Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB2 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB2 receptor agonist, neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 2). The EC50 of Neuromedin B on BB2 in CHO-K1 cells was 77.68 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/BB2 Stable Cell Line
M00182 | |
|
|
$8,500.00 | |
|
|
|
|
Ask us a question |