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Catalog Products » Other Products » GenCrispr sgRNA Screening Kit

GenCrispr sgRNA Screening Kit


Description Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.

Components Amount
30-reaction kit 100-reaction kit
GenCrispr Cas9 Nuclease 15 μL 50 μL
10X Reaction Buffer 100 μL 300 μL
Positive Control sgRNA 3 μg 10 μg
Positive Control Substrate 60 μL 200 μL
RNase-free water 1 mL 1 mL
Storage Store at -20°C upon receipt. positive control sgRNA should be aliquoted and stored at -80°C, to avoid repeated freeze/thaw cycles.
GenCrispr SgRNA Screening Kit
GenCrispr SgRNA Screening Kit

gRNA efficiency screening using GenCRISPR sgRNA screening kit.
Twelve unique synthesized gRNAs were incubated with Cas9 nuclease for 1 h at 37°C according to the instructions in theGenCRISPR sgRNA screening kit manual. Upon separating the reactions by agarose gel electrophoresis, only gRNA9 was observed to induce cleavage.




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Cat. No. L00689
Size
Price
30 reactions $166.00
100 reactions $400.00
 
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