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Catalog Products » Other Products » Stable Cell Lines » Human Recombinant Glucagon-like Peptide 2 Receptor Stable Cell Line

CHO-K1/GLP2R/Gα15 Stable Cell Line

CHO-K1/GLP2R/Gα15 Stable Cell Line

Figure 1. GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC50 of GLP-2 (1-34) on this cell was 0.11 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

The glucagon-like peptides include glucagon, GLP-1, and GLP-2 exert diverse actions on nutrient intake, gastrointestinal motility, islet hormone secretion, cell proliferation and apoptosis, as well as nutrient absorptionand assimilation. Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R).

GLP-2 receptor, GLP-2-R, GLP-2R, glucagon-like peptide 2 receptor, glucagon-like peptide-2 receptor precursor, GLP-2, 9530092J08Rik

Applications Functional assay for GLP2 receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

  • CHO-K1/GLP2R/Gα15 Stable Cell Line
  • CHO-K1/GLP2R/Gα15 Stable Cell Line

    Figure 1. GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC50 of GLP-2 (1-34) on this cell was 0.11 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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RP10770-5
Glucagon (19-29), human - 5 mg
$95.00
RP10770-1
Glucagon (19-29), human - 1 mg
$24.00
RP10769-0.5
Glucagon-Like Peptide (GLP) II, human - 0.5 mg
$90.00
 


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Cat. No. M00307
Size Price
2 vials $8500.00