Immunopeptidome Profiling for Neoantigen Candidate Discovery
Neoantigen identification is a major bottleneck in the development of personalized cancer immunotherapies. Currently, GenScript offers reliable neoantigen peptide production for neoantigen candidate screening. Now, in partnership with Cayman Chemical, GenScript will now also offer a unique Immunopeptidome Profiling service for neoantigen candidate discovery.
What Is Immunopeptidome Profiling?
Immunopeptidome profiling is a deep sequence analysis of MHC-associated peptides to help clients identify neoantigens and potential immunogenic sequences.
Human or mouse tumor cell are lysed under optimal conditions for releasing intact MHC complexes. Using immunoaffinity chromatography, MHC-specific antibodies enrich the pool of MHC class I or class II from lysates. MHC associated peptides are released under gentle eluting conditions and separated from the captured MHC molecules. The peptides are sequenced by LC-MS/MS and then matched to protein sequence databases or RNA-seq data, as well as analyzed for mutations and post-translational modifications to facilitate the identification of potential neoantigens.
Benefits of Cayman Chemical’s Immunopeptidome Service
Identification of thousands of MHC-associated peptides with Optimized workflows for MHC class I and II (human or animal)
Large-scale cell culture and transfection capabilities to suit your unique study with workflows adaptable to cells or tumor tissue
Analysis using Orbitrap MS and unbiased, deep sequencing of the MHC peptidome
Are You Interested in Learning More About Cayman Chemical’s Immunopeptidome Profiling Service? Click Here To Fill Out Your interest Form Today!
Learn More About Cayman Chemical’s Immunopeptidome Service at https://www.caymanchem.com/services/bioanalysis/immunopeptidome