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HEK293/PD-L1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface expression of PD-L1 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.
$8500.00
M00544

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Product Introduction
Product Description Recombinant HEK293 cells stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface expression of PD-L1 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.
Culture Properties Adherent
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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Culture Conditions
Culture Medium DMEM, 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10566-016, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
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HEK293/PD-L1 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human PD-L1 on HEK293/PD-L1 cells. The HEK293/PD-L1 cells (blue) and the negative control HEK293 cells (red) were probed using Anti-Human PD-L1 (GenScript) and Alexa FluorTM 488-conjugated goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen). »

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.