High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently
coupled to a chelating agent that binds Ni2+ by four coordination
sites for high-affinity purification of polyhistidine-tagged recombinant
proteins. High Affinity Ni-Charged Resin has
low Ni2+ leakage, high protein-binding capacity and stability, and
is compatible with a wide range of additives used in protein purification. This
makes High Affinity Ni-Charged Resin the excellent choice for high performance
purification of polyhistidine-tagged proteins.
High temperature heating is not recommended. The agarose melts above 65°C.
Applications:1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.
Characteristics of High Affinity Ni-Charged Resin
Reagents Compatible with High Affinity Ni-Charged Resin
|Storage||2-8 °C; DO NOT FREEZE|
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