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Catalog Products » Protein Analysis & Purification » Protein Isolation and Purification » Protein Purification Resins » Protein G Resin

Protein G Resin

Description GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. Protein G Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg goat IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00209Protein G Resin (5ml)10 ml50% slurry

Storage:Store at 4°C, do NOTfreeze.
Key Features
Key Features
  • Broad IgG binding spectrum
  • Binding specificity complements of protein A
  • Agarose media
  • No specific albumin binding
  • Optimized homogeneous recombinant ligand
  • High capacity
  • Available in bulk size

Specifications
LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitution Approximately 2 mg protein G/ml settled resin
Static binding capacity >20 mg goat IgG/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months if store unopened at 2-8℃


Storage Store at 2-8°C, do NOT freeze.


Cat. No. Product Name Quantity Price
Z02007
Protein G - 5 mg
$95.00
 
Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014Aug.;

Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014Aug.;

L Feng, et al. Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. Dev Biol. 2014Sep.;

P Zhao, et al. Generation and Characterization of Polyclonal Antibody Against Part of Immunoglobulin Constant Heavy υ Chain of Goose. Monoclon Antib Immunodiagn Immunother. 2014Aug.;33(4):287-90

Zhang Y, et al. Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014Aug.;

N Zilio, et al. A Novel HDAC Complex in the Control of Transcription and Genome Stability. Mol Cell Biol. 2014July;

Kan W, et al. M3 Muscarinic Receptor Interaction with Phospholipase C β3 Determines its Signaling Efficiency. J Biol Chem. 2014Mar.;

CW Armitage, et al. Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens. Immunol Cell Biol. 2014Jan.;

Short KR, et al. Antibodies mediate formation of neutrophil extracellular traps in the middle ear and facilitate secondary pneumococcal otitis media. Infect Immun. 2014Jan.;82(1):364-70

Muth S, et al. A CD40/CD40L feedback loop drives the breakdown of CD8 T‐cell tolerance following depletion of suppressive CD4 T cells. Eur J Immunol. 2014Jan.;

Armistead JS., et al.Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of falciparum and vivax malaria. Infect Immun.2013 Dec 9.

Wiriya Chiranand, et al. The CTA4 Transcription Factor Mediates Induction of Nitrosative Stress Response in Candida albicans. Eukaryot Cell. 2008 Feb;7(2):268-78.

Matthew Staron, et al. Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex. Blood. 2011 Jun 30;117(26):7136-44.

Bikkavilli RK., et al. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. J Cell Sci. 2012 May 15;125(Pt 10):2446-56.

Muth S., et al. Release Of Dendritic Cells From Cognate Cd4 T-Cell Recognition Results In Impaired Peripheral Tolerance And Fatal Cytotoxic T-Cell Mediated Autoimmunity. Proc Natl Acad Sci. 2012 Jun 5;109(23):9059-64.

Tubon TC Jr., et al. dCREB2-Mediated Enhancement of Memory Formation. J Neurosci. 2013 Apr 24;33(17):7475-87.

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Cat. No. L00209
Size Price
5 ml $129.00