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GST Fusion Protein Purification Kit
GST Fusion Protein Purification Kit
| Description | GenScript Glutathione Resin (L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. The GST Fusion Protein Purification Kit(L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification. GenScript Protein Expression and Purification Kit (L00208) provide all the major reagents and materials for the cloning, expression and purification of target protein. The target protein is first expressed as a GST fusion protein in E. coli using the GenScript expression vector pGS-21a. The GST fusion protein is bound to a column packed with GenScript Glutathione Resin (L00206) and then eluted with reduced glutathione. Finally, to obtain the tag-free target protein, the purified GST fusion protein is digested with recombinant porcine Enterokinase (EK) to remove GST tag. Storage:Store at 4°C,do NOT freeze. |
| Key Features | Key Features:
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| Storage | Store at 4°C |
Habyarimana F, et al. The sdiA-regulated gene, srgE, encodes a Type 3 secreted effector. J Bacteriol. 2014April;
Yuan B, et al. A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice. Am J Pathol. 2014July;184(7):1967-80
Habyarimana F, et al. The sdiA-regulated gene, srgE, encodes a Type 3 secreted effector. J Bacteriol. 2014April;
Bobba S., et al. Microtiter plate-based assay for inhibitors of penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011 Jun;55(6):2783-7.
Ali MF., et al.Golgi phosphoprotein 3 determines cell binding properties under dynamic flow by controlling Golgi localization of core 2 N-acetylglucosaminyltransferase 1.J Biol Chem. 2012 Nov 16;287(47):39564-77.
Petrosyan A., et al. Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail. Int J Biochem Cell Biol. 2012 Jul;44(7):1153-65.
You can visit "Citations Database" for more citations.
Yuan B, et al. A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice. Am J Pathol. 2014July;184(7):1967-80
Habyarimana F, et al. The sdiA-regulated gene, srgE, encodes a Type 3 secreted effector. J Bacteriol. 2014April;
Bobba S., et al. Microtiter plate-based assay for inhibitors of penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011 Jun;55(6):2783-7.
Ali MF., et al.Golgi phosphoprotein 3 determines cell binding properties under dynamic flow by controlling Golgi localization of core 2 N-acetylglucosaminyltransferase 1.J Biol Chem. 2012 Nov 16;287(47):39564-77.
Petrosyan A., et al. Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail. Int J Biochem Cell Biol. 2012 Jul;44(7):1153-65.
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. L00207 | ||
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Size |
Price |
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| 1 kit | $139.00 | |
