High Resolution & Robust Separation
- Large well volume – Up to 80 µl for diluted samples
- High resolution – Even, sharp bands, guaranteed lot-lot consistency
- Long shelf life – Up to 12 months at 2-8℃
- Cost effective – 30-50% price reduction compared to other major competitors
- Compatible cassette design – Fits all popular mini-gel tanks
GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.
GenScript's gel series include-SurePAGE™ Gels which are the premium, higher resolution and reproducibility gels, and ExpressPlus, good quality and very cost-effective gels.
View the detailed comparison between SurePAGE,
ExpressPlus and other major gel types.
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ExpressPlus (Bis-Tris)
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SurePAGE (Bis-Tris)
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Competitor B (Tris-Glycine)
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Homemade (Tris-Glycine)
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Competitor T (Bis-Tris)
SurePAGE™ Bis-Tris Gels (10x8)
Free samples of SurePAGE™ (Including MOPS buffer powder) available. Contact us freesample@genscript.com.
SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.
Superior resolution
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SurePAGE
(Bis-Tris)
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Competitor B
(Tris-Glycine)
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Homemade
(Tris-Glycine)
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Competitor T
(Bis-Tris)
Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.
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Cost effective
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Easier loading, Larger well volumes
GenScript Precast gel selection guide
Compatible gel tanks
Bio-Rad Mini-PROTEAN® II & 3*
Bio-Rad Mini-PROTEAN®Tetra System*
Invitrogen Novex XCell I, II, & Surelock® (Must purchase Tank Adaptor, catalog number L00671)
LONZA PAGEr® Minigel Chamber
Hoefer Mighty Small (SE 260/SE 250)
Hoefer Tall Mighty Small (SE 280)
*please reverse the gasket, see instructions below
With MOPS running buffer
With MES running buffer
| Gel series | Well No. | Recommended Max. loading V./well | Well size | Recommended amount of protein/well |
|---|---|---|---|---|
| 10x8 Gels | 10 | 60µl | 80µl | 60µg |
| 12 | 45µl | 60µl | 50µg | |
| 15 | 30µl | 40µl | 40µg |
For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.
An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.
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| Buy 4 or more boxes of SurePAGE Bis-Tris Gels and get a FREE T-shirt. Call us at 732-885-9188 to place your order or email us at product@genscript.com. to obtain a quote. Hurry while supplies last! Offer valid only with a quote and applies to SurePAGE Bis-Tris Gels only.
Get a FREE T-shirt
*Offer valid for US and Canada customers only.
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| Resources |
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SurePAGE™ 10x8 gels ordering list
Order 10 boxes or more and save $10/box!
ExpressPlus™ 10x8 gel ordering list
Cat. No. |
Product Name |
Price |
|
|---|---|---|---|
L00671 |
Tank Adaptor - 2 pcs/unit |
$12.00 |
|
L00674 |
Cassette Opener - 1 kit |
$10.00 |
|
L00699 |
Buffer Dam - 1 pc |
$8.00 |
|
M00676-10 |
4X LDS Sample Buffer - 10 ml |
$10.00 |
|
M00676-250 |
4X LDS Sample Buffer - 250 ml |
$90.00 |
|
M00677 |
MES SDS Running Buffer Powder - 1 box(5pcs) |
$25.00 |
|
MB01015 |
5X Sample Buffer - 5 ml |
$24.00 |
|
M00138 |
Tris-MOPS-SDS Running Buffer Powder - 1 Box (5/PK) |
$25.00 |
|
M00139 |
Transfer Buffer Powder - 1 Box (10/PK) |
$58.00 |
|
M00624-250 |
Broad Multi Color Pre-Stained Protein Standard - 250 µl |
$70.00 |
|
M00624-1250 |
Broad Multi Color Pre-Stained Protein Standard - 1250 µl (250µl x 5) |
$280.00 |
*Tank Adaptor(Cat. No. L00671) is for use with Invitrogen Novex XCell I, II, & Surelock®
FAQ
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What's the difference between ExpressPlus™ and SurePAGE™ gels?
SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency compared to ExpressPlus™ gels.
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How long does it take to run a ExpressPlus™ or SurePAGE™ gel?
It depends on the concentration of the gel. Usually it takes about 50 minutes at 140V. Shorter running times can be achieved if a higher voltage is used, but higher than 180V is not recommended.
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What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?
Any tank that is designed to run 10cm x 8cm (length x width) precast gels. See product page for the list.
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Is this gel stable at room temperature?
Yes. SurePAGE™ and ExpressPlus™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.
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Which sample loading buffer should I choose?
We recommend using 4xLDS sample buffer (M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.
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What running buffer should I use to run the gel?
We recommend MOPS for large and medium proteins, and MES for small proteins. Tris-glycine buffer is NOT compatible with GenScript's precast gels.
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What's the buffer I should use to transfer the gel?
We recommend Tris Bicine transfer buffer (M00139).
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Why do some parts of the gel become sticky following protein transfer?
This is caused by the concentrated polyacrylamide on the top part of the gel. It will help if this part of the gel is cut and removed before transfer.
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Will overnight destaining affect the resolution of the band?
It depends. We suggest using a destaining solution consisting of 15% methanol and 10% acetic acid in DI water for overnight destaining.
Trouble shooting
| Problems | Probable cause | Solution |
|---|---|---|
| Distorted protein bands | Air bubbles in the sample wells | Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading |
| Some part of the tracking dye changed to yellow | Buffer enters gel because of broken cassette | Gel tank is not compatible or cassette was damaged |
| pH value decreased | Prepare new running buffer with deionized water. Check pH | |
| Streaking | Insoluble or weakly charged particles (such as carbohydrates) in sample | Heat sample in the presence of SDS, centrifuge sample and load the supernatant |
| Electrophoresis time is too long | Seal is not removed from the bottom of the cassette | Peel the seal off from the bottom of cassette before loading |
| Incorrect running conditions | Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis | |
| Bands are not well separated | Incorrect gel percentage | Use the protein migration table to choose the appropriate gel |
| Sample overloading | Reduce sample loading amount, especially when the sample contains many kinds of protein. | |
| Insufficient SDS in loading buffer | Increase SDS content in the sample during preparation | |
| Insufficient buffer to keep tank cool | Add more buffers to the outer tank until it's at the same level or above the top of the sample wells | |
| Sample spreading across the gel | Sample contains too much salt | Reduce salt content by dialysis or ultra-filtration |
| The voltage cannot reach the set value | Leaking between the inner and outer tank during run | Use compatible gel tank |
| Excess salt in the sample | Reduce salt content by dialysis or ultra-filtration | |
| Lots of air bubbles between the gel and the cassette | Running buffer is hot after electrophoresis | Add more running buffer to the outer tank |
