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FACS analysis of PD-1 expression in Daudi/PD-1 cells.
| M00630 | |
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| $8,500.00 | |
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| Ask us a question | |
| Product Description |
Recombinant Daudi cells stably overexpress human programmed cell death
protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by
FACS analysis. |
| Applications | Binding assay or used as immunogen. |
| Expressed Gene | Codon optimized from NM_005018.2; no expressed tags |
| Target Protein | NP_005009 |
| Host Cell | Daudi |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 15 passages with no significant changes in assay
performance or expression profile. |
| Culture Properties | Suspension |
| Freeze Medium | 90% FBS (Cat. No. 10099-141, Gibco), 5% RPMI 1640 medium (Cat. No. 22400, Gibco), 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | RPMI 1640 medium, 20% FBS |
| Culture Medium |
RPMI 1640 Medium, 20% FBS, 0.5 μg/ml Puromycin (Cat. No. A11138-03,
Gibco), 1% Sodium Pyruvate (Cat. No. 11360-070, Gibco) |
| Gene Synonyms | PDCD1, CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 |
| Background |
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation
279), is a protein that in humans is encoded by the PDCD1 gene. PD-1,
functioning as an immune checkpoint, plays an important role in down regulating
the immune system by preventing the activation of T-cells, which in turn
reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1
is accomplished through a dual mechanism of promoting apoptosis (programmed
cell death) in antigen specific T-cells in lymph nodes while simultaneously
reducing apoptosis in regulatory T cells (suppressor T cells). |
FACS analysis of PD-1 expression in Daudi/PD-1 cells.
