GenScript's Precast Gels

GenScript Precast Gel Selection Guide

GenScript's high quality mini gels generate the best electrophoresis performance with GenBox Mini Tank.

GenBox Mini Tank is designed for fast and consistent running of 2 mini gels at high voltages.

Other compatible gel tanks

Bio-Rad Mini-PROTEAN^® II & 3*

Bio-Rad Mini-PROTEAN^® Tetra System*

*Please reverse the gasket on the inner electrode assembly before use. See manual for detail.

Proteins are separated on SurePAGE™ using MOPS or MES running buffer. Tris-glycine buffer is NOT compatible with GenScript's precast gels. Choose the gel that best separates your protein of interest based on the migration chart below.

We recommend using lower concentration gels and MOPS buffer for large proteins (greater than 30kDa), higher concentration gels and MES buffer for small proteins (smaller than 30kDa).

Gel series	Well No.	Recommended Max. loading V./well	Well size	Recommended amount of protein/well
10x8 Gels	10	60 µl	80 µl	60 µg
	12	45 µl	60 µl	50 µg
	15	30 µl	40 µl	40 µg

For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.

Product Details

• SurePAGE™ Bis-Tris Gels (10x8)

  Free samples of SurePAGE™ (Including MOPS buffer powder) available. Contact us freesample@genscript.com.

  SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.

  Superior resolution

  SurePAGE
  (Bis-Tris)

  

  Competitor B
  (Tris-Glycine)

  

  Competitor T
  (Bis-Tris)

  

  Competitor T
  (Bis-Tris)

  

  ExpressPlus™
  (Bis-Tris)

  

  Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5 µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.

  Easier loading, Larger well volumes

  
• Gel Specifications

  Gel Specifications

  Gel Thickness	1.0 mm
  Gel Chemistry	Bis-Tris
  Gel Format	Mini Gels (10 x 8 cm)
  Storage and Shelf Life	18 months at 2-8℃; Or 6 months at room temperature (25℃) within 1 year after manufacturing date
  Running Buffer Recommendations	Tris-MOPS SDS (Cat. No. M00138) or MES SDS (Cat. No. M00677)

Product List

• SurePAGE™ ordering

  SurePAGE™ 10x8 gels ordering list

  Cat. No.	Product Name	Quantity	Price	Order
  M00652	SurePAGE™, Bis-Tris, 10x8, 4-12%, 10 wells		$89.00 10 PK/BOX
  M00653	SurePAGE™, Bis-Tris, 10x8, 4-12%, 12 wells		$89.00 10 PK/BOX
  M00654	SurePAGE™, Bis-Tris, 10x8, 4-12%, 15 wells		$89.00 10 PK/BOX
  M00655	SurePAGE™, Bis-Tris, 10x8, 4-20%, 10 wells		$89.00 10 PK/BOX
  M00656	SurePAGE™, Bis-Tris, 10x8, 4-20%, 12 wells		$89.00 10 PK/BOX
  M00657	SurePAGE™, Bis-Tris, 10x8, 4-20%, 15 wells		$89.00 10 PK/BOX
  M00658	SurePAGE™, Bis-Tris, 10x8, 8-16%, 10 wells		$89.00 10 PK/BOX
  M00659	SurePAGE™, Bis-Tris, 10x8, 8-16%, 12 wells		$89.00 10 PK/BOX
  M00660	SurePAGE™, Bis-Tris, 10x8, 8-16%, 15 wells		$89.00 10 PK/BOX
  M00661	SurePAGE™, Bis-Tris, 10x8, 8%, 10 wells		$89.00 10 PK/BOX
  M00662	SurePAGE™, Bis-Tris, 10x8, 8%, 12 wells		$89.00 10 PK/BOX
  M00663	SurePAGE™, Bis-Tris, 10x8, 8%, 15 wells		$89.00 10 PK/BOX
  M00664	SurePAGE™, Bis-Tris, 10x8, 10%, 10 wells		$89.00 10 PK/BOX
  M00665	SurePAGE™, Bis-Tris, 10x8, 10%, 12 wells		$89.00 10 PK/BOX
  M00666	SurePAGE™, Bis-Tris, 10x8, 10%, 15 wells		$89.00 10 PK/BOX
  M00667	SurePAGE™, Bis-Tris, 10x8, 12%, 10 wells		$89.00 10 PK/BOX
  M00668	SurePAGE™, Bis-Tris, 10x8, 12%, 12 wells		$89.00 10 PK/BOX
  M00669	SurePAGE™, Bis-Tris, 10x8, 12%, 15 wells		$89.00 10 PK/BOX

