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Peptide purity, usually determined by reversed-phase HPLC, refers to the percentage of the target peptide compared to impurities that absorb at 220 nm (peptide bond absorption wavelength). Impurities are usually deletion peptide sequences generated during peptide synthesis. Peptide purity will affect experimental results since the lot-to-lot variability becomes even greater the lower the peptide purity is (especially <80% purity).
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90%+ scientists prefer peptides over >75% purity
GenScript offers reliable peptides with purities ranging from crude to as high as >98%, at 100% guaranteed quantity with fast delivery to support your peptide-related projects.
An appropriate peptide purity is a critical factor for the success of a scientific experiment. In general, the higher the peptide purity level, the more favorable the experimental outcome. Impurities in lower purity peptides may result in toxicity or unwanted side reactions, especially for some applications such as animal studies where exceedingly pure peptides (>98% purity) are required. Considering budget, there are minimum acceptable purity levels recommended for various applications as follows:
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