Genomic DNA Sequencing
CRISPR gene edited mammalian cell lines play an important role for a variety of applications, from drug discovery, establishment of primary cell lines, to epigenetic studies. While CRISPR is considered to be one of the most efficient and accurate editing methods, off-targeting can still be an issue, and therefore verification of gene knock-in and knock-out is a critical component of CRISPR quality control. To make this process even easier, GenScript offers a genomic DNA sequencing service to ensure CRISPR specificity in modified single cell clones or pools.
Advantages of genomic DNA sequencing:
- Less expensive: while Next Generation Sequencing (NGS) may be the most comprehensive, it is expensive and unnecessary for verifying single cell clones or pools
- Less labor intensive: PCR and sanger sequencing require expertise and very labor intensive
- Shorter turnaround time compared to NGS
Service details
| Service | Deliverables | Technique | Delivery | Turnaround time | Price* |
|---|---|---|---|---|---|
| Single clone Genomic DNA Sequencing |
AB1 files Alignment files |
PCR Sequencing | 1-2 effective sequencing results | 2 weeks | $80 |
| Pooled clone genomic DNA sequencing | AB1 files Alignment files Mutation statistic report |
PCR, TA Cloning, plasmid prep and sequencing | ≥20 effective sequencing results | 3 weeks | $500 |
*Pricing per target, up to 800bp
How to order
- - Choose the desired service:
- - Single clone sequencing: for clones with a single mutation
- - Pooled clone sequencing: for mixtures of clones with multiple mutations
- - Email us with the genomic DNA and reference sequences or accession #
Have questions? GenScript's Ph.D.-level service representatives are available 24 hours a day, Monday through Friday, to assist you.