Monkeypox Virus Real-time PCR (RTPCR) Test

The kit is based on RT-PCR technology and PCR amplification in one-step. The assay amplifies the conserved gene of the Monkeypox viral DNA where this targeted region is amplified and detected by designed primers and fluorescence probes. An additional primer/probe set is used to detect the human gene (RNase P gene) for Internal Control (IC). The IC is used to ensure proper execution of the assay by the end user, including sample collection, nucleic acid extraction and the PCR process.