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CellPower™ Cell Line Development

For Drug Discovery, Toxicity Testing and Basic Research

Assay Cell Line Development: Gene Over-Expression and Functional Assays

Recombinant stable cell lines are one of the widely used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest (GOI) is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the GOI together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell line offers reproducible results for assay studies.
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After selection, cells that have the expression cassette incorporated into the genome will be enriched, resulting in stable pool cells. By clonal selection from the stable pool cells, single clone cells are generated. Both stable pools and single clones are frequently used in research.

Recombinant stable cell line development at GenScript includes over-expression, inducible expressionI. In addition, we developed lentivirus based CRISPR/Cas (Lenti-CRISPR/Cas9) technologies and platforms, in order to meet the increasing need of knock out cell line engineering of GOI, in particular, difficult-to-transfect cancer cell lines.

Applications for these stable cell lines include but not limited to drug screening, gene expression regulations, functional assays, membrane protein antibody FACS screening, antibody immunization boosting, protein localization study by fusion with GFP and living cell imaging, and in vivo tumor xenograft models.

Assay Cell Line Services: Advantages working with GenScript CellPower™

LentiCRISPR technology licensed from Broad Institute, Inc. Cambridge, Massachusetts.
Track record success with global pharma and biotech companies.
Both virus and non-virus (chemical or physical) based on options are available for gene transfer.
Track record of handling difficult-to-transfect cell lines, with 100+ target cells.
Experience in stably expressing membrane proteins, especially for multiple transmembrane proteins.
High quality standards for mycoplasma control and detection, covering 180 species of mycoplasma.
Guaranteed mRNA expression. Multiple functional assays for clone validation: FACS, WB, qPCR, IP and etc.
Cost-effective, a saving of 30% or more (Lentivirus constitutive starting at $16,200 $11,339; Plasmid starting at $16,910 $11,175).

Assay Cell Line Development services by CellPower™

Assay Cell Line by CellPower™: Service Specs

Service Name (Cat. No)	Time line	Deliverables
CellPower™ Gene overexpression (SC1394)	Starting from 8 weeks (Stable pool) Starting from 14 weeks (Single clone)	Report of cell line test 2 cryovials of stable pool cells with 10^6 cells per vial. Report of cell clone generation Report of screening of cell clones Two single-cell-derived stable clones (1×10^6 cells/vial) CoA report
CellPower™ Functional assays (SC1394)	Starting from 16 weeks (Stable pool) Starting from 22 weeks (Single clone)	Report of cell line test 2 cryovials of stable pool cells with 10^6 cells per vial. Report of cell clone generation Report of screening of cell clones Report of assay test One single-cell-derived stable clone (1×10^6 cells/vial) COA report/assay protocol
CellPower™ Inducible system (gene overexpression) (SC1394)	Starting from 12 weeks (Stable pool) Starting from 18 weeks (Single clone)	Report of cell line test 2 cryovials of stable pool cells with 10^6 cells per vial. Report of cell clone generation Report of screening of cell clones One single-cell-derived stable clone (1×10^6 cells/vial) COA report
CellPower™ Lentivirus packaging (SC1394)	2 weeks in general	High titer up to 10⁷-10⁹ IFU/mL based on targets Research level 1-10 mL concentrated lentivirus Titer detection and mycoplasma test report COA report