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Catalog Products » Protein Analysis & Purification » Protein Detection and Immunoassay » Reagents for Protein Analysis » HRP-Protein L

HRP-Protein L

Description Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
Storage The product should be stored lyophilized until use. It remains stable in lyophilized form for at least one year if stored at 2-8°C. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to one year at -20°C or below. Avoid repeated freezing and thawing cycles.

The ideal working concentrations for each specific application should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points:
Dot blot: 1:2,000-1:20,000
Western blot: 1:2,000-1:20,000
ELISA: 1:20,000-1:200,000
Other applications: user-optimized

Cat. No. Product Name Quantity Price
HRP-Protein A - 1 mg
HRP-Protein G - 1 mg
Streptavidin-HRP - 1 mg
Biotin-Protein A - 2 mg
Biotin-Protein L - 1 mg

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Cat. No. M00098
Size Price
1 mg $95.00