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Glutamine Synthetase Gene Expression Technology

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The Glutamine Synthetase gene expression is widely used for recombinant antibody and protein production in mammalian cell culture. Glutamine Synthetase gene expression relies on glutamine synthetase enzyme which synthesizes glutamine from glutamate and ammonium ions. Since many mammalian cells cannot make endogenous glutamine, transfection with a vector encoding Glutamine Synthetase enzyme ensures cell survival in culture. And for those cells that make endogenous glutamine, using the Glutamine Synthetase enzyme inhibitor, methionine sulfoximine (MSX), enables suppression of the endogenous glutamine while allowing use of the Glutamine Synthetase vector for recombinant expression. To learn more about the Glutamine Synthetase technology principle, click here.

GenScript's Glutamine Synthetase technology uses a proprietary vector with a strong promoter to drive recombinant gene expression and a relatively weaker promoter that drives the Glutamine Synthetase gene expression. The reliability of the Glutamine Synthetase gene expression will allow the rapid generation of high-producing cell lines. To see Glutamine Synthetase technology case studies, click here. For GenScript Glutamine Synthetase gene expression details, see below.

Key Features and Advantages of GenScript Glutamine Synthetase Technology

GenScript Glutamine Synthetase Technology Workflow

GenScript Glutamine Synthetase Technology Package Details

Step	Description	Timeline (weeks)	Deliverables	Pricing
Molecular Biology	Codon optimization Gene synthesis Cloning Transfection-grade DNA prep	2-4	Target gene(s) in pUC57 and vector map Expression report Top 3 stable clones Comprehensive report	REQUEST NOW
Stable cell pool screening	Transfection Stable cell pool screening Stable cell pool detection Selection of top 3 cell pools	6-8
Sub-cloning & limiting dilution	Screening 100-300 single cell clones Single cell clone detection	4
Batch culture or Fed-batch culture	Select top 10-30 clones based on expression profile, for batch culture or fed-batch culture	3-4
Cell line stability evaluation	Select top 5-10 clones based on expression profile, for stability test, 10 generations Mycoplasma testing using NEST-PCR	2
Research Cell Bank Preparation	Preparation of frozen cells from top 3 clones for delivery (20 vials/clone)	2
Scale up protein or rAb production	Scale up and purification of rAb or protein, as per customer request	Variable, based on request

For details on GenScript's overall stable cell line service portfolio, click here.

Not what you are looking for? See our related service pages below.

• Prefer to check on your protein expression level first? Check out our PROTential™ protein expression evaluation & optimization service. PROTential™ helps to check on the target protein expression in CHO or HEK293 cells, starting at just $500.
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• Need high quality recombinant antibody up to 500mg at ≥95% purity and with low endotoxin levels? Visit our MamPower™ guaranteed recombinant antibody production page.
• Need more than 500mg of recombinant antibody? Visit our large scale recombinant antibody production page.
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• For information about our protein services, please visit our recombinant protein services main page or contact us using the information listed below.
• Unsure which rAb service would work best for you? Use this guide to quickly determine which services are the best match for your application.

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