Gene Symbol | LOC541812 |
Entrez Gene ID | 541812 |
Full Name | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic |
Synonyms | GRMZM2G132021 |
Gene Type | protein-coding |
Organism | Zea mays |
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ORF » Species Summary » Zea mays » LOC541812 cDNA ORF clone
mRNA | Protein | Name |
---|---|---|
XM_020542317.1 | XP_020397906.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X1 |
XM_020542318.1 | XP_020397907.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X2 |
XM_020542318.2 | XP_020397907.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X2 |
XM_020542320.1 | XP_020397909.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X4 |
XM_020542319.2 | XP_020397908.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X3 |
XM_020542320.2 | XP_020397909.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X4 |
XM_020542319.1 | XP_020397908.1 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X3 |
NM_001111493.2 | NP_001104963.2 | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic precursor |
Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2.
Ostheimer GJ, Rojas M, Hadjivassiliou H, Barkan A
The Journal of biological chemistry281(8)4732-8(2006 Feb)
Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces.
Ostheimer GJ, Hadjivassiliou H, Hadjivasiliou H, Kloer DP, Barkan A, Matthews BW
Journal of molecular biology345(1)51-68(2005 Jan)
Group II intron splicing factors derived by diversification of an ancient RNA-binding domain.
Ostheimer GJ, Williams-Carrier R, Belcher S, Osborne E, Gierke J, Barkan A
The EMBO journal22(15)3919-29(2003 Aug)
Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts.
Jenkins BD, Barkan A
The EMBO journal20(4)872-9(2001 Feb)
Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants.
Vogel J, B?rner T, Hess WR
Nucleic acids research27(19)3866-74(1999 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
a 22-amino acid motif in the COOH-terminal region of CAF2 mediates formation of the CRS2-CAF2 group II intron splicing complex
Title: Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2.
The following LOC541812 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC541812 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OZa65016 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542317.1 , NM_001111493.2 | |
Accession Version | NM_001111493.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-05 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BT036053.1 and BT038431.1. On Sep 16, 2016 this sequence version replaced NM_001111493.1. ##Evidence-Data-START## Transcript exon combination :: BT038431.1, AF225708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001104963.2 |
CDS | 93..863 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65016 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542317.1 , NM_001111493.2 | |
Accession Version | XM_020542317.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-12-17 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397906.1 |
CDS | 102..872 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65017 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542318.1 , XM_020542318.2 | |
Accession Version | XM_020542318.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-03-19 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X2 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397907.1 |
CDS | 104..802 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65017 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542318.1 , XM_020542318.2 | |
Accession Version | XM_020542318.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-12-17 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020542318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397907.1 |
CDS | 103..801 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65018 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542319.2 , XM_020542319.1 | |
Accession Version | XM_020542319.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-12-17 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020542319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397908.1 |
CDS | 103..777 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65018 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542319.2 , XM_020542319.1 | |
Accession Version | XM_020542319.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-03-19 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X3 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397908.1 |
CDS | 104..778 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65019 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542320.1 , XM_020542320.2 | |
Accession Version | XM_020542320.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-03-19 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X4 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397909.1 |
CDS | 104..763 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OZa65019 | |
Clone ID Related Accession (Same CDS sequence) | XM_020542320.1 , XM_020542320.2 | |
Accession Version | XM_020542320.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-12-17 | |
Organism | Zea mays | |
Product | chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024466.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020542320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_020397909.1 |
CDS | 103..762 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCGTTGC TCGCCGCGGC CATTCCCAGC ACTTCCTCCC ACTTCTCCGC ACCCTTCCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2. |
Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces. |
Group II intron splicing factors derived by diversification of an ancient RNA-binding domain. |
Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts. |
Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants. |