|Description||The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
|Storage||Store all components at -20°C upon receipt.|
|Note||Research purposes only.|
|Detect the gene mutagenesis and SNP, for cleavage efficiency assay caused by ZFNs, TALENs, CRISPR/Cas9 or other gene editing tools.
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