One Stop Solutions for IVT RNA

In vitro transcribed RNA has many applications in research and biotechnology, including gene expression studies, RNA-based therapeutics, RNA vaccines, and diagnostics.

GenScript’s full-service solution to IVT RNA manufacturing streamlines your workflow starting from gene synthesis to IVT linear mRNA, circular RNA, and self-amplifying RNA synthesis to LNP formulation. RNAs produced at GenScript are optimized with our proprietary production platform, ensuring the quality and expression efficiency.

Highlights

Why GenScript

Integrated Process,
Comprehensive Solutions

  • From gene to IVT mRNA/circRNA/saRNA
    to LNP
  • Off-the-shelf and fully customized

Proprietary
Vector

  • Optimized mRNA vectors with optimized UTRs and built-in Poly(A)
  • Proprietary vectors for circRNA and saRNA production

Purification &
QC Flexibility

  • Proprietary purification process
  • Oligo dT/dsRNA removal and QC
    analysis available

Testimonials

Dr. Sang-Moo Kang Dr. Sang-Moo Kang

Institute for Biomedical Sciences at Georgia State University

GenScript mRNA has helped us make a rapid progress in developing novel vaccine projects. GenScript service is faithful and timely provided, saving me lots of time to focus on research. This is why I have been using GenScript services for over 20 years.

Comparison of different IVT RNA payload formats

Linear mRNA Self-Amplifying RNA Circular RNA
Structure Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Close loop; No free 5’ 3’ end
Eliminating mRNA exonuclease degradation
Ribosome recruiting 5’ Cap:
Initiates translation & stabilizes mRNA
5’ Cap:
Initiates translation & stabilizes mRNA
Internal Ribosome Entry Site (IRES) :
Initiates translation
ORF
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M1Ψ
  • Insert < 20 kb
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M5C
  • Insert < 6 kb
  • Codon optimization for translation yield
  • Insert < 4 kb
UTR Regulatory elements in the 5’ and 3’-UTR stabilize mRNA and increase translation initiation Replicon for further self-replication of the mRNA Include IRES + PIE + other elements
PolyA tail Stabilizes RNA molecule & Prevents degradation Stabilizes RNA molecule & Prevents degradation Not required
Protein Expression +++ Strong +++++ Strongest ++++ Stronger
Expression half-life +++ Shorter +++++ Longest ++ Longer
Immunogenicity ++++ Low ++ High +++ Medium
Dosage requirement ++ High +++++ Lowest +++ Medium
Novelty +++ Traditional ++++ Novel +++++ Novel

Resources

标题
mRNA Case Study Report

Case studies showing high expression efficiency of GenScript’s mRNA for expressing SB transposase, GFP, Luciferase, eSpCas9, and more.

Free Download
Circular RNA Case Study Report
Circular RNA Case Study Report

Circular RNA introduction, GenScript offering details, and case studies showing increased stability, prolonged expression, and lower immunogenicity compared to linear IVT mRNA.

Free Download
LNP Case Study Report
LNP Case Study Report

Case studies demonstrating encapsulation and delivery efficiency of GenScript’s new LNP formulations in cell models, and mouse models via both IM and IV.

Free Download
Critical Consideration in Sourcing mRNA_Whitepaper
Critical Consideration in Sourcing mRNA_Whitepaper

This whitepaper provides valuable insights into the essential factors that vaccine and therapy developers should consider when exploring mRNA solutions

Free Download
Advancements in mRNA manufacturing_eBook
Advancements in mRNA manufacturing_eBook

Get GenScript’s free application eBook on therapeutics development with mRNA manufacturing solutions

Free Download
mRNA Therapy Modalities_Infographic
mRNA Therapy Modalities_Infographic

Learn about key structural features and functions of IVT mRNA and LNP, therapeutic modalities beyond prophylactic vaccines, mRNA candidates progressing through clinical trials.

Free Download
mRNA Parts_Infographic
mRNA Parts_Infographic

Learn about the main structural components of mRNA, the functional roles of each part, what constitutes high-quality IVT mRNA and more.

Free Download

REQUEST A QUOTE

feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*