|Synonyms||HRP Conjugated 25-Hydroxycholecalciferol, Calcidiol,Calcifediol|
|Description||25-Hydroxyvitamin D3 (25-OH vitamin D3) is measured worldwide to determine people’s vitamin D status by physicians. Its blood concentration is considered as the best indicator of vitamin D status in the body. Vitamin D is responsible for enhancing intestinal absorption of calcium. In humans, vitamin D3 and vitamin D2 are the most important compounds in the Vitamin D family. The deficiency of vitamin D can increase the risk of many diseases including autoimmune diseases, cancers and type II diabetes. 25-OH Vitamin D3 is formed in the liver by hydroxylation of vitamin D3 by the enzyme. It is converted in the kidneys into 1, 25-dihydroxyvitamin D3. 25-OH Vitamin D3 is also known as calcifediol, 25-Hydroxyvitamin D3, 25-Hydroxycholecalciferol.|
|Concentration||Phosphate buffered saline, pH 7.4, containing 50% glycerol 1% BSA and 0.01% thimerosal.|
|Storage||The conjugate can be stored for up to two years at -20°C or below.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by primary antibody affinity, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antigen for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.25-0.5 µg/ml
Other applications: user-optimized
For more documents, please visit "Technical Support".