Oligo Quality Control Service

Divide the sample into many small reaction units and perform PCR amplification in each unit to quantify the copy number of target accurately.

Advantages:

• Absolute quantification, no need to plot a standard curve, more efficient
• High sensitivity, capable of detecting low copy number target sequences.

• Quantification of plasmid copy number for target sequence or impurity
• Absolute quantification of standard products
• Low frequency mutation detection
• CRISPR KI&KO efficiency detection
• More precise detection for E.Coli genomic DNA residue

The sample must be electrophoresed on a polyacrylamide gel, resulting in different bands according to molecular weight. By comparing the sample bands to a Marker, the purity of the target component is determined.

Advantages:

• Cost-effective, semi-quantitative, simple, and fast
• Expedited purity reports, available within 1 day
• Avoid sample cross-contamination effectively

Identify purity of complex sequences, such as annealed double-stranded oligo, degenerate oligo, long sequences, etc. Suitable for the screening stage of candidate molecules.

• DNA Oligo: 20-130 nt such as NGS adapters etc.
• RNA Oligo: 20-130 nt such as sgRNA, siRNA, etc.

Based on capillary electrophoresis (CE) technology, the purity of the sample is quantified by comparing the peak area of the sample to the area of a known concentration of marker or ladder.

Advantages:

• Enhance data precision and repeatability, shorten analysis time
• Minimize sample consumption

Detect impurity such as N-1, metal chelation, desulfurization, depurination etc., utilizing the capability of liquid chromatography to separate compounds as well as mass spectrometry to identify components. Determining purity, and carry out component identification.

Advantages:

• Identify molecular weight of target sequence, mass accuracy to 500 ppm
• Identify purity of target sequence, provide information on impurity molecular weight and proportion

Semi-quantitative detection of endotoxins by Limulus Amebocyte Lysate (LAL) reagent to avoid endotoxin interference on downstream experiments. Provides options for <1 EU/mg, <2 EU/mg, or other customized request.

Advantages:

• Customized optimization processes to control endotoxin levels strictly
• Customized detection standards to meet different requirements of endotoxin levels

The mass detection alone cannot fully confirm the accuracy of the synthesized sequence. LC MSMS sequencing, on the other hand, can ensure the accuracy of the sequence arrangement and different modifications on the sequence. LC MSMS sequencing is implemented by matching the product ion masses (m/z at MS2 level) with the theoretical MS/MS spectrum.

Advantages:

• Support identification of both full sequence and modifications
• Short detection time, as fast as 1 hour
• High mass accuracy of 10 ppm

• RNA within 200nt and DNA within 100nt can achieve 100% sequence coverage, such as sgRNA, siRNA, ASO, etc.
• RNA above 200nt can achieve over 80% sequence coverage, such as mRNA.

Utilize sequence by synthesis (SBS), where fluorescence signals are recorded by optical equipment and converted into base information through computer analysis.

Sequencing of single-stranded and double-stranded DNA, RNA samples, or library samples, such as:

• NGS adapter cross-contamination rates
• NGS probe coverage and uniformity
• Oligo pool coverage and uniformity

Nanopore platform: Nanopore sequencing technology based on electrical signal detection.

Advantages:

• Do not need fragmentation/joining, allowing for direct sequencing for long sequences

Sequencing of single-stranded and double-stranded DNA, RNA samples, or library samples, such as:

• Detect integration sites in CRISPR-Cas9 mediated gene editing, particularly suitable for the precision testing of long insertions or long insertions with repetitive sequences
• Sequencing of long-chain ssDNA

Test for the presence of human genomic DNA template contamination in samples, and also support the customized detection of exogenous contaminants such as E.coli.

Advantages:

• High sensitivity, consistent detection results, rapid testing
• Support both single- and multi-plex detection systems

For orders requiring control of exogenous genes and environmental contamination. Identify the type and degree of exogenous contamination through specific TaqMan probes.

• Quality control of raw materials for molecular diagnostic such as NGS/qPCR oligos

Test to find whether qPCR probes and primers exhibit abnormal amplification signals in the negative control group without positive control templates and sample templates, thereby avoiding false positives.

Advantages:

• High sensitivity, consistent detection results, rapid testing
• Support both single- and multi-plex detection systems

By detecting the increase in fluorescence post-enzyme digestion, confirm the fluorescence functionality of the dual-labeled oligos. Make sure the fluorescence and quenching functionality of dual-labeled oligos such as TaqMan probes and molecular beacons, allowing for rapid testing.

Advantages:

• Dnase I enzyme is used for regular sample
• Self-developed Benz-Neburase™ high-efficiency enzyme is used for sequences with low DNase I digestion efficiency, special sequences and special fluorescent labels

• TaqMan probe applied in molecular diagnostic
• Molecular beacon in the field of gene expression