High Throughput Gene to Antibody Production
High throughput (HTP) gene to antibody production provides the most cost effective option for small-scale recombinant antibodies. This popular service combines GenScript's expertise in high-throughput gene synthesis with a proprietary transient antibody expression technology to produce high quality recombinant antibodies for both therapeutic antibody drug candidates and reagent antibodies.
Express hundreds of recombinant antibodies at once, starting from gene synthesis
Fast delivery of supernatant or purified antibodies for your screening project
Examples of antibody sequences accepted: variable region sequenced from hybridoma, hits (Fab or scFv) selected from display library based method, sequences from single B cell and/or repertoire analysis via next generation sequencing (NGS), and many more. You provide us the sequences of the fragments and we take care of the rest. Deliverables can be supernatant or purified antibodies. There is no additional set-up fee, and the cost of gene synthesis is waived.
High Throughput Gene to Antibody Production: Features
Fast Delivery
13-18 BD from gene synthesis to delivery (standard package)
Flexible Options
Fabs, scFv, VHH, or other fragments fused with Fc
Cost Effective
One-stop solution with an Economy option available
High Throughput Gene to Antibody Production: Workflow
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
|---|---|---|---|---|---|---|
| Antibody Sequence (Minimum 4 samples) | Mammalian | 10 mL of supernatant (SC1945) | 13 Business Days |
|
Starting under $350 per antibody (including gene synthesis) |  |
| 100 µg (SC1796) | 18 Business Days |
|
Starting under $500 per antibody (including gene synthesis) | |||
| 1 mg (SC1797) | ||||||
| Customized (SC1946) | ||||||
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1945 includes Western blotting against the Fc tag of the antibody to determine expression.
- QC Data for SC1796 & SC1797 includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
|---|---|---|---|---|---|---|
| Antibody Sequence (Minimum 4 samples) | Mammalian | 10 mL of supernatant (SC1945E) | 22-25 Business Days |
|
Starting AT $254 per antibody (including gene synthesis) | |
| 100 µg (SC1796E) | 28-30 Business Days |
|
Starting at $390 per antibody (including gene synthesis) | |||
| 1 mg (SC1797E) | ||||||
| Customized (SC1946E) | ||||||
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1945E includes Western blotting against the Fc tag of the antibody to determine expression.
- QC Data for SC1796E & SC1797E includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
|---|---|---|---|---|---|---|
| Antibody Sequence (Minimum 12 samples) | Mammalian | 0.5-1 mL of supernatant (SC1973) | 10 Business Days |
|
Starting at $332 per antibody (including gene synthesis) | |
| 0.5-1 mL supernatant (SC1793E) | 23-25 Business Days |
|
Starting at $245 per per antibody (including gene synthesis) |
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1973 and SC1973E includes Western blotting against the Fc tag of the antibody to determine expression.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
HTP Gene to Antibody Service Applications
This popular HTP gene to antibody service is designed to help researchers scale quickly from promising leads to purified recombinant antibodies in a microscale format, to accelerate their preliminary drug screening process. Applications served include epitope binning, ligand competition binding studies, affinity measurements, primary and secondary screen assays, HTP biophysical characterization to assess developability, and more.
Promising leads from your HTP studies can be seamlessly integrated into the GenScript workflow for scale up transient recombinant antibody production or stable cell pool/cell line production, for milligram to gram-level expression, which can support all your in vitro and in vivo applications.
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Quotations and Ordering
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