Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.
CHO-K1/CCKB Stable Cell Line
M00154 | |
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$8,500.00 | |
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