Overview

One powerful application of CRISPR/Cas genome editing technology involves the precise insertion of DNA sequences via the homology-directed repair (HDR) pathway. While many factors may affect the efficacy of this approach, choosing the right HDR template is certainly an important one. GenScript now offers high quality, sequence verified HDR templates in both linear and circle format for maximizing the editing efficiency of your CRISPR experiments, and efficiently support non-viral cell therapy development .

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CRISPR HDR Knock-in Templates

GenExact™ ssDNA
Precise KI and low cytotoxicity

Application

Ideal for T cell therapy

Ideal for cell/animal model generation

Advantage

Minimal cytotoxicity

Precise knock-in, minimized off-target effect

High purity and sequence verified

Specifications

Insertion length: 150-5,000 nt

µg to mg scale

Research to cGMP grade

GenWand™ dsDNA
Long gene knock-in in large scale

Application

Ideal for long gene CRISPR KI

Ideal for screening and scale up

Advantage

Covalently closed ends protection for better accuracy

More suitable for scale up

High purity and sequence verified

Specifications

Insertion length: 2-10 kb

µg to g scale

Research to cGMP grade

GenCircle™ dsDNA
Circular template with no resistance gene

Application

Ideal replacement for plasmid HDR template

Ideal for scale up and fast delievery

Advantage

KI efficiency increase by up to 30%

429bp backbone, lower cytotoxicity & higher transfection efficiency

No antibiotic resistance gene to avoid regulatory concern

High purity and sequence verified

Specifications

Insertion length: 1-50 kb

µg to g scale

cGMP grade coming soon

Resources

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with GenScript CRISPR Experts

Get in Touch with GenScript CRISPR Experts
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