 (LOC100273009), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100273009 ( NM_001147459.1 ) cDNA ORF clone, Zea mays -> NP_001140931.1 Zea mays uncharacterized LOC100273009 (LOC100273009), mRNA.

Price & Availability↑

CloneID	OZa01436
Clone ID Related Accession (Same CDS sequence)	NM_001147459.1
Accession Version	NM_001147459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Zea mays
Product	ascorbate-specific transmembrane electron transporter 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT039983.1. ##Evidence-Data-START## Transcript exon combination :: BT039983.1, SRR3147046.145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN01096209 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTTG GCCTTGGCGT GCGGGCGGCG CCCTTCACGT ACGCGGCGCA CGCACTGGCC 
GTCGCGGCGG CGGCCATGGT TCTCGTCTGG TCCATCCAGT TCCGCGGTGG GCTCGCCATC 
GAGTCCACCA ACAAGAACCT CATCTTCAAC GTTCACCCTG TTCTTATGCT TATCGGTTAT 
GTTATTATCG GCGGTGAAGC TATAATGGTG TACAGGGTAC TTCCAACATC GAATCATGAC 
ACAACTAAGC TGATCCATCT AATTCTCCAC GGGATTGCAC TCGTCCTTGG TGCGGTTGGA 
ATATACTTTG CTTTCAAAAA TCACAATGAG AGTGGGATTG CCAATCTTTA CAGCCTGCAC 
TCTTGGATTG GGATAGGAAC AATTACTTTG TATGGTATTC AGTGGATAAT TGGATTCGTC 
ACGTTCTTCT TCCCTGGTGC TGCGCCAAAC GTGAAGAAGG GTGTTCTTCC TTGGCACGTA 
CTCTTTGGGC TCTTTGTCTA CATCTTAGCA CTGGCTAATG CAGAACTCGG CTTTCTTGAG 
AAGCTCACTT TCCTCGAGAG CTCAGGTCTC GACAAATATG GCACAGAGGC ATTCTTAGTG 
AACTTCACAG CATTGGTTGT CGTGTTATTT GGTGCCTCTG TTGTGGTGGC TGCTATAGCT 
CCAGTTCGCC TTGAAGAACC ACAGGGTTAT GATCCAATTC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001140931.1
CDS	111..821
Translation	MGLGLGVRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAPVRLEEPQGYDPIPEN

Target ORF information:

RefSeq Version	NM_001147459.1
Organism	Zea mays
Definition	Zea mays uncharacterized LOC100273009 (LOC100273009), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001147459.1

ORF Insert Sequence:

ATGGGGCTTG GCCTTGGCGT GCGGGCGGCG CCCTTCACGT ACGCGGCGCA CGCACTGGCC 
GTCGCGGCGG CGGCCATGGT TCTCGTCTGG TCCATCCAGT TCCGCGGTGG GCTCGCCATC 
GAGTCCACCA ACAAGAACCT CATCTTCAAC GTTCACCCTG TTCTTATGCT TATCGGTTAT 
GTTATTATCG GCGGTGAAGC TATAATGGTG TACAGGGTAC TTCCAACATC GAATCATGAC 
ACAACTAAGC TGATCCATCT AATTCTCCAC GGGATTGCAC TCGTCCTTGG TGCGGTTGGA 
ATATACTTTG CTTTCAAAAA TCACAATGAG AGTGGGATTG CCAATCTTTA CAGCCTGCAC 
TCTTGGATTG GGATAGGAAC AATTACTTTG TATGGTATTC AGTGGATAAT TGGATTCGTC 
ACGTTCTTCT TCCCTGGTGC TGCGCCAAAC GTGAAGAAGG GTGTTCTTCC TTGGCACGTA 
CTCTTTGGGC TCTTTGTCTA CATCTTAGCA CTGGCTAATG CAGAACTCGG CTTTCTTGAG 
AAGCTCACTT TCCTCGAGAG CTCAGGTCTC GACAAATATG GCACAGAGGC ATTCTTAGTG 
AACTTCACAG CATTGGTTGT CGTGTTATTT GGTGCCTCTG TTGTGGTGGC TGCTATAGCT 
CCAGTTCGCC TTGAAGAACC ACAGGGTTAT GATCCAATTC CAGAAAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of electron acceptance from ascorbate by the specific N-carbethoxylations of maize cytochrome b561: a common mechanism for the transmembrane electron transfer in cytochrome b561 protein family.
Journal of biochemistry146(6)857-66(2009 Dec)
Nakanishi N,Rahman MM,Sakamoto Y,Miura M,Takeuchi F,Park SY,Tsubaki M

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

Importance of the conserved lysine 83 residue of Zea mays cytochrome b(561) for ascorbate-specific transmembrane electron transfer as revealed by site-directed mutagenesis studies.
Biochemistry48(44)10665-78(2009 Nov)
Nakanishi N,Rahman MM,Sakamoto Y,Takigami T,Kobayashi K,Hori H,Hase T,Park SY,Tsubaki M

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LOC100273009 cDNA ORF clone, Zea mays

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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