Overview

Single-stranded DNA (ssDNA or ssODN) is proven to be the best CRISPR homology directed repair (HDR) template for creating gene knock-in, with high editing efficiency and reduced off-target integration. GenScript now offers high quality, sequence verified ssDNA for maximizing the editing efficiency of your CRISPR experiments.

Why Use ssDNA as CRISPR Gene Knock-In HDR Templates?

  • High editing efficiency
  • Lower cytotoxicity
  • Reduced off-target integration
  • Increased editing accuracy
  • Ideal for editing primary cells and stem cells
  • Ideal for developing transgenic animal model

Why GenExact™ ssDNA?

  • Sequence verification by sanger sequencing the final ssDNA product
  • No harsh chemicals, enzymatic approach for ensuring non-detectable levels of dsDNA and minimum DNA base damage
  • Up to 100 µg delivery quantity allowing for flexible study design
  • Free life-time gene template storage supporting faster and more cost-effective re-orders
  • Expertise & 16+ year experience in synthesizing difficult genes as ssDNA templates

Testimonials

KansasStateDr. Theodore Roth

the Marson Lab at University of California San Francisco

Long ssDNA sequences are difficult to produce in the lab, especially at the high concentrations necessary for gene editing experiments, we were able to successfully integrate large DNA sequences into primary human T cells using GenScript's long ssDNA product.

Services

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ssDNA

Deliver in as fast as 3 weeks

Quantity (ug) Research Grade (up to 2mg) Preclinical Grade (starting from 50ug)
151-500nt ($/Item) 501-4000nt ($/nt) 151-500nt ($/Item) 501-4000nt ($/nt)
3 $400 $0.80 - -
5 $550 $1.00 - -
10 $800 $1.30 - -
20 $1,300 $1.90 - -
50 $1,850 $3.00 $2,405 $3.54
100 $2,400 $4.40 $3,120 $5.00
>100 Email Us

* We add-on extra Bioburden, endotoxin and protein residue test for preclinical grade ssDNA synthesis.

Test Specifications Detection Method Release Criteria Research Grade (≤2mg) Preclinical Grade (≥50ug)
Purity Agarose gel electrophoresis Single band
Sequence accuracy Sanger sequencing 100% sequence alignment
Optical density Spectrophotometer at 260 nm/230 nm ≥ 2.0
Spectrophotometer at 260 nm/280 nm 1.8~2.0
Bioburden Incubation in TSA plate No colony formation -
Endotoxin Qualitative TAL assay < 10 EU/mg -
Protein Residue Micro BCA Protein Assay Kit ≤50ug/mg -
Quantitative purity Agilent 2100 Bioanalyzer ≥90% - Add-on
pH pH meter with pH probe Depending on buffer pH - Add-on
Conductivity pH meter with conductivity probe Depending on buffer conductivity - Add-on

Resources

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Customer Reviews

17 Customer Reviews

4.5 out of 5 stars

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