NEW! Economy option and HTP expression in 24-well plate format now available!
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Optimizing Recombinant Antibody Expression
A white paper with technical advice on how to optimize your system for rAb expression.
High throughput (HTP) gene antibody production provides the most cost effective option for small-scale full-length recombinant antibodies, where you may choose either supernatant or purified antibodies (ug to mg level). From customer-supplied antibody sequences to final deliverables, HTP antibody production projects take as short as 18 business days (standard packages)! There is no additional set-up fee, and the cost of gene synthesis is waived.
This popular service combines GenScript's expertise in high-throughput gene synthesis with a proprietary transient antibody expression technology to produce high quality recombinant antibodies for both therapeutic antibody drug candidates and reagent antibodies.
Examples of antibody sequences accepted: variable region sequenced from hybridoma, hits (Fab or scFv) selected from display library based method, sequences from single B cell and/or repertoire analysis via next generation sequencing (NGS), and many more.
HTP Gene to Antibody Production Features
Fastest Turnaround Time in the Industry
Express delivery of your antibodies in just 18 business days*!* For standard package
Flexible Options for Testing Recombinant Antibodies
Multiple formats (Fc.fusion fragments) for expression; Flexible optional QCs. Now available in 24-well expression format! New!
Cost Effective Option for your Investment
One-stop solution: From gene synthesis to purified recombinant antibody. An Economy option is available! New!
HTP Gene to Antibody Production
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
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| Antibody Sequence (Minimum 4 samples) | Mammalian | 10mL of supernatant (SC1945) | 13 Business Days |
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Starting under $350 per antibody (including gene synthesis) |  |
| 100µg (SC1796) | 18 Business Days |
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Starting under $500 per antibody (including gene synthesis) | |||
| 1mg (SC1797) | ||||||
| Customized (SC1946) | ||||||
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, and mouse IgG2a; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1946 includes Western blotting against the Fc tag of the antibody to determine expression.
- QC Data for SC1796 & SC1797 includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or Expi-293) and appropriate expression volume corresponding to the actual yield.
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
|---|---|---|---|---|---|---|
| Antibody Sequence (Minimum 4 samples) | Mammalian | 10mL of supernatant (SC1945E) | 22-25 Business Days |
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Starting AT $254 per antibody (including gene synthesis) | |
| 100µg (SC1796E) | 28-30 Business Days |
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Starting at $390 per antibody (including gene synthesis) | |||
| 1mg (SC1797E) | ||||||
| Customized (SC1946E) | ||||||
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, and mouse IgG2a; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1946E includes Western blotting against the Fc tag of the antibody to determine expression.
- QC Data for SC1796E & SC1797E includes A280 for Antibody concentration and SDS-PAGE for purity assessment.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or Expi-293) and appropriate expression volume corresponding to the actual yield.
High Throughput Antibody Production (HTP Gene to Antibody) Service Details
| Starting Material | Expression | Amount | Timeline | Deliverables | Price | |
|---|---|---|---|---|---|---|
| Antibody Sequence (Minimum 12 samples) | Mammalian | 0.5-1 mL of supernatant (SC1973) | 10 Business Days |
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Starting at $332 per antibody (including gene synthesis) | |
| 0.5-1 mL supernatant (SC1793E) | 23-25 Business Days |
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Starting at $245 per per antibody (including gene synthesis) |
- Project can begin from HTP gene synthesis or customer-supplied DNA but Antibody sequences are required for project evaluation in both cases.
- Turnaround time does NOT include shipping details. Please add approximately 3-5 days to account for shipping onto the dates listed.
- Pricing applicable to human IgG1, human IgG4, and mouse IgG2a; Kappa (ĸ)/lambda(Λ) light chains.
- QC Data for SC1973 and SC1973E includes Western blotting against the Fc tag of the antibody to determine expression, and SDS-PAGE for purity assessment.
- Additional QC such as SEC-HPLC, HTP ELISA, Endotoxin Testing, LC-MS, and peptide mapping available upon request (pricing may vary).
- Based on the specific antibodies clients request, we will choose the proper expression cells (either HEK293 or Expi-293) and appropriate expression volume corresponding to the actual yield.
HTP Gene to Antibody Service Applications
This popular HTP gene to antibody service is designed to help researchers scale quickly from promising leads to purified recombinant antibodies in a microscale format, to accelerate their preliminary drug screening process. Applications served include epitope binning, ligand competition binding studies, affinity measurements, primary and secondary screen assays, HTP biophysical characterization to assess developability, and more.
Promising leads from your HTP studies can be seamlessly integrated into the GenScript workflow for scale up transient recombinant antibody production or stable cell pool/cell line production, for milligram to gram-level expression, which can support all your in vitro and in vivo applications.
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Recombinant Antibody Production-Related Services
Not what you are looking for?
- Need more antibodies? Check out MamPilot™ guaranteed antibody expression package, get your purified antibodies just in 4 weeks.
- Still unsure? Use this service guide to determine which is the best match for your application.
Discover the benefits of recombinant antibodies »
Quotations and Ordering
Our customer service repsentatives are available 24 hours a day, Monday through Friday, to assist you.
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Order by email: protein@genscript.com
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Phone: 1-877-436-72 (Toll-Free); 1-732-885-9188
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Fax: 1-732-210-0262 1-732-885-5878
