|Description||The High-Efficiency gRNA-Cas9-Puro plasmid Assembly Kit is used for easier and highly efficient cloning of gRNA fragments into Cas9 vectors. This vector has been constructed by integrating a suicide reporter gene into the gRNA insert site. Under the selection of this suicide report gene, almost 100% of clones are positive clones without screening. The High-Efficiency gRNA-Cas9-Puro plasmid is provided in both the circular and linear formats. The Puromycin resistant gene is linked by 2A, a self-cleavage peptide, and is expressed separately from Cas9, keeping the maximum activity of Cas9.|
|Storage||Store at -20°C upon receipt.|
|Note||Research purposes only.|
|Rapid construction of gRNA-Cas9 plasmid for the subsequent gene editing research.
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