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Human Recombinant Glucagon-like Peptide 2 Receptor Stable Cell Line
CHO-K1/GLP2R/Gα15 Stable Cell Line
Figure 1. GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC50 of GLP-2 (1-34) on this cell was 0.11 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
The glucagon-like peptides include glucagon, GLP-1, and GLP-2 exert diverse actions on nutrient intake, gastrointestinal motility, islet hormone secretion, cell proliferation and apoptosis, as well as nutrient absorptionand assimilation. Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R). |
GLP-2 receptor, GLP-2-R, GLP-2R, glucagon-like peptide 2 receptor, glucagon-like peptide-2 receptor precursor, GLP-2, 9530092J08Rik
| Applications | Functional assay for GLP2 receptor |
| Storage | Liquid nitrogen immediately upon delivery |
| Species | Human |
| Freeze Medium | 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma) |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen) |
Figure 1. GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC50 of GLP-2 (1-34) on this cell was 0.11 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
RP12738-0.5 |
Glucagon-Like Peptide (GLP) I (7-37) - 0.5 mg |
$119.00 |
|
RP10773-0.5 |
Glucagon-Like Peptide (GLP) I (7-36), amide, human - 0.5 mg |
$90.00 |
|
RP10772-0.5 |
Glucagon (1-29), Human - 0.5 mg |
$90.00 |
|
RP10770-5 |
Glucagon (19-29), human - 5 mg |
$95.00 |
|
RP10770-1 |
Glucagon (19-29), human - 1 mg |
$24.00 |
|
RP10769-0.5 |
Glucagon-Like Peptide (GLP) II, human - 0.5 mg |
$90.00 |
For more documents, please visit "Technical Support".
| Cat. No. M00307 | ||
|---|---|---|
| Size | Price | |
| 2 vials | $8500.00 | |
