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Homology-Directed Repair (HDR) Knock-in Templates


One powerful application of CRISPR/Cas genome editing technology involves the precise insertion of DNA sequences via the homology-directed repair (HDR) pathway. While many factors may affect the efficacy of this approach, choosing the right HDR template is certainly an important one. GenScript now offers high quality, sequence verified HDR templates for maximizing the editing efficiency of your CRISPR experiments.

CRISPR HDR Knock-in Templates

Resources

─ Highly efficient non-viral T cell engineering process at clinical scale with GenExact™ ssDNA

Collaboration with Marson's lab from UCSF (University of California San Francisco) showed that:

Click to view the PDF version

Brian R Shy. et.al., bioRxiv(2021), Read the whole article.

─ Case Study

Long hybrid ssDNA HDR templates enable high yield non-viral cell therapy manufacturing

In collaboration with the Marson lab at the Gladstone-UCSF Institute of Genomic Immunology

Download Case Study

New Case Study

Long hybrid ssDNA HDR templates enable
high yield non-viral cell therapy manufacturing

In collaboration with the Marson lab at the Gladstone-UCSF Institute of Genomic Immunology

Free Download

─ GenWand™ dsDNA outperforms PCR products in knocking in larger genes

GFP KI at RAB11A Locus in HEK293 cells using electroporation with 5ug of PCR dsDNA and GenWandTM dsDNA templates.

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