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Plasmid DNA Preparation Services Upgraded
High-quality Plasmid DNA Supports Cloning, Transfection, And
Animal Study
Service Details
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Tier 1
Plasmid DNA
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Tier 2
Plasmid DNA
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Tier 3
Plasmid DNA |
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Primary Application |
Cloning |
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Transfection |
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Animal Study |
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Quantity Range |
10µg – 1mg |
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10µg – 2g+ |
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10mg – 2g+ |
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Homogeneity |
Predominantly supercoil |
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≥80% supercoil |
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≥90% supercoil |
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Endotoxin Level |
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- ≤ 0.1 EU/μg
- ≤0.01 EU/μg
- ≤0.005 EU/μg
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Benefits |
- The most stringent default quality control
- Flexible optional quality control test for your need
- Multiple endotoxin levels up to your choice
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Deliverables Include:
- µg to g amounts of plasmid according to your request
- Quality assurance certificate
Please Note:
- Get an instant quote and estimated turnaround time for your gene synthesis bundled plasmid DNA
preparation via GenSmart™ 2.0 online ordering platform.
- The original Research Grade plasmid DNA combines "Predominantly supercoil" with "Not
guarantee"
endotoxin level. The original Industrial Grade plasmid DNA combines "≥80%" supercoil
level with "≤0.01
EU/µg" endotoxin level. And the original Pre-clinical Grade plasmid DNA combines "≥90%" supercoil level
with "≤0.01 EU/µg" endotoxin level.
Quality Control Strategies
GenScript's plasmid DNA preparation services provide unparalleled quality control standards in the market,
offering customizable solutions tailored to meet your specific requirements for both quality and quantity.
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Default and Optional Quality Control Tests
| QC Test |
Specification |
Tier 1 Plasmid DNA
(Predominantly supercoil)
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Tier 2 Plasmid DNA
(≥80% supercoil)
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Tier 3 Plasmid DNA
(≥90% supercoil)
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| Homogeneity1, 5
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Depends on your choice |
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| Endotoxin Level
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Depends on your choice |
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| Quantity
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Depends on your choice |
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| Sequence Accuracy2
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100% alignment with reference sequence |
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| Appearance
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Colorless, clear, free of precipitate or foreign particles |
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| A260/280
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1.8-2.0 |
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| Restriction Analysis
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DNA bands with expected sizes detected by agarose gel electrophoresis |
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| Residual RNA
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Not visible on agarose gel |
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| E.coli Genomic DNA3, 5
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< 15% by agarose gel electrophoresis |
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| ≤ 5% by qPCR |
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| Residual Host Protein5
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≤ 1% |
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| Bio-Burden Test
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No viable microorganisms in /on culture media |
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| pH
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8.0±0.5 (in TE buffer) |
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| Mycoplasma Contamination
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Negative |
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| Phage Detection
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No Phage plaques |
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Optional Production Processes
| Process |
Specification |
Tier 1 Plasmid DNA
(Predominantly supercoil)
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Tier 2 Plasmid DNA
(≥80% supercoil)
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Tier 3 Plasmid DNA
(≥90% supercoil)
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| Animal-free Production
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Provide TSE/BSE statement |
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| Sterile Filtration
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Pass Bio-Burden test |
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| Documentation4
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Traceable data records |
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| Upgraded Purification5
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Upgraded QC standards for supercoil, residual host protein, residual DNA |
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| AAV ITR Guarantee
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Accurate AVV ITR structure |
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| Poly A Guarantee
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Complete poly (A) tail |
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Please Note:
- The percentage of supercoiled plasmid DNA may vary when analyzed with different methods or can be
altered due to shipping conditions.
- Sequence verification of the full gene insert only applies when bundled with gene synthesis. The whole
plasmid sequencing by NGS or nanopore is available with an additional charge.
- For tier 1 and tier 2 plasmid DNA, the E. coli genomic DNA < 15% by agarose gel electrophoresis refers
to the intensity of genomic DNA band is less than 15% of that of all bands on the gel. For tier 3
plasmid DNA, the E. coli genomic DNA is less than 5% and verified by qPCR method.
- Documentation is only available to request offline, please contact your account manager or email to
[email protected] to order or get a quote.
- By adding the purification upgrade add-on service, the ≥90% supercoiled plasmid DNA is verified by HPLC,
and the E. coli genomic DNA is ≤ 1%, as confirmed by qPCR (for high copy plasmids only). Residual host
protein is ≤ 0.1% and verified by ELISA.
GenSmart™ 2.0 Online Ordering Platform beta-testing
Sequence
input
Clone Setup &
Codon optimization
Plasmid Setup &
Add-ons
Order or
Get a Quote
Order gene synthesis bundled plasmid DNA
preparation via GenSmart™
2.0
- Utilize the best-in-class codon optimization algorithm to optimize your sequence
- Select the most suitable gene synthesis, cloning, and plasmid DNA preparation services for
your applications
- Receive estimated pricing and turnaround time
- Get an instant quote and place your order
Global Manufacturing Network
Cutting-edge facilities in China, the United States, and Singapore offer top-quality gene
synthesis, cloning, and plasmid DNA services to global researchers. Our worldwide presence ensures quick and
efficient access to our top-notch services, enabling researchers to focus on their core work with
confidence. Trust in our expertise and global reach to support your research needs and propel innovation in
the field of genetic sciences.
Get in Touch
with GenScript Experts
