High Throughput Gene to Antibody Production

High Throughput Antibody Production (HTP Gene to Antibody) Service Details

Starting Material	Expression	Amount	Timeline	Deliverables	Price
Antibody Sequence (Minimum 4 samples)	Mammalian	10 mL of supernatant (SC1945)	13 Business Days	10 mL of Crude Supernatant QC Data	Starting under $350 per antibody (including gene synthesis)
		100 µg (SC1796)	18 Business Days	1-step affinity purified antibody as specified QC data	Starting under $500 per antibody (including gene synthesis)
		1 mg (SC1797)
		Customized (SC1946)

• Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
• Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
• Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
• QC Data for SC1945 includes Western blotting against the Fc tag of the antibody to determine expression.
• QC Data for SC1796 & SC1797 includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
• Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
• Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
• If ≥85% (antibody #) are expressed and delivered, the whole project will be charged in full; If <85% (antibody #) are expressed and delivered, we will charge for the delivered antibodies, and only charge a basic setup fee for gene synthesis, cloning and expression evaluation of the antibody sequences that are failed to deliver.

High Throughput Antibody Production (HTP Gene to Antibody) Service Details

Starting Material	Expression	Amount	Timeline	Deliverables	Price
Antibody Sequence (Minimum 4 samples)	Mammalian	10 mL of supernatant (SC1945E)	22-25 Business Days	10 mL of Crude Supernatant QC Data	Starting AT $254 per antibody (including gene synthesis)
		100 µg (SC1796E)	28-30 Business Days	1-step affinity purified antibody as specified QC data	Starting at $390 per antibody (including gene synthesis)
		1 mg (SC1797E)
		Customized (SC1946E)

• Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
• Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
• Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
• QC Data for SC1945E includes Western blotting against the Fc tag of the antibody to determine expression.
• QC Data for SC1796E & SC1797E includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
• Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
• Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
• If ≥85% (antibody #) are expressed and delivered, the whole project will be charged in full; If <85% (antibody #) are expressed and delivered, we will charge for the delivered antibodies, and only charge a basic setup fee for gene synthesis, cloning and expression evaluation of the antibody sequences that are failed to deliver.

High Throughput Antibody Production (HTP Gene to Antibody) Service Details

Starting Material	Expression	Amount	Timeline	Deliverables	Price
Antibody Sequence (Minimum 12 samples)	Mammalian	0.5-1 mL of supernatant (SC1973)	10 Business Days	0.5-1 mL of Crude Supernatant QC Data	Starting at $332 per antibody (including gene synthesis)
		0.5-1 mL supernatant (SC1793E)	23-25 Business Days	0.5-1 mL crude supernatant QC data	Starting at $245 per per antibody (including gene synthesis)

• Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
• Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
• Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
• QC Data for SC1973 and SC1973E includes Western blotting against the Fc tag of the antibody to determine expression.
• Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
• Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or HD 293F) and appropriate expression volume corresponding to the actual yield.
• If ≥85% (antibody #) are expressed and delivered, the whole project will be charged in full; If <85% (antibody #) are expressed and delivered, we will charge for the delivered antibodies, and only charge a basic setup fee for gene synthesis, cloning and expression evaluation of the antibody sequences that are failed to deliver.

HTP Gene to Antibody Service Applications

This popular HTP gene to antibody service is designed to help researchers scale quickly from promising leads to purified recombinant antibodies in a microscale format, to accelerate their preliminary drug screening process. Applications served include epitope binning, ligand competition binding studies, affinity measurements, primary and secondary screen assays, HTP biophysical characterization to assess developability, and more.

Promising leads from your HTP studies can be seamlessly integrated into the GenScript workflow for scale up transient recombinant antibody production or stable cell pool/cell line production, for milligram to gram-level expression, which can support all your in vitro and in vivo applications.