Overview

6xHistidine and Glutathione S-transferase (GST) are commonly used protein/peptide tags added to the end of a recombinant protein to aid in their purification or detection. GenScript Ni-Charged MagBeads and Glutathione MagBeads provide simple, rapid and reliable methods for micro-scale purification of His- and GST-tagged fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cell culture.

Ni-charged or Glutathione MagBeads Workflow

The Ni-Charged MagBeads are pre-charged with nickel and ready to use for micro-scale purification of 6xHis-tagged proteins under native or denaturing conditions. The Glutathione MagBeads are immobilized with Glutathione mainly through its central carbon on the beads surface for efficient GST binding. Read More »

Experimental Data and Citations Database

Lane 1, Bacterial lysate expressing GST Tag.
Lane 2, Purified GST Tag (26 kDa) using Glutathione MagBeads.
Lane 3, Bacterial lysate expressing His-tagged protein.
Lane 4, Purified His-tagged protein (27 kDa) using Ni-charged MagBeads.

Fig.2. Purification of GST Tag and His-tagged Protein using GenScript Micro-scale Tagged Protein Purification MagBeads. Lane M, Protein Molecular Weight Standard.

Ordering Information

Cat. No. Product Name Quantity Price Order
L00295
Ni-charged MagBeads
$98.00 8 ml (2 ml Settled Beads)
 


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