Catalog Products » GenCRISPR™ Cas9 Genome Editing Products » GenCRISPR™ Nucleases » GenCrispr Mutation Detection Kit

GenCrispr Mutation Detection Kit

GenCrispr Mutation Detection Kit
L00688
$180.00

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Description

The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.

Components Amount provided
25-reaction kit 100-reaction kit
High-Fidelity DNA polymerase 10 μL 40 μL
5X PCR Reaction Buffer 150 μL 600 μL
GenCrispr T7 Endonuclease I (Cat.No.Z03396) 25 μL 100 μL
10X GenCrispr T7 Endonuclease I Reaction buffer 50 μL 500 μL
Control Template DNA 30 μL 120 μL
Control Primer Mix 30 μL 120 μL
Protease K 25 μL 100 μL
dNTP 15 μL 60 μL

Note

Research purposes only.

Product Introduction
Storage Store all components at -20°C upon receipt.

Applications
Detect the gene mutagenesis and SNP, for cleavage efficiency assay caused by ZFNs, TALENs, CRISPR/Cas9 or other gene editing tools.

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