GenScript’s AmMag Nimagnetic beads are designed for purification and screening of histidine-tagged (his-tagged) proteins. The beads are highly EDTA and DