LOC542754 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC542754
Entrez Gene ID	542754
Full Name	terpene synthase 4
Synonyms	GRMZM2G117319,TPS5,tps4-B73
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305938.2	NP_001292867.2	terpene synthase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Substrate geometry controls the cyclization cascade in multiproduct terpene synthases from Zea mays.
Vattekkatte A, Gatto N, K?llner TG, Degenhardt J, Gershenzon J, Boland W
Organic & biomolecular chemistry13(21)6021-30(2015 Jun)

Isotope sensitive branching and kinetic isotope effects to analyse multiproduct terpenoid synthases from Zea mays.
Gatto N, Vattekkatte A, K?llner T, Degenhardt J, Gershenzon J, Boland W
Chemical communications (Cambridge, England)51(18)3797-800(2015 Mar)

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

Two pockets in the active site of maize sesquiterpene synthase TPS4 carry out sequential parts of the reaction scheme resulting in multiple products.
K?llner TG, O'Maille PE, Gatto N, Boland W, Gershenzon J, Degenhardt J
Archives of biochemistry and biophysics448(1-2)83-92(2006 Apr)

The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes.
K?llner TG, Schnee C, Gershenzon J, Degenhardt J
The Plant cell16(5)1115-31(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Substrate geometry controls the cyclization cascade in multiproduct terpene synthases TPS4 and TPS5 from Zea mays.
Title: Substrate geometry controls the cyclization cascade in multiproduct terpene synthases from Zea mays.

The following LOC542754 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC542754 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa22685	NM_001305938.2 Latest version!	Zea mays terpene synthase 4 (LOC542754), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC542754 ( NM_001305938.2 ) cDNA ORF clone, Zea mays -> NP_001292867.2 Zea mays terpene synthase 4 (LOC542754), mRNA.

