Routine PCR Reagents

A: .5 U or more

A: The Green Taq contains Taq polymerase with a special stabilizer that allows it to be stable at room temperature for 2 weeks.

A: No.

A: The Taq DNA polymerase is 100% active in the buffer supplied by GenScript, but also works in competitors' buffers. For your convenience, we sell 10 x Taq buffer Cat. No. B0005

A: We suggest using Mg-free Taq DNA Polymerase (Cat. No. E00008).

A: Here are the guidelines for 50 μl PCR reaction:

• 0.5 μl Taq DNA Polymerase
• 1 μl 10 mM dNTP
• 1 μl each primer
• 2 μl genomic template (up to 100 ng/μl)
• 5 μl 10 X Taq Buffer (Standard Taq containing Mg²⁺)
• 39.5 μl sterile or filtered H₂O
• Denature at 94°C
• Extend 1 minute/kb

A:

• For up to 3 kb, we recommend 1.25 units per 50 µl reaction


• For specialized applications, including 3–8 kb amplicons, 2.5 units per 50 µl reaction is recommended

A: Up to λdna 8 kb.

A: The Poly A tail contains sticky ends, and can only do TA cloning

A: There may be a problem in the PCR program or system, or a problem during the electrophoresis.

A: We suggest to use a high-fidelity DNA Polymerase which can offer the highest fidelity of any commercial thermostable polymerase.

A: The Taq can be used when the PCR reaction started or after degeneration

A: No. The exonuclease will only degrade double stranded DNA that it encounters while extending a DNA fragment. It will degrade a secondary primer if bound to the same strand (e.g. a mutagenesis primer).

A: Please send all your issue via product@genscript.com, or load the protocol of the Taq DNA polymerase direcly.