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TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)

*This product has been discontinued!*
TUNEL Apoptosis Detection Kit
L00296
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Description


Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (Biotin-labeled POD)(Cat. No. L00296) is one of GenScript’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3,3’-Diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Product Introduction
Key Features
Key Feature
  • Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.
Storage Store streptavidin-HRP, DAB-A Solution, DAB-B Solution, DAB-C Solution at 4°C, and do not expose it to light. Store the rest of the kit at -20°C. It will remain stable for one year.
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