Catalog Products » Protein Isolation and Analysis » Protein Electrophoresis » Precast Gels

20% off any SurePAGE order, use promo code SurePAGE20%OFF,
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GenScript's Precast Gels High resolution & Robust Separation

Large well volume – Up to 80 µl for diluted samples
High resolution – Even, sharp bands, guaranteed lot-lot consistency
Long shelf life – Up to 12 months at 2-8℃
Cost effective – 30-50% price reduction compared to other major competitors
Compatible cassette design – Fits all popular mini-gel tanks

GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.

GenScript's gel series include-SurePAGE™ Gels which are the premium, higher resolution and reproducibility gels, and ExpressPlus, good quality and very cost-effective gels.

View the detailed comparison between SurePAGE,
ExpressPlus and other major gel types.

ExpressPlus (Bis-Tris)
SurePAGE (Bis-Tris)
Competitor B (Tris-Glycine)
Homemade (Tris-Glycine)
Competitor T (Bis-Tris)

SurePAGE™ Bis-Tris Gels (10x8)

Free samples of SurePAGE™ (Including MOPS buffer powder) available. Contact us freesample@genscript.com.

SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.

Superior resolution

SurePAGE
(Bis-Tris)
Competitor B
(Tris-Glycine)
Homemade
(Tris-Glycine)
Competitor T
(Bis-Tris)

Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.

Cost effective
Easier loading, Larger well volumes

GenScript Precast gel selection guide

Step 1. Check the compatibility of your gel tank

Compatible gel tanks

Bio-Rad Mini-PROTEAN^® II & 3*

Bio-Rad Mini-PROTEAN^®Tetra System*

Invitrogen Novex XCell I, II, & Surelock^® (Use with GenScript Gel Tank Adapter Plates)

LONZA PAGEr^® Minigel Chamber

Hoefer Mighty Small (SE 260/SE 250)

Hoefer Tall Mighty Small (SE 280)

*please reverse the gasket, see instructions below

Step 2. Choose the gel that best separates your protein of interest based on the migration chart below

With MOPS running buffer

With MES running buffer

Step 3. Choose the appropriate well number based on the number of samples and sample volume

Gel series	Well No.	Recommended Max. loading V./well	Well size	Recommended amount of protein/well
10x8 Gels	10	60µl	80µl	60µg
	12	45µl	60µl	50µg
	15	30µl	40µl	40µg

Step 4. Choose LDS based sample loading buffer

For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.

BUY MORE SAVE MORE
Free MOPS running buffer always included! Buy 10 boxes or more and save $10/box!
Buy 5 units (20x5 gels) or more and save $20/unit!

Resources
Gel Selection Tool ExpressPlus (10x8) manual SurePAGE (10x8) manual ExpressPlus (10x8) quick guide SurePAGE (10x8) quick guide

SurePAGE™ ordering

SurePAGE™ 10x8 gels ordering list

Cat. No.	Product Name	Price
M00652	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-12%, 10 wells - 10 PK/BOX	$85.00
M00653	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-12%, 12 wells - 10 PK/BOX	$85.00
M00654	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-12%, 15 wells - 10 PK/BOX	$85.00
M00655	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-20%, 10 wells - 10 PK/BOX	$85.00
M00656	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-20%, 12 wells - 10 PK/BOX	$85.00
M00657	SurePAGEᵀᴹ, Bis-Tris, 10x8, 4-20%, 15 wells - 10 PK/BOX	$85.00
M00658	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8-16%, 10 wells - 10 PK/BOX	$85.00
M00659	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8-16%, 12 wells - 10 PK/BOX	$85.00
M00660	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8-16%, 15 wells - 10 PK/BOX	$85.00
M00661	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8%, 10 wells - 10 PK/BOX	$85.00
M00662	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8%, 12 wells - 10 PK/BOX	$85.00
M00663	SurePAGEᵀᴹ, Bis-Tris, 10x8, 8%, 15 wells - 10 PK/BOX	$85.00
M00664	SurePAGEᵀᴹ, Bis-Tris, 10x8, 10%, 10 wells - 10 PK/BOX	$85.00
M00665	SurePAGEᵀᴹ, Bis-Tris, 10x8, 10%, 12 wells - 10 PK/BOX	$85.00
M00666	SurePAGEᵀᴹ, Bis-Tris, 10x8, 10%, 15 wells - 10 PK/BOX	$85.00
M00667	SurePAGEᵀᴹ, Bis-Tris, 10x8, 12%, 10 wells - 10 PK/BOX	$85.00
M00668	SurePAGEᵀᴹ, Bis-Tris, 10x8, 12%, 12 wells - 10 PK/BOX	$85.00
M00669	SurePAGEᵀᴹ, Bis-Tris, 10x8, 12%, 15 wells - 10 PK/BOX	$85.00

Order 10 boxes or more and save $10/box!

