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Catalog Products » Protein Analysis & Purification » Protein Detection and Immunoassay » Reagents for Protein Analysis » Biotin-Protein L

Biotin-Protein L

Description Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
Storage The product is stable in lyophilized form if stored at -20°C or below. The reconstituted product can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Dot blot: 1:2,000-1:20,000
Western blot: 1:2,000-1:20,000
ELISA: 1:20,000-1:100,000
Other: user-optimized

Cat. No. Product Name Quantity Price
HRP-Protein A - 1 mg
HRP-Protein G - 1 mg
Streptavidin-HRP - 1 mg
Biotin-Protein A - 2 mg
HRP-Protein L - 1 mg
Lanitis E., et al. Redirected antitumor activity of primary human lymphocytes transduced with a fully human anti-mesothelin chimeric receptor. Mol Ther. 2012 Mar;20(3):633-43.

Morgan RA., et al. Recognition of glioma stem cells by genetically modified T cells targeting EGFRvIII and development of adoptive cell therapy for glioma. Hum Gene Ther. 2012 Oct;23(10):1043-53.

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Cat. No. M00097
Size Price
1 mg $98.00