Assay Cell Line Development: Gene Over-Expression and Functional Assays
Recombinant stable cell lines are one of the widely used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest (GOI) is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the GOI together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell line offers reproducible results for assay studies.
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After selection, cells that have the expression cassette incorporated into the genome will be enriched, resulting in stable pool cells. By clonal selection from the stable pool cells, single clone cells are generated. Both stable pools and single clones are frequently used in research.
Recombinant stable cell line development at GenScript includes over-expression, inducible expressionI. In addition, we developed lentivirus based CRISPR/Cas (Lenti-CRISPR/Cas9) technologies and platforms, in order to meet the increasing need of knock out cell line engineering of GOI, in particular, difficult-to-transfect cancer cell lines.
Applications for these stable cell lines include but not limited to drug screening, gene expression regulations, functional assays, membrane protein antibody FACS screening, antibody immunization boosting, protein localization study by fusion with GFP and living cell imaging, and in vivo tumor xenograft models.
Assay Cell Line Services: Advantages working with GenScript CellPower™
- LentiCRISPR technology licensed from Broad Institute, Inc. Cambridge, Massachusetts.
- Track record success with global pharma and biotech companies.
- Both virus and non-virus (chemical or physical) based on options are available for gene transfer.
- Track record of handling difficult-to-transfect cell lines, with 100+ target cells.
- Experience in stably expressing membrane proteins, especially for multiple transmembrane proteins.
- High quality standards for mycoplasma control and detection, covering 180 species of mycoplasma.
- Guaranteed mRNA expression. Multiple functional assays for clone validation: FACS, WB, qPCR, IP and etc.
- Cost-effective, a saving of 30% or more (Lentivirus constitutive starting at $16,200 $11,339; Plasmid starting at $16,910 $11,175).
Assay Cell Line Development services by CellPower™
| Service Name (Cat. No) | Time line | Deliverables |
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| CellPower™ Gene overexpression (SC1394) |
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| CellPower™ Functional assays (SC1394) |
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| CellPower™ Inducible system (gene overexpression) (SC1394) |
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| CellPower™ Lentivirus packaging (SC1394) |
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Over-expression of GOI
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Figure 1. FACS/qPCR to validate the expression of GOI (Gene of interest)
Knock-down Expression of GOI
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Figure 2. Validation of knockdown efficiency via western blotting (left) and functional assay (right).
Inducible expression of GOI
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Figure 3. Q‐PCR (left) and western blotting (right) validation of Target A expression in HepG2 cells.
Infection test for lentivirus
Bright field  GFP  |
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Figure 4. Infection test for lentivirus
Workflow of Our CellPower™ Stable Cell Line Development Process
