High Resolution & Robust Separation
- Large well volume – Up to 80 µl of sample volume
- High resolution – Even, sharp bands, guaranteed lot-lot consistency
- Long shelf life – Up to 24 months at 2-8℃; Gels are also stable for 3 months at room temperature within 1 year after manufacturing date.
- Cost effective – Free running buffer; 30-50% price reduction compared to other major competitors
- Compatible cassette design – Compatible with Bio-Rad mini gel tank
GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.
GenScript's gel series include:
- SurePAGE™ Gels: premium, high resolution and reproducibility gels
- ExpressPlus™ Gels: good quality and very cost-effective gels
View the detailed comparison between SurePAGE,
ExpressPlus™ and other major gel types.
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ExpressPlus™ (Bis-Tris)
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SurePAGE™ (Bis-Tris)
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Competitor B (Tris-Glycine)
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Homemade (Tris-Glycine)
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Competitor T (Bis-Tris)
"Our area of interest is in Developmental Biology and we are interested in proteins that are involved in transcriptional regulation, autophagy, mitophagy, apoptosis. GenScript's SurePAGE™ precast gels provides an economical and quality option for our research needs and saves us at least an hour during gel electrophoresis."
―Pragnya Das, Drexel University
SurePAGE™ Bis-Tris Gels (10x8)
Free samples of SurePAGE™ (Including MOPS buffer powder) available. Contact us freesample@genscript.com.
SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.
Superior resolution
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SurePAGE
(Bis-Tris)
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Competitor B
(Tris-Glycine)
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Homemade
(Tris-Glycine)
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Competitor T
(Bis-Tris)
Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5 µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.
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Cost effective
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Easier loading, Larger well volumes
GenScript Precast Gel Selection Guide
GenScript's high quality mini gels generate the best electrophoresis performance with GenBox Mini Tank.
GenBox Mini Tank is designed for fast and consistent running of 2 mini gels at high voltages.
Other compatible gel tanks
Bio-Rad Mini-PROTEAN® II & 3*
Bio-Rad Mini-PROTEAN® Tetra System*
*Please reverse the gasket on the inner electrode assembly before use. See manual for detail.
With MOPS running buffer
With MES running buffer
| Gel series | Well No. | Recommended Max. loading V./well | Well size | Recommended amount of protein/well |
|---|---|---|---|---|
| 10x8 Gels | 10 | 60 µl | 80 µl | 60 µg |
| 12 | 45 µl | 60 µl | 50 µg | |
| 15 | 30 µl | 40 µl | 40 µg |
For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.
An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.
SurePAGE™ 10x8 gels ordering list
ExpressPlus™ 10x8 gel ordering list
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00671 |
Tank Adaptor - 2 pcs/unit |
$12.00 |
|
L00674 |
Cassette Opener - 1 kit |
$10.00 |
|
L00699 |
Buffer Dam - 1 pc |
$8.00 |
|
L00780 |
GenBox Mini Electrophoresis tank - 1 unit |
$480.00 |
|
L00781 |
GenBox Mini Blot module - 1 unit |
$500.00 |
|
M00676-10 |
4X LDS Sample Buffer - 10 ml |
$12.00 |
|
M00676-250 |
4X LDS Sample Buffer - 250 ml |
$100.00 |
|
M00677 |
MES SDS Running Buffer Powder - 1 box(5pcs) |
$25.00 |
|
MB01015 |
5X Sample Buffer - 5 ml |
$24.00 |
|
M00138 |
Tris-MOPS-SDS Running Buffer Powder - 1 Box (5/PK) |
$25.00 |
|
M00139 |
Transfer Buffer Powder - 1 Box (10/PK) |
$58.00 |
|
M00624-250 |
Broad Multi Color Pre-Stained Protein Standard - 250 µl |
$72.00 |
|
M00624-1250 |
Broad Multi Color Pre-Stained Protein Standard - 1250 µl (250µl x 5) |
$288.00 |
*Tank Adaptor(Cat. No. L00671) is for use with Invitrogen Novex XCell I, II, & Surelock®
Gel Specifications
| Gel Thickness | 1.0 mm |
| Gel Chemistry | Bis-Tris |
| Gel Format | Mini Gels (10 x 8 cm) |
| Storage | 2-8 ℃ |
| Shelf Life | 24 months |
| Running Buffer Recommendations | Tris-MOPS SDS (Cat. No. M00138) or MES SDS (Cat. No. M00677) |
FAQ
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What's the difference between ExpressPlus™ and SurePAGE™ gels?
SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency, and shorter running time compared to ExpressPlus™ gels.
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How long does it take to run an ExpressPlus™ or SurePAGE™ gel?
It depends on the gel type and concentration. SurePAGE™ gels can run at 200V for 20 minutes using MES running buffer or 30 minutes using MOPS running buffer. ExpressPlusTM gels run at 140V for 50 minutes.
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What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?
GenScript’s gels generate the best performance using GenBox Mini Gel tank and are also compatible with Bio-Rad Mini Gel Tanks (see manual for user instructions). Other gel tanks are not recommended.
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Is this gel stable at room temperature?
Yes. SurePAGE™ and ExpressPlus™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.
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Which sample loading buffer should I choose?
We recommend using 4xLDS sample buffer (Cat. No. M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.
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What running buffer should I use to run the gel?
We recommend MOPS for large and medium proteins (greater than 30kDa), and MES for small proteins (smaller than 30kDa). Tris-glycine buffer is NOT compatible with GenScript's precast gels.
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What's the buffer I should use to transfer the gel?
We recommend Tris Bicine transfer buffer (M00139).
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Will overnight destaining affect the resolution of the band?
For overnight destaining, we suggest using a weaker destaining solution consisting of 15% methanol and 10% acetic acid in water.
Trouble shooting
| Problems | Probable cause | Solution |
|---|---|---|
| Distorted protein bands | Air bubbles in the sample wells | Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading |
| Some part of the tracking dye changed to yellow | Buffer enters gel because of broken cassette | Gel tank is not compatible or cassette was damaged |
| Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage |
|
|
| Streaking | Insoluble or weakly charged particles (such as carbohydrates) exist in the sample | Heat up the sample in the presence of SDS, centrifuge sample and load the supernatant |
| Electrophoresis time is too long | Check the specifications of the power supply if it can support multiple gel runs at the same time | Peel the seal off from the bottom of cassette before loading |
| Incorrect running conditions | Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis | |
| Electrophoresis does not start with error shown on the power supply | Seal is not removed from the bottom of the cassette | Peel the seal off from the bottom of cassette before loading |
| Bands are not well separated | Incorrect gel percentage | Use the protein migration table to choose the appropriate gel |
| Sample overloading | Reduce sample loading amount, especially when the sample contains many kinds of protein. | |
| Insufficient SDS in loading buffer | Increase SDS content during sample preparation | |
| Insufficient buffer to keep tank cool | Add more buffers to the outer tank until it is at the same level or above the top of the sample wells | |
| Sample spreading across the gel | Sample contains too much salt | Reduce sample salt content by dialysis or ultra-filtration |
| The voltage cannot reach the set value | Leaking between the inner and outer tank during run | Use compatible gel tank |
| Run too many gels on the same power supply | Check the specifications of the power supply if it can support multiple gel runs at the same time | |
| Lots of air bubbles between the gel and the cassette | Overheat might be the cause, especially if running buffer is hot after electrophoresis | Add more running buffer to the outer tank |
| Sample do not separate or migrate with a low current | Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage |
|
| Wide and narrow bands in the gel | Differences in conductivity of the sample might be the cause. If the conductivity of the sample is high (more salt) the bands will become wide and if it is low (less salt) will become narrower. | Adding sample buffer to the blanks and ladder will help to equalize the conductivity and migration. |
