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Homology-Directed Repair (HDR) Knock-in Templates


One powerful application of CRISPR/Cas genome editing technology involves the precise insertion DNA sequences via the homology-directed repair (HDR) pathway. While many factors may affect the efficacy of this approach, choosing the right HDR template is certainly an important one. GenScript now offers high quality, sequence verified HDR templates for maximizing the editing efficiency of your CRISPR experiments.

CRISPR HDR Knock-in Templates

Resources

─ Highly efficient non-viral T cell engineering process at clinical scale with GenExact™ ssDNA

Collaboration with Marson's lab from UCSF (University of California San Francisco) showed that:

Click to view the PDF version

Brian R Shy. et.al., bioRxiv(2021), Read the whole article.

─ GenWand™ dsDNA outperforms PCR products in KI efficiency and toxicity

GFP KI at TRAC Locus using electroporation with 2 µg of dsDNA templates

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