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Biotin-Protein A
Biotin-Protein A
| Description | Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. |
| Formulation | Liquid, 2mg/ml in PBS (pH7.4) containing 10mg/ ml BSA ans 0.02% sodium azide. |
| Storage | Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use. |
| Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized. |
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
M00089 |
HRP-Protein A - 1 mg |
$55.00 |
|
M00090 |
HRP-Protein G - 1 mg |
$75.00 |
|
M00091 |
Streptavidin-HRP - 1 mg |
$89.00 |
|
M00097 |
Biotin-Protein L - 1 mg |
$98.00 |
|
M00098 |
HRP-Protein L - 1 mg |
$95.00 |
For more documents, please visit "Technical Support".
| Cat. No. M00095 | ||
|---|---|---|
| Size | Price | |
| 2 mg | $80.00 | |