ExpressPlus™ ordering

ExpressPlus™ 10x8 gel ordering list

Cat. No.	Product Name	Price
M42010	ExpressPlus™ PAGE Gel,10×8, 4-20%, 10 wells - 20 Gels	$140.00
M42012	ExpressPlus™ PAGE Gel,10×8, 4-20%, 12 wells - 20 Gels	$140.00
M42015	ExpressPlus™ PAGE Gel,10×8, 4-20%, 15 wells - 20 Gels	$140.00
M41210	ExpressPlus™ PAGE Gel,10×8, 4-12%, 10 wells - 20 Gels	$140.00
M41212	ExpressPlus™ PAGE Gel,10×8, 4-12%, 12 wells - 20 Gels	$140.00
M41215	ExpressPlus™ PAGE Gel,10x8, 4-12%, 15 wells - 20 Gels	$140.00
M81610	ExpressPlus™ PAGE Gel,10×8, 8-16%, 10 wells - 20 Gels	$140.00
M81612	ExpressPlus™ PAGE Gel,10×8, 8-16%, 12 wells - 20 Gels	$140.00
M81615	ExpressPlus™ PAGE Gel,10×8, 8-16%, 15 wells - 20 Gels	$140.00
M01010	ExpressPlus™ PAGE Gel,10×8, 10%, 10 wells - 20 Gels	$140.00
M01012	ExpressPlus™ PAGE Gel,10×8, 10%, 12 wells - 20 Gels	$140.00
M01115	ExpressPlus™ PAGE Gel,10×8, 10%, 15 wells - 20 Gels	$140.00
M00810	ExpressPlus™ PAGE Gel,10×8, 8%, 10 wells - 20 Gels	$140.00
M00812	ExpressPlus™ PAGE Gel,10×8, 8%, 12 wells - 20 Gels	$140.00
M00815	ExpressPlus™ PAGE Gel,10×8, 8%, 15 wells - 20 Gels	$140.00
M01210	ExpressPlus™ PAGE Gel,10×8, 12%, 10 wells - 20 Gels	$140.00
M01212	ExpressPlus™ PAGE Gel,10×8, 12%, 12 wells - 20 Gels	$140.00
M01215	ExpressPlus™ PAGE Gel,10×8, 12%, 15 wells - 20 Gels	$140.00

Related products

Cat. No.	Product Name	Price
L00671	Tank Adaptor - 2 pcs/unit	$12.00
L00674	Cassette Opener - 1 kit	$10.00
L00699	Buffer Dam - 1 pc	$8.00
M00676-10	4X LDS Sample Buffer - 10 ml	$10.00
M00676-250	4X LDS Sample Buffer - 250 ml	$90.00
M00677	MES SDS Running Buffer Powder - 1 box(5pcs)	$25.00
MB01015	5X Sample Buffer - 5 ml	$24.00
M00138	Tris-MOPS-SDS Running Buffer Powder - 1 Box (5/PK)	$25.00
M00139	Transfer Buffer Powder - 1 Box (10/PK)	$58.00
M00624-250	Broad Multi Color Pre-Stained Protein Standard - 250 µl	$70.00
M00624-1250	Broad Multi Color Pre-Stained Protein Standard - 1250 µl (250µl x 5)	$280.00

*Tank Adaptor(Cat. No. L00671) is for use with Invitrogen Novex XCell I, II, & Surelock^®

FAQ

FAQ

What's the difference between ExpressPlus™ and SurePAGE™ gels?

SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency compared to ExpressPlus™ gels.
How long does it take to run a ExpressPlus™ or SurePAGE™ gel?

It depends on the concentration of the gel. Usually it takes about 50 minutes at 140V. Shorter running times can be achieved if a higher voltage is used, but higher than 180V is not recommended.
What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?