• ExpressPlus™ ordering

  ExpressPlus™ 10x8 gel ordering list

  Cat. No.	Product Name	Quantity	Price	Order
  M00810	ExpressPlus™ PAGE Gel,10×8, 8%, 10 wells		$147.00 20 Gels
  M00812	ExpressPlus™ PAGE Gel,10×8, 8%, 12 wells		$147.00 20 Gels
  M00815	ExpressPlus™ PAGE Gel,10×8, 8%, 15 wells		$147.00 20 Gels
  M01010	ExpressPlus™ PAGE Gel,10×8, 10%, 10 wells		$147.00 20 Gels
  M01012	ExpressPlus™ PAGE Gel,10×8, 10%, 12 wells		$147.00 20 Gels
  M01115	ExpressPlus™ PAGE Gel,10×8, 10%, 15 wells		$147.00 20 Gels
  M01210	ExpressPlus™ PAGE Gel,10×8, 12%, 10 wells		$147.00 20 Gels
  M01212	ExpressPlus™ PAGE Gel,10×8, 12%, 12 wells		$147.00 20 Gels
  M01215	ExpressPlus™ PAGE Gel,10×8, 12%, 15 wells		$147.00 20 Gels
  M41210	ExpressPlus™ PAGE Gel,10×8, 4-12%, 10 wells		$147.00 20 Gels
  M41212	ExpressPlus™ PAGE Gel,10×8, 4-12%, 12 wells		$147.00 20 Gels
  M41215	ExpressPlus™ PAGE Gel,10x8, 4-12%, 15 wells		$147.00 20 Gels
  M42010	ExpressPlus™ PAGE Gel,10×8, 4-20%, 10 wells		$147.00 20 Gels
  M42012	ExpressPlus™ PAGE Gel,10×8, 4-20%, 12 wells		$147.00 20 Gels
  M42015	ExpressPlus™ PAGE Gel,10×8, 4-20%, 15 wells		$147.00 20 Gels
  M81610	ExpressPlus™ PAGE Gel,10×8, 8-16%, 10 wells		$147.00 20 Gels
  M81612	ExpressPlus™ PAGE Gel,10×8, 8-16%, 12 wells		$147.00 20 Gels
  M81615	ExpressPlus™ PAGE Gel,10×8, 8-16%, 15 wells		$147.00 20 Gels

• Related products

  Cat. No.	Product Name	Quantity	Price	Order
  L00671	Tank Adaptor		$12.00 2 pcs/unit
  L00674	Cassette Opener		$10.00 1 kit
  L00699	Buffer Dam		$8.00 1 pc
  L00780	GenBox Mini Electrophoresis tank		$480.00 1 unit
  L00781	GenBox Mini Blot module		$500.00 1 unit
  M00138	Tris-MOPS-SDS Running Buffer Powder		$25.00 1 Box (5/PK)
  M00139	Transfer Buffer Powder		$58.00 1 Box (10/PK)
  M00624	Broad Multi Color Pre-Stained Protein Standard		$72.00 250 μl 1250 μl (250μl x 5)
  M00676	4X LDS Sample Buffer		$12.00 10 ml 250 ml
  M00677	MES SDS Running Buffer Powder		$25.00 1 box(5pcs)
  MB01015	5X Sample Buffer		$24.00 5 ml

Resources

FAQ

• 1. What's the difference between ExpressPlus™ and SurePAGE™ gels?

  SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency, and shorter running time compared to ExpressPlus™ gels.

• 2. How long does it take to run an ExpressPlus™ or SurePAGE™ gel?

  It depends on the gel type and concentration. SurePAGE™ gels can run at 200V for 20 minutes using MES running buffer or 30 minutes using MOPS running buffer. ExpressPlusTM gels run at 140V for 50 minutes.

• 3. What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?

  GenScript’s gels generate the best performance using GenBox Mini Gel tank and are also compatible with Bio-Rad Mini Gel Tanks (see manual for user instructions). Other gel tanks are not recommended.

• 4. Is this gel stable at room temperature?

  Yes. SurePAGE™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.

• 5. Which sample loading buffer should I choose?

  We recommend using 4xLDS sample buffer (Cat. No. M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.

• 6. What running buffer should I use to run SurePAGE™ gels?

  We recommend MOPS for large and medium proteins (greater than 30kDa), and MES for small proteins (smaller than 30kDa). Tris-glycine buffer is NOT compatible with GenScript's precast gels.

• 7. What's the buffer I should use to transfer the gel?

  We recommend Tris Bicine transfer buffer (M00139).

• 8. Will overnight destaining affect the resolution of the band?

  For overnight destaining, we suggest using a weaker destaining solution consisting of 15% methanol and 10% acetic acid in water.

• Trouble shooting

  Problems	Probable cause	Solution
  Distorted protein bands	Air bubbles in the sample wells	Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading
  Some part of the tracking dye changed to yellow	Buffer enters gel because of broken cassette	Gel tank is not compatible or cassette was damaged
  	Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage	Add more running buffer in the cathode tank cassette (between the two gels) until it overflows from the gel wells; Press the gel firmly into the cassette to ensure it is fully sealed; Use a functional/not damaged thank cassette
  Streaking	Insoluble or weakly charged particles (such as carbohydrates) exist in the sample	Heat up the sample in the presence of SDS, centrifuge sample and load the supernatant
  Electrophoresis time is too long	Check the specifications of the power supply if it can support multiple gel runs at the same time	Peel the seal off from the bottom of cassette before loading
  	Incorrect running conditions	Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis
  Electrophoresis does not start with error shown on the power supply	Seal is not removed from the bottom of the cassette	Peel the seal off from the bottom of cassette before loading
  Bands are not well separated	Incorrect gel percentage	Use the protein migration table to choose the appropriate gel
  	Sample overloading	Reduce sample loading amount, especially when the sample contains many kinds of protein.
  	Insufficient SDS in loading buffer	Increase SDS content during sample preparation
  	Insufficient buffer to keep tank cool	Add more buffers to the outer tank until it is at the same level or above the top of the sample wells
  Sample spreading across the gel	Sample contains too much salt	Reduce sample salt content by dialysis or ultra-filtration
  The voltage cannot reach the set value	Leaking between the inner and outer tank during run	Use compatible gel tank
  	Run too many gels on the same power supply	Check the specifications of the power supply if it can support multiple gel runs at the same time
  Lots of air bubbles between the gel and the cassette	Overheat might be the cause, especially if running buffer is hot after electrophoresis	Add more running buffer to the outer tank
  Sample do not separate or migrate with a low current	Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage	Add more running buffer in the cathode tank cassette (between the two gels) until it overflows from the gel wells; press the gel firmly into the cassette to ensure it is fully sealed; use a functional/not damaged tank cassette
  Wide and narrow bands in the gel	Differences in conductivity of the sample might be the cause. If the conductivity of the sample is high (more salt) the bands will become wide and if it is low (less salt) will become narrower.	Adding sample buffer to the blanks and ladder will help to equalize the conductivity and migration.

Technical Resources

Product Documents

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