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CHO-K1/HVEM Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human HVEM on CHO-K1/HVEM cells. The CHO-K1/HVEM cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-HVEM antibody (Cat. No. 318806, Biolegend).

CHO-K1/HVEM Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Homo sapiens TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against human HVEM or to measure binding affinity between HVEM and anti-HVEM antibodies.
M00646
$8500.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress Homo sapiens TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against human HVEM or to measure binding affinity between HVEM and anti-HVEM antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/HVEM Stable Cell Line
    • CHO-K1/HVEM Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human HVEM on CHO-K1/HVEM cells. The CHO-K1/HVEM cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-HVEM antibody (Cat. No. 318806, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.