At GenScript, our GenTitan™ Oligo Pool service offers researchers a comprehensive solution for customized oligonucleotide pools. Using our proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology, we achieve unmatched precision via microelectrode control, ensuring even distribution and minimal batch differences. Harnessing the industry's largest capacity, with over 8 million oligos on a single chip, our technology empowers breakthroughs in gene editing, protein/antibody engineering, DNA data storage, and more.
Consistency
Complete chamber reaction for excellent uniformity
Precision
Precise control of current and voltage at each electrode
Capacity
Up to 8 Million Oligos on a single chip
Discover how our Next-Generation CMOS Technology is pushing the boundaries of DNA Synthesis.
Miniature Semiconductor Chip (MSC) enables large-scale de novo synthesis of DNA using proprietary electrochemistry technologies and delivers precision on a microelectrode scale.
ACCURATE SPATIAL CONTROL
Semiconductor based ensures spatial control over the whole process
ENCLOSED ENVIRONMENT
Less interference of H2O, O2, O3 and static electricity
CONSISTENCY & UNIFORMITY
Full chamber reaction with standard wafer and chip production process
We Offer Customized Pricing to Match your Needs (Starting from 1K pool size). Get an Estimated Price with Our Calculator Today!
Oligo Length (10-170nt)
Pool Size (0K - 8M)
Genome Editing
CRISPR gRNA Library
Oligo pool gRNA libraries, utilizing CRISPR gene editing, enable high-throughput screening for cancer therapy, diagnostics, and microorganism engineering. They facilitate gene knock-out and activation, probing tumorigenesis, biomarkers, and metabolic pathways. This transformative tool leverages CRISPR's precision for versatile biomedical and biotech applications.
shRNA Library
A pooled lentiviral screen can be performed to identify genes regulating cellular responses and signaling pathways, or to uncover novel functions. Libraries can comprise 50 to thousands of constructs. GenScript offers lentivirus-based RNAi libraries through our siRNA Design Center and chip-based synthesis platform, supporting research hypothesis verification.
NGS Target Enrichment
Next-generation sequencing (NGS) target enrichment utilizing oligo pools has emerged as a transformative technique in genomics research. By custom designing pools of oligonucleotide probes complementary to specific genomic regions of interest, researchers can efficiently capture and amplify relevant DNA fragments for sequencing. This approach not only streamlines the sequencing process but also significantly reduces the cost and data burden.
Protein & Antibody Engineering
Mutant libraries are powerful, high-throughput tools for optimizing protein structure and function. However, traditional methods for library construction, including error-prone PCR or degenerate methods, suffer from limited control over codon usage and poor variant representation. Powered by our semiconductor-based oligo synthesis technology, our Precision Mutant Library service offers precise control over each synthesized variant. The result is a more diverse and fully covered mutant library with unbiased distribution.
Library services we offer:
Gene Fragments
Gene Fragments offer a cost-effective, swift, and effective avenue for constructing the requisite genes in your research endeavors. These double-stranded DNA fragments of up to 1,800 base pairs in length, fulfilling the demands of easy gene construction or modification suitable for a variety of applications, including antibody development, CRISPR-mediated genome editing, and NGS controls. The GenTitan™ Gene Fragments Service leverages our exclusive complementary metal oxide semiconductor (CMOS) integrated circuits (IC), ensuring unmatched precision, accelerated delivery schedules, and exceptional cost-effectiveness.
DNA Data Storage
DNA data storage involves encoding digital information as DNA sequences, utilizing the four DNA bases (A, T, C, and G) to represent binary data (0s and 1s). These sequences can be later synthesized into DNA strands for storage. Oligo pools are used to generate these sequences efficiently and in parallel, allowing large amounts of data to be encoded using unique combinations of oligos.
GenTitan benefits:
PCR Enrichment and Amplification Guidelines for GenTitan™ Oligo Pools
Free Download
Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools
Free Download
Double stranded DNA capture probes in NGS targeted sequencing
Free Download
Assembly of precise mutant libraries using arrayed-synthesized oligos
Free Download
Cost-effective library construction and efficient high-throughput screening
Free DownloadGenTitan™ oligo pools are collections of oligonucleotides synthesized using GenScript’s proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology. GenTitan™ oligo pools are designed for PCR (Polymerase Chain Reaction) amplification, which requires that the oligos be carefully designed with specific sequences at their ends to ensure effective primer annealing and successful amplification.
GenScript guarantees that when an oligo pool is appropriately designed and used as a template for PCR with suitable primers, it will produce a PCR product of the correct size. However, it's important to note that while general PCR guidelines are provided, modifications to the PCR protocols might be necessary to accommodate specific variables, such as the choice of primers and the presence of certain sequence motifs. As every pool is uniquely made according to the customer’s design, GenScript cannot commit to specific characteristics that could be influenced by the sequence design or downstream workflow.
| GenTitan™Oligo Pools | |
|---|---|
| Chip Size | 8 Million Oligos on 1 Chip |
| Oligo Length | Up to 170nt |
| Yield | Up to 1 fmol for each oligo |
| Minimum Order | Pricing option starting from 1K pools |
| Delivery Time | 1-2 weeks |
| Quality Control |
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*PCR amplification of entire pool, cloning, and sequencing for sequence coverage and accuracy at an additional fee*
New – Synthesis verification of full oligo length using spike-in control oligos
In order to verify full-length synthesis of oligos, we now offer an additional QC option for FREE, PCR amplification of control oligos synthesized on the same chip . Sequences of 50 optimized control oligos will be synthesized with each pool and PCR amplification of the control oligos will be performed to confirm oligo synthesis. Customer sequences will be screened to ensure QC PCR primers will only capture control oligos.
Synthesis Verification of Full Length Oligos in a Pool