Any tank that is designed to run 10cm x 8cm (length x width) precast gels. See product page for the list.
Is this gel stable at room temperature?

Yes. SurePAGE™ and ExpressPlus™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.
Which sample loading buffer should I choose?

We recommend using 4xLDS sample buffer (M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.
What running buffer should I use to run the gel?

We recommend MOPS for large and medium proteins, and MES for small proteins. Tris-glycine buffer is NOT compatible with GenScript's precast gels.
What's the buffer I should use to transfer the gel?

We recommend Tris Bicine transfer buffer (M00139).
Why do some parts of the gel become sticky following protein transfer?

This is caused by the concentrated polyacrylamide on the top part of the gel. It will help if this part of the gel is cut and removed before transfer.
Will overnight destaining affect the resolution of the band?

It depends. We suggest using a destaining solution consisting of 15% methanol and 10% acetic acid in DI water for overnight destaining.

Trouble shooting

Problems	Probable cause	Solution
Distorted protein bands	Air bubbles in the sample wells	Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading
Some part of the tracking dye changed to yellow	Buffer enters gel because of broken cassette	Gel tank is not compatible or cassette was damaged
Some part of the tracking dye changed to yellow	pH value decreased	Prepare new running buffer with deionized water. Check pH
Streaking	Insoluble or weakly charged particles (such as carbohydrates) in sample	Heat sample in the presence of SDS, centrifuge sample and load the supernatant
Electrophoresis time is too long	Seal is not removed from the bottom of the cassette	Peel the seal off from the bottom of cassette before loading
Electrophoresis time is too long	Incorrect running conditions	Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis
Bands are not well separated	Incorrect gel percentage	Use the protein migration table to choose the appropriate gel
	Sample overloading	Reduce sample loading amount, especially when the sample contains many kinds of protein.
	Insufficient SDS in loading buffer	Increase SDS content in the sample during preparation
	Insufficient buffer to keep tank cool	Add more buffers to the outer tank until it's at the same level or above the top of the sample wells
Sample spreading across the gel	Sample contains too much salt	Reduce salt content by dialysis or ultra-filtration
The voltage cannot reach the set value	Leaking between the inner and outer tank during run	Use compatible gel tank
The voltage cannot reach the set value	Excess salt in the sample	Reduce salt content by dialysis or ultra-filtration
Lots of air bubbles between the gel and the cassette	Running buffer is hot after electrophoresis	Add more running buffer to the outer tank

Technical Resources

GenScript's Precast Gels High resolution & Robust Separation

ExpressPlus (Bis-Tris)

SurePAGE (Bis-Tris)

Competitor B (Tris-Glycine)

Homemade (Tris-Glycine)

Competitor T (Bis-Tris)

SurePAGE™ Bis-Tris Gels (10x8)

Superior resolution

SurePAGE

(Bis-Tris)

Competitor B

(Tris-Glycine)

Homemade

(Tris-Glycine)

Competitor T

(Bis-Tris)

Cost effective

Easier loading, Larger well volumes

GenScript Precast gel selection guide

Compatible gel tanks

Bio-Rad Mini-PROTEAN® II & 3*

Bio-Rad Mini-PROTEAN®Tetra System*

Invitrogen Novex XCell I, II, & Surelock® (Use with GenScript Gel Tank Adapter Plates)

LONZA PAGEr® Minigel Chamber

Hoefer Mighty Small (SE 260/SE 250)

Hoefer Tall Mighty Small (SE 280)

With MOPS running buffer

With MES running buffer

For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

SurePAGE™ 10x8 gels ordering list

ExpressPlus™ 10x8 gel ordering list

FAQ

What's the difference between ExpressPlus™ and SurePAGE™ gels?

How long does it take to run a ExpressPlus™ or SurePAGE™ gel?

What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?

Is this gel stable at room temperature?

Which sample loading buffer should I choose?

What running buffer should I use to run the gel?

What's the buffer I should use to transfer the gel?

Why do some parts of the gel become sticky following protein transfer?

Will overnight destaining affect the resolution of the band?

Trouble shooting

Bio-Rad Mini-PROTEAN^® II & 3*

Bio-Rad Mini-PROTEAN^®Tetra System*

Invitrogen Novex XCell I, II, & Surelock^® (Use with GenScript Gel Tank Adapter Plates)

LONZA PAGEr^® Minigel Chamber