The TUNEL Universal Apoptosis Detection Kit (Biotin-labeled POD) (Cat. No. L00290) is one of GenScript’s newly introduced products. It is used for assaying adherent Cells, paraffin-embedded tissue sections and cryopreserved tissue sections. The Kit could detect the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-Diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
|Storage||Store Streptavidin-HRP, DAB-A Solution, DAB-B Solution, DAB-C Solution at 4°C, and do not expose to light. Store the rest of the kit at -20°C. It will remain stable for one year.|
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