Catalog Products » Stable Cell Lines » Human Recombinant Mas-related G Protein Coupled Receptor Member X2 Stable Cell Line
CHO-K1/MRGPRX2 Stable Cell Line

Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Cortistatin 14. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Cortistatin 14 (Mean ± SD, n = 2). The EC50 of Cortistatin 14 on this cell was 0.64 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/MRGPRX2 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress mas-related G-protein coupled receptor member X2 (MRGPRX2). MRGPRX2 couples to both Gi and Gq alpha subunits in mast cells naturally, so that the PLC signaling can be triggered within the cells by agonist binding. This cell line is recommended for agonist screening and functional validations with calcium flux assay.
M00425
$8,500.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress mas-related G-protein coupled receptor member X2 (MRGPRX2). MRGPRX2 couples to both Gi and Gq alpha subunits in mast cells naturally, so that the PLC signaling can be triggered within the cells by agonist binding. This cell line is recommended for agonist screening and functional validations with calcium flux assay.
Applications Agonist screening and functional validations of MRGPRX2. 

Product Introduction
Expressed Gene Codon Optimized from NM_054030; no expressed tags
Target Protein NP_473371
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1X106 per vial in 1 ml) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 45% culture medium, 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

Background
Gene Synonyms MRGPRX2; MRGX2
Background MRGPRX2 (also known as MRGX2) is a Gq and Gi-coupled GPCR that binds to several peptides, including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminal peptide (PAMP). It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Dorsal root ganglia and adrenal chromaffin cells express MRGPRX2, and binding of these ligands to MRGPRX2 is thought to play a role in nociception and catecholamine release from the adrenal glands.

Examples
  • CHO-K1/MRGPRX2 Stable Cell Line
  • CHO-K1/MRGPRX2 Stable Cell Line

    Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Cortistatin 14. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Cortistatin 14 (Mean ± SD, n = 2). The EC50 of Cortistatin 14 on this cell was 0.64 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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