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Control Antibodies

Isotype Control Antibodies

The selection of an appropriate isotype control is key for flow cytometry experiments. Isotype control antibodies that have no relevant specificity can distinguish non-specific "background" staining from specific antibody staining. Three main factors contribute to the levels of background staining associated with a primary antibody: a) binding to Fc receptors on target cells, b) non-specific protein interactions with cellular proteins, lipids, or carbohydrates, and c) cell autofluorescence. All of these factors vary greatly depending on the target cell type and the isotype of the primary antibody. Therefore, in order to accurately determine the level of specific staining by the primary antibody, isotype controls need to be matched to the specific primary antibodies being used. GenScript offers a series of isotype control antibodies that can be used in your research.

Loading Control Antibodies

Loading control proteins are known to be constitutively and stably expressed at high levels in almost all tissues and cells. Housekeeping proteins, such as GAPDH, tubulin, and actin, represent some of the well-known loading control proteins.

Loading control antibodies are used in Western blotting for two main purposes:

1. To estimate relative amounts of total protein loaded in each lane. (This information is needed to determine if the difference in levels of the protein of interest in different samples is due to loading and transfer errors or to real changes in protein expression.)
2. To troubleshoot the Western blotting procedure.

GenScript offers a wide selection of polyclonal and monoclonal loading control antibodies for GAPDH, α-tubulin, and β-actin for several species. HRP- and biotin-conjugated versions of these antibodies are also available.

• Product Details
• Examples

Isotype Control Antibodies

	Cat. No.	Product Name	Price
	A01010	Chicken IgY Control (Whole Molecule), Purified - 4 mg	$69.00
	A01009	Goat IgG Control (Whole Molecule), Purified - 4 mg	$69.00
	A01383	Human IgG Control [FITC] (Whole Molecule), Purified - 0.5 mg	$69.00
	A01006	Human IgG Control(Whole Molecule), Purified - 4 mg	$69.00
	A01007	Mouse IgG control (Whole Molecule), Purified - 1 mg	$69.00
	A01381	Mouse IgG Control [FITC] (Whole Molecule), Purified - 0.5 mg	$69.00
	A01008	Rabbit IgG Control (Whole Molecule), Purified - 4 mg	$69.00
	A01730	Mouse IgG2b control (Whole Molecule), Purified - 1 mg	$69.00
	A01382	Rabbit IgG Control [FITC] (Whole Molecule), Purified - 0.5 mg	$69.00

 

Loading Control Antibodies

	Cat. No.	Product Name	Price
	A01865-40	MonoRabᵀᴹ Beta Actin Antibody, mAb, Rabbit - 40 µg	$198.00
	A00702-40	THETM beta Actin Antibody, mAb, Mouse - 40 ug	$69.00
	A00702-200	THETM beta Actin Antibody, mAb, Mouse - 200 ug	$311.00
	A00702-100	THETM beta Actin Antibody, mAb, Mouse - 100 ug	$175.00
	A00730-200	THETM beta Actin Antibody [HRP], mAb, Mouse - 200 ug	$333.00
	A00730-100	THETM beta Actin Antibody [HRP], mAb, Mouse - 100 ug	$180.00
	A01546	THETM beta Actin Antibody [Biotin], mAb, Mouse - 100 ug	$180.00
	A01410-40	THETM alpha Tubulin Antibody, mAb, Mouse - 40 ug	$108.00
	A01410-100	THETM alpha Tubulin Antibody, mAb, Mouse - 100 ug	$225.00
	A01490	THETM alpha Tubulin Antibody [HRP], mAb, Mouse - 100 ug	$335.00
	A01545	THETM alpha Tubulin Antibody [Biotin], mAb, Mouse - 100 ug	$335.00
	A00885-40	α-Actin-1 Antibody, pAb, Rabbit - 40 ug	$99.00
	A00885-200	α-Actin-1 Antibody, pAb, Rabbit - 200 ug	$349.00
	A01203-40	β-Tubulin III Antibody, pAb, Rabbit - 40 ug	$99.00
	A01622-40	GAPDH Antibody, mAb, Mouse - 40 ug	$99.00
	A00191-40	GAPDH Antibody, pAb, Goat - 40 ug	$99.00
	A00192-40	GAPDH Antibody [HRP], pAb, Goat - 40 ug	$99.00
	A00915-40	GAPDH Antibody [Biotin], pAb, Goat - 40 ug	$99.00
	A01419-40	VDAC1 Antibody, pAb, Rabbit - 40 ug	$99.00
	A01717-40	β-tubulin Antibody (2G7D4), mAb, Mouse - 40 ug	$99.00

 

Examples:

1. Sensitivity Comparison

Primary Antibody:

• 0.1 μg/ml THE™ β-Actin mAb(Mouse)(GenScript, A00702)
• 0.1 μg/ml β-Actin mAb (Mouse) (Company X)

Secondary Antibody:

• Goat Anti-Mouse IgG (H&L) [HRP] pAb (GenScript, 1:10,000, A00160)
• The signal was developed with Lumisensor™ HRP Substrate Kit (GenScript, L00221V500)

2. Immunofluenrescence Analysis

Primary Antibody:

• 0.1 μg/ml THE™ β-Tubulin mAb (GenScript, A01410)

Secondary Antibody:

• Fluorescein conjugated affinity purified anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)

3. Fluorescence Western Blotting

Primary Antibody:

• 0.1 μg/ml GAPDH Antibody, pAb, Goat (GenScript, A00191)

Lane 1-8:

• 5.0, 2.5,1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 μg of Hela cell lysate
• The signal was developed with One-Step Western™ Fluorescent Kit (GenScript, L00397)

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