Price & Availability↑

CloneID	OZa22685
Clone ID Related Accession (Same CDS sequence)	NM_001305938.2
Accession Version	NM_001305938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-13
Organism	Zea mays
Product	terpene synthase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY518310.1. On or before Apr 27, 2015 this sequence version replaced NM_001112263.1, NM_001305938.1. ##Evidence-Data-START## Transcript exon combination :: AY518310.1, AY518312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780135 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCTC CTCCAGCACA TCGTTCCAGC AAGGCAGCTG ATGAGGAGCT GCCAAAGGCT 
TCTTCGACCT TTCACCCAAG TCTGTGGGGC AGTTTCTTCC TGACCTACCA GCCGCCAACT 
GCACCTCAGC GAGCAAACAT GAAAGAACGA GCTGAGGTTT TAAGAGAACG TGTTAGGAAG 
GTTTTGAAGG GCTCGACGAC TGATCAACTA CCAGAAACAG TGAATCTTAT ATTAACATTG 
CAAAGACTTG GACTGGGTTA CTACTACGAG AACGAGATAG ACAAGTTGCT GCACCAAATT 
TACAGCAACT CCGATTACAA TGTGAAAGAT CTTAACTTGG TTTCGCAGCG GTTCTACCTT 
CTTCGAAAGA ACGGCTACGA TGTGCCATCA GATGTATTTC TGAGCTTCAA AACAGAAGAA 
GGCGGCTTCG CCTGTGCTGC TGCTGACACG AGAAGCTTGT TAAGCTTATA TAACGCAGCA 
TATCTGAGGA AGCATGGAGA GGAGGTACTA GACGAAGCGA TTTCATCGAC TAGACTCCGC 
CTCCAAGATC TCCTTGGACG ACTTCTTCCA GAATCGCCAT TTGCAAAGGA GGTGTCTTCT 
TCCCTTCGTA CCCCTCTCTT CAGAAGGGTT GGGATACTAG AAGCGAGAAA CTACATACCC 
ATTTACGAAA CGGAGGCGAC GCGAAACGAA GCCGTATTGG AGCTTGCGAA ACTGAATTTT 
AACCTTCAGC AACTTGATTT CTGTGAAGAG CTAAAGCATT GCTCAGCGTG GTGGAACGAG 
ATGATAGCCA AATCAAAGCT GACCTTTGTT AGAGATAGAA TTGTAGAGGA GTATTTCTGG 
ATGAATGGAG CATGCTATGA TCCGCCGTAC TCCCTTTCCC GAATTATACT GACAAAGATC 
ACGGGACTTA TTACAATAAT CGATGATATG TTTGATACAC ATGGTACCAC TGAAGATTGC 
ATGAAATTCG CTGAAGCGTT TGGCAGATGG GACGAAAGTG CAATACATCT CCTTCCAGAG 
TACATGAAGG ATTTCTACAT ATTGATGTTG GAGACATTTC AGTCATTTGA GGATGCTTTA 
GGGCCAGAGA AGAGCTACCG TGTGCTTTAC CTAAAACAGG CGATGGAGCG TTTAGTTGAG 
CTATACTCCA AGGAAATAAA ATGGCGTGAT GATGATTATG TGCCGACAAT GAGTGAACAC 
CTCCAGGTTT CAGCAGAGAC CATCGCAACC ATTGCTCTAA CATGTTCTGC ATATGCTGGG 
ATGGGTGATA TGTCGATAAG AAAGGAGACC TTCGAGTGGG CTTTGAGCTT TCCTCAATTT 
ATAAGAACTT TTGGTTCATT TGTACGGCTC TCCAACGATG TCGTATCGAC CAAGCGTGAG 
CAAACAAAAG ACCATAGTCC CTCCACTGTC CATTGTTACA TGAAGGAGCA TGGAACGACC 
ATGGACGATG CATGTGAAAA GATAAAGGAG CTTATCGAAG ATTCATGGAA GGATATGCTA 
GAGCAATCCC TTGCGCTGAA AGGGCTCCCA AAGGTTGTGC CACAGCTAGT GTTTGACTTC 
TCAAGAACCA CGGATAATAT GTACAGGGAT CGTGACGCAT TGACCTCTTC GGAAGCACTC 
AAGGAAATGA TACAGCTACT GTTTGTGGAG CCAATACCCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292867.2
CDS	1..1665
Translation	MASPPAHRSSKAADEELPKASSTFHPSLWGSFFLTYQPPTAPQRANMKERAEVLRERVRKVLKGSTTDQLPETVNLILTLQRLGLGYYYENEIDKLLHQIYSNSDYNVKDLNLVSQRFYLLRKNGYDVPSDVFLSFKTEEGGFACAAADTRSLLSLYNAAYLRKHGEEVLDEAISSTRLRLQDLLGRLLPESPFAKEVSSSLRTPLFRRVGILEARNYIPIYETEATRNEAVLELAKLNFNLQQLDFCEELKHCSAWWNEMIAKSKLTFVRDRIVEEYFWMNGACYDPPYSLSRIILTKITGLITIIDDMFDTHGTTEDCMKFAEAFGRWDESAIHLLPEYMKDFYILMLETFQSFEDALGPEKSYRVLYLKQAMERLVELYSKEIKWRDDDYVPTMSEHLQVSAETIATIALTCSAYAGMGDMSIRKETFEWALSFPQFIRTFGSFVRLSNDVVSTKREQTKDHSPSTVHCYMKEHGTTMDDACEKIKELIEDSWKDMLEQSLALKGLPKVVPQLVFDFSRTTDNMYRDRDALTSSEALKEMIQLLFVEPIPE

Target ORF information:

RefSeq Version	NM_001305938.2
Organism	Zea mays
Definition	Zea mays terpene synthase 4 (LOC542754), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305938.2

ORF Insert Sequence:

ATGGCGTCTC CTCCAGCACA TCGTTCCAGC AAGGCAGCTG ATGAGGAGCT GCCAAAGGCT 
TCTTCGACCT TTCACCCAAG TCTGTGGGGC AGTTTCTTCC TGACCTACCA GCCGCCAACT 
GCACCTCAGC GAGCAAACAT GAAAGAACGA GCTGAGGTTT TAAGAGAACG TGTTAGGAAG 
GTTTTGAAGG GCTCGACGAC TGATCAACTA CCAGAAACAG TGAATCTTAT ATTAACATTG 
CAAAGACTTG GACTGGGTTA CTACTACGAG AACGAGATAG ACAAGTTGCT GCACCAAATT 
TACAGCAACT CCGATTACAA TGTGAAAGAT CTTAACTTGG TTTCGCAGCG GTTCTACCTT 
CTTCGAAAGA ACGGCTACGA TGTGCCATCA GATGTATTTC TGAGCTTCAA AACAGAAGAA 
GGCGGCTTCG CCTGTGCTGC TGCTGACACG AGAAGCTTGT TAAGCTTATA TAACGCAGCA 
TATCTGAGGA AGCATGGAGA GGAGGTACTA GACGAAGCGA TTTCATCGAC TAGACTCCGC 
CTCCAAGATC TCCTTGGACG ACTTCTTCCA GAATCGCCAT TTGCAAAGGA GGTGTCTTCT 
TCCCTTCGTA CCCCTCTCTT CAGAAGGGTT GGGATACTAG AAGCGAGAAA CTACATACCC 
ATTTACGAAA CGGAGGCGAC GCGAAACGAA GCCGTATTGG AGCTTGCGAA ACTGAATTTT 
AACCTTCAGC AACTTGATTT CTGTGAAGAG CTAAAGCATT GCTCAGCGTG GTGGAACGAG 
ATGATAGCCA AATCAAAGCT GACCTTTGTT AGAGATAGAA TTGTAGAGGA GTATTTCTGG 
ATGAATGGAG CATGCTATGA TCCGCCGTAC TCCCTTTCCC GAATTATACT GACAAAGATC 
ACGGGACTTA TTACAATAAT CGATGATATG TTTGATACAC ATGGTACCAC TGAAGATTGC 
ATGAAATTCG CTGAAGCGTT TGGCAGATGG GACGAAAGTG CAATACATCT CCTTCCAGAG 
TACATGAAGG ATTTCTACAT ATTGATGTTG GAGACATTTC AGTCATTTGA GGATGCTTTA 
GGGCCAGAGA AGAGCTACCG TGTGCTTTAC CTAAAACAGG CGATGGAGCG TTTAGTTGAG 
CTATACTCCA AGGAAATAAA ATGGCGTGAT GATGATTATG TGCCGACAAT GAGTGAACAC 
CTCCAGGTTT CAGCAGAGAC CATCGCAACC ATTGCTCTAA CATGTTCTGC ATATGCTGGG 
ATGGGTGATA TGTCGATAAG AAAGGAGACC TTCGAGTGGG CTTTGAGCTT TCCTCAATTT 
ATAAGAACTT TTGGTTCATT TGTACGGCTC TCCAACGATG TCGTATCGAC CAAGCGTGAG 
CAAACAAAAG ACCATAGTCC CTCCACTGTC CATTGTTACA TGAAGGAGCA TGGAACGACC 
ATGGACGATG CATGTGAAAA GATAAAGGAG CTTATCGAAG ATTCATGGAA GGATATGCTA 
GAGCAATCCC TTGCGCTGAA AGGGCTCCCA AAGGTTGTGC CACAGCTAGT GTTTGACTTC 
TCAAGAACCA CGGATAATAT GTACAGGGAT CGTGACGCAT TGACCTCTTC GGAAGCACTC 
AAGGAAATGA TACAGCTACT GTTTGTGGAG CCAATACCCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substrate geometry controls the cyclization cascade in multiproduct terpene synthases from Zea mays.
Organic & biomolecular chemistry13(21)6021-30(2015 Jun)
Vattekkatte A,Gatto N,K?llner TG,Degenhardt J,Gershenzon J,Boland W

Isotope sensitive branching and kinetic isotope effects to analyse multiproduct terpenoid synthases from Zea mays.
Chemical communications (Cambridge, England)51(18)3797-800(2015 Mar)
Gatto N,Vattekkatte A,K?llner T,Degenhardt J,Gershenzon J,Boland W

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

Two pockets in the active site of maize sesquiterpene synthase TPS4 carry out sequential parts of the reaction scheme resulting in multiple products.
Archives of biochemistry and biophysics448(1-2)83-92(2006 Apr)
K?llner TG,O'Maille PE,Gatto N,Boland W,Gershenzon J,Degenhardt J

The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes.
The Plant cell16(5)1115-31(2004 May)
K?llner TG,Schnee C,Gershenzon J,Degenhardt J

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LOC542754 cDNA ORF clone, Zea mays

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