
Optimized workflow and robust QC ensures similar viral (IFU) and functional titers (TU)

Viral Titer (108)
Gene Type | Titer | Volume | QC | Application | Turnaround Time | Price |
---|---|---|---|---|---|---|
sgRNA | >10^6 TU/mL | 200µL Supernatant |
|
Cell Culture |
100-200 virus: 3-4 wks 200-400 virus: 4-5 wks |
Get A Quote |
GOI: < 1,500 bp | ||||||
GOI: 1,501-3,000 bp | ||||||
GOI: 3,000 - 5,000 bp | 100-200 virus: 5-6 wks 200-400 virus: 6-7 wks |
Note:
1. Price and TAT including gene synthesis, plasmid prep and lentivirus packaging
2. TAT only guaranteed when using GenScript’s backbone
3. Our lentiviral vector packaging service is only suitable for in vitro applications
4. For sgRNA and GOI < 3 kb, we guarantee 90% of sample reach to titer of 10^6 TU/mL
Optimized workflow and robust QC ensures similar viral (IFU) and functional titers (TU)
Viral Titer (108)
Add-On Service | Description | |
---|---|---|
1 | FACS analysis | Validate the transgene expression by FACS analysis. A validated antibody with specific binding to target protein or reporter tag must be provided. |
2 | Sanger Sequencing | Validate the integrity of inserted gene of interest (GOI). |
Vector Name | V1 | V2 | V3 | V4 | V5 | V6 | V7 | V8 | V9 | V10 | V11 | V12 | V13 | V14 | V15 | V16 | V17 | V18 | V19 | V20 | PC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GOI Length | 1056 | 2634 | 2631 | 2196 | 2196 | 741 | 318 | 174 | 5304 | 3133 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
Physical Titer * 10^6 TU/mL |
8.15 | 5.57 | 4.55 | 12.25 | 9.14 | 10.73 | 6.77 | 11.23 | 5.26 | 1.56 | 10.34 | 3.73 | 1.64 | 1.64 | 13.82 | 13.27 | 14.28 | 24.6 | 26.1 | 25.3 | 13.91 |
Functional Titer * 10^6 TU/mL |
1.5 | 1.25 | 0.5 | 2.5 | 1.25 | 2.5 | 2.5 | 2.5 | 0.85 | 0.25 | 2.5 | 0.63 | 1.02 | 1.17 | 1.25 | 1.25 | 2.5 | 15.6 | 17.3 | 16.1 | 4 |
> 10^6 TU/mL | 100% |
---|---|
> 10^7 TU/mL | 50% |
> 10^6 TU/mL | 85% |
---|---|
< 10^6 TU/mL | 15% |
Physical Titer
Sample | Backbone | Titer | Volume | TAT |
---|---|---|---|---|
96 lentivirus | LentiCRISPR V2 | >10^7 | 200ul | 3 wks |
Transduction Test
293T, GFP fluorescence, MOI:30
GenScript offers proprietray lentiviral packaging platform to ensure you getting functional intact virus everytime.
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