A Novel Class of Functional RNA Molecules

GenScript proudly offers custom circular RNA (circRNA) synthesis via our proprietary circRNA IVT manufacturing service.

circRNA is a single-stranded RNA in which the 5’ and 3’ ends join to form a covalently closed loop. The circular structure confers multiple functional benefits, including improved stability and longer expression duration.

Custom circRNA synthesis is available now for 100bp – 4 Kb insert sequences with delivery in only 3 weeks.

Benefits of circRNA

Proprietary circRNA vector and purification method, leading to high circularization efficiency and purity

Improved stability & increased and longer protein expression duration vs linear mRNA

Lower immunogenicity vs Cap1 linear mRNA


 circrna workflow

GenScript circRNA offers the flexibility to meet your project needs


Send Us Your ORF or IRES

We will design your circRNA expression vector with T7 promoter, PIE, IRES and ORF

Gene Synthesis to Plasmid Prep

Our integrated workflow will take care of all the prerequisites to produce your circRNA

RUO Grade IVT Production

µg to mg customized RUO circRNA service with advanced purification (RP-HPLC) and QC options

Quality Control and Specification

QC Item Test Method Specification RUO
Identification Appearance Visual Inspect Clear and free of foreign particles
RNA Length Agarose Gel Electrophoresis Expected size band detected​
Identification RNaseR degradation test​ Not be degraded by RNaseR
RNA Content​ UV Absorbance​ Target ± 5%​
PH pH meter Target ± 0.5
Buffer Specification Client Spec N/A
Purity A 260/280 Ratio UV Spec 1.70 ~ 2.30
Purity by HPLC HPLC ≥80 %​
Impurity Total protein residue Nano Orange Assay ≤ 1%
Plasmid DNA Residue qPCR ≤ 0.1%​
Safety Endotoxin Semiquantitative < 10EU/mg
Endotoxin Quantitative < 10EU/mg
Bioburden Sdirect Inoculation No Growth after
48 hrs

Case Studies



Circular RNA Case Study Report
Circular RNA Case Study Report

Circular RNA introduction, GenScript offering details, and case studies showing increased stability, prolonged expression, and lower immunogenicity compared to linear IVT mRNA.

Free Download

Additional mRNA Offerings

Get in Touch
with GenScript Custom Circular
RNA Synthesis

Get in Touch with GenScript IVT mRNA Production Services
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