MuLV Titer p30 ELISA Kit

Stable, accurate and reproducible MuLV titer determination

Features Product List Detection Principle Verification Data FAQ

Catalog Products » ELISA Detection Kits » MuLV Titer p30 ELISA Kit

Overview

The Murine Leukemia Virus (MuLV) is a retrovirus originally discovered in mice and has since been extensively used in gene delivery and transgenic research. MuLV vectors are commonly employed to transduce therapeutic genes into hematopoietic stem cells and T cells, aiming to correct genetic defects or enhance immune function. In the field of CAR-T therapy, MuLV vectors are particularly valuable for introducing CAR genes into T cells, thereby boosting their tumor recognition and cytotoxic capabilities. Additionally, MuLV holds significant promise in other cell therapies, such as NK cell engineering.

GenScript MuLV Titer p30 ELISA Kit (GenScript, L01041) is a sandwich ELISA kit designed to accurately and precisely measure the physical titer of MuLV by detecting the core protein p30 during viral vector production. This kit offers advantages such as stability, precision, accuracy, and efficiency, making it a cost-effective solution for minimizing rework and repeat testing. By improving experimental efficiency and increasing the qualification rate of production batches, the GenScript MuLV Titer p30 ELISA Kit is the preferred choice for MuLV detection, quality control, and batch release.

ELISA Kits

Features

Superior Stability

CV<10% for the same sample over four weeks, ideal for consistent long-term QC

High Accuracy

Recovery rate controlled at 80%-120%

Efficient Detection

Complete results in approximately 2 hours

Convenient Storage

All kit components are conveniently stored at 2–8°C

Product List

Cat. No.	Product Name	Quantity	Price	Order
L01041	MuLV Titer p30 ELISA Kit		$1000.00 96 Tests

Detection principle

Schematic diagram

The GenScript MuLV Titer p30 ELISA Kit employs a double-antibody sandwich ELISA method for highly specific and accurate detection of MuLV.

During the assay, standards or samples are added to a capture plate pre-coated with an anti-MuLV p30 monoclonal antibody, allowing the MuLV in the sample to be selectively captured. A biotin-labeled anti-MuLV p30 monoclonal antibody is then introduced as the detection antibody, forming a complex with the captured MuLV. This is followed by the addition of streptavidin-HRP, which binds to the biotin-labeled detection antibody, amplifying the signal.

After thorough washing, a TMB substrate solution is added to trigger a colorimetric reaction. The reaction is stopped by adding a stop solution, which changes the color from blue to yellow. The absorbance is measured at 450 nm and 650 nm using a microplate reader. The MuLV concentration in the sample is then quantified with high precision based on a standard curve.

Figure 1. Schematic diagram of detection principle

Performance Advantage in Market Comparison

Comparison on Stability

Figure 2. Comparison of stability of MuLV Titer p30 ELISA kit over four weeks
In a four-week study, the GenScript MuLV Titer p30 ELISA Kit and a competitor's product were used to test the same sample. GenScript's kit demonstrated superior stability, maintaining highly consistent detection values throughout the study period.
Comparison on Batch Consistency

In batch-to-batch precision analysis, GenScript's MuLV Titer Detection Kit demonstrated highly consistent standard curves, simplifying data analysis across different batches compared to competitors.

Figure 3. Comparison of standard curves across different batches of GenScript kits

Figure 4. Comparison of standard curves across different batches of competitor kits

Comparison on Sample Dilution Linearity

In the dilution linearity analysis, GenScript's MuLV Titer Detection Kit and a competitor's kit were used to test the same sample diluted by N-fold and 4N-fold. The results showed that GenScript's kit provided accurate and reliable test results across different dilution factors.

Table 1. Comparison of sample dilution linearity

Sample No.	CV of Sample at Different Dilution
Sample No.	GenScript	Competitor C
Sample 1	4.8%	29.4%
Sample 2	0.4%	17.9%
Sample 3	5.9%	22.7%
Sample 4	7.8%	5.6%
Sample 5	12.9%	51.9%
Sample 6	11.3%	6.2%
Sample 7	3.4%	45.6%
Sample 8	5.1%	15.6%
Sample 9	9.6%	38.3%
Sample 10	0.5%	22.0%

Verification data

Dilution Linearity

Figure 5. Dilution linearity assessment of L01041
The dilution linearity of this kit was analyzed by diluting samples of different unknown concentrations at different ratios. The results showed that when using the GenScript kit to calculate the concentration of the same sample at different dilution factors, the CV was consistently below 15%, indicating minimal influence from dilution factors and sample matrix.

Precision Analysis

Figure 6. Intra-batch and inter-batch precision analysis of L01041
Samples of different concentrations were tested using different batches of the kit, with each sample being tested 10 times. The results showed that the intra-batch CV was highest at 4.61%, and the inter-batch CV was highest at 4.17%, indicating good intra- and inter-batch consistency of the kit's detection results.

Table 2. Intra- and inter-assay precision of the MuLV Titer p30 ELISA kit

Theoretical Conc. (ng/mL)	Intra-assay (n=10)			Inter-assay (n=30)
Theoretical Conc. (ng/mL)	Average Measured Conc. (ng/mL)	CV	Accuracy	Average Measured Conc. (ng/mL)	CV	Accuracy
4	3.91	3%	98%	3.89	4%	97%
2	1.91	3%	96%	1.85	4%	93%
0.5	0.44	1%	88%	0.43	4%	86%

Recovery rate

Figure 7. Recovery rate of L01041 in different samples
Different concentrations of MuLV p30 standard were added to samples of different unknown concentrations. The recovery of MuLV p30 spiked in each sample was stable between 80% and 120%.

FAQ

1. How is the physical titer (VP/mL) of MuLVs calculated?

Calculated according to the following two formulas:
p30 sample concentration (ng/mL) = p30 test concentration (ng/mL) × dilution factor
MuLV titer (VP/mL) = p30 sample concentration (ng/mL) × 9.009 × 10⁶
This formula is based on the fact that each MuLV viral particle (VP) contains approximately 2187 p30 molecules.
1 VP contains 2187 × 30.61 × 10³ / (6 × 10²³ ) g p30 ≈ 1.11 × 10⁻⁷ ng p30 or 1 ng p30 ≈ 9.009 × 10⁶ VP.
2. How many samples can be tested with one kit?

The MuLV Titer p30 ELISA Kit (GenScript, L01041) includes a pre-coated 96-well microplate with 8 strips of 12 wells each. We recommend preparing 7 standard working solutions according to the dilution gradient for the standard curve outlined in the instructions. To ensure reliable titer quantification, it is advised to test both the standards and samples in duplicate. This allows for the testing of up to 41 samples with one kit.
3. When using a plate washer for an assay that requires more wash buffer, are there additional wash buffer reagents available?

Yes, 20× Wash Solution (GenScript, B00063) can be purchased separately and is suitable for use with this kit.