GenCRISPR Knock-Out Cell Lines or Cell Pools
(required fields)
1 Service Information
2 Verification
3 Submission

Please answer all required questions (marked with *) and fill in as many optional questions as possible. Upon submission, you will receive an email confirmation of your inquiry. Assuming all required information has been provided, our service representative will get back to you with a free quote within 1 business day.

Or you can also download the offline version of the request form, fill it out, and email it back to us.

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Gene Information

Single gene knockout in a cell line
Multiplex gene knockout in a cell line.
* Name of Target Gene:
* NCBI Accession Number (Gene ID) (put “unknown” if unknown):
No
Yes, I need to target all transcriptional isoforms.
Yes, I need to target specified gene product.
Wide-type SpCas9    Enhanced specificity SpCas9 (eSpCas9)

Enhanced specificity SpCas9 (eSpCas9) is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016). Cas9 genome editing is dependent on the separation of DNA double strands, and mismatches between sgRNA and untargeted DNA sequences can cause unspecific binding and cleavage. To improve genome editing specificity, eSpCas9 or SpCas9 with mutations K848A, K1003A, and R1060A was developed. Neutralization of these positively charged residues within the non-target strand groove of SpCas9 weakened non-target binding and encouraged on-target binding which requires more stringent Watson-Crick base pairing. eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency.

Yes    No    Not sure
Yes    No    Not sure

Host Cell Line Information

* Name of Target Host Cell Line:
Alternative Cell Line:
Yes
No. But I can provide a biosafety certificate for the cell line.
No. I can not provide any certificate for the cell line. The cell line may not be taken and an alternative cell line is suggested.
Client (Note: Cell line must be mycoplasma-free. Suggested mycoplasma detection kit: MycoAlert™ PLUS Mycoplasma Detection Kit-Lonza, LT07-701)
GenScript (Note: Only for ATCC cell lines and extra fee and time may be required)
Adherent   Suspension    Both   
Medium:
Additives:
The cells are detached with (e.g. trypsin) for minutes.
The cell line is passaged: times per week in a ratio:
Yes    No, the cell line may not be taken and an alternative cell line is suggested   Not sure
Yes    No    Not sure
Yes No   

Deliverables and QC Methods

I just need CRISPR knockout cell pools    I need CRISPR knockout cell lines
Single allele knockout    Bi-allelic knockout    All-alleles knockout (Allele number is unknown)
One clone (default)    Two clones    Three clones    More clones
6 months for free    12 months    18 months    More
Immediately    Within one month    Within three months    Half a year later   

Additional Information (Optional)

One copy    Two copies    More    Not sure   
Please cite evidence to support your conclusion:
Haploid    Diploid    More    Not sure   
Please cite evidence to support your conclusion:
Antibiotic selection (Blasticidin and/or puromycin)    FACS sorting
Chemical transfection, please specify the reagent:
Electroporation, please specify the program:
Nucleofection, please specify the kit and program:
Lentivirus
Provide transfection efficiency for the suggested method if possible:
CMV    CBh    EF1-a    Not sure   
If it is not listed above, please specify:
Yes, see below    No   
Please provide the protocol with information about the cell line and any special growth characteristics or requirements:
Gene function analysis. Comment:
Assay development. Comment:
Drug screening. Comment:
In vivo tumorigenesis. Comment:
Manufacturing host. Comment:
Other. Please indicate your specific application and requirements: