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Catalog Products » Other Products » Monofinity A Resin

Monofinity A Resin

Protein A affinity chromatography resin is the most commonly applied method for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus. It has five high affinity binding sites capable of binding specifically to the Fc region of immunoglobulin molecules from several species. Covalently immobilized Protein A matrices have been extensively used to purify IgG from several species of mammals.

GenScript's Monofinity A Resin is made of recombinant protein A as ligand, which not only keeps the specific binding capacity to Fc region of immunoglobulin molecules, but also enhances chemical stability under Cleaning-in-place (CIP) conditions. The typical dynamic binding capacity is more than 30 g human IgG/L settled resin. There is no significant decrease of binding capacity after purification/CIP for 200 cycles. In the meanwhile, the Monofinity A Resin has a low leakage of ligand (< 15 ng ligand/mg IgG) over a number of purification/CIP cycles.

As a high quality antibody purification resin, it is highly suitable for large scale antibody purification, and meets the purification requirements of industry-scale customers.

Product Details

Cat.No. Product Name Volume supplied Resin content
L00433-55 Monofinity A Resin Prepacked Column (5mlx5) 5 ml x 5 Prepacked column
L00433-51 Monofinity A Resin Prepacked Column (5mlx1) 5 ml x 1
L00433-11 Monofinity A Resin Prepacked Column (1mlx1) 1 ml x 1
L00433-15 Monofinity A Resin Prepacked Column (1mlx5) 1 ml x 5
L00433-25 Monofinity A Resin (25ml) 50 ml 50% slurry
L00433-200 Monofinity A Resin (200ml) 400 ml 50% slurry
L00433-1 Monofinity A Resin (1L) 2 L 50% slurry
L00433-5 Monofinity A Resin (5L) 10 L 50% slurry
L00433-10 Monofinity A Resin (10L) 20L 50% slurry

  • Laboratory scale antibody purification
  • Bioprocess scale antibody purification

Store in binding/ washing buffer containing 20% ethanol at 2-8°C.Do NOT freeze
Composition Highly cross-linked 4% agarose
Average particle size 90 μm
Ligand Alkali-tolerant recombinant protein A (E. coli)
Dynamic binding capacity (DBC) >30mg human lgG/ ml
Ligand leakage <15ng/ mg
Recommended flow rate 50 to 300 cm/ h
Chemical stability Stable in all commonly used buffers
pH working range 3-12
CIP stability 0.1-0.5M NaOH

Key Features
Key Features
  • High dynamic binding capacity: >30 mg IgG/ml settled resin
  • High recovery rate: >97% in average
  • Enhanced stability: good thermal and chemical stability
  • Low ligand leakage: < 15 ng/mg IgG
  • Reliable scale-up: highly suitable for large scale antibody purification
  • Alkaline tolerance: Clean in place with 0.1 M NaOH

  • Store in binding/ washing buffer containing 20% ethanol at 2-8°C

  • Do NOT freeze

    For more documents, please visit "Technical Support".

    Cat. No. L00433
    Size Price
    10 L Resin (Total 20 L) Quote
    5 L Resin (Total 10 L) Quote
    1 L Resin (Total 2 L) Quote
    25 ml Resin (Total 50 ml) $1680.00
    200 ml Resin (Total 400 ml) $3350.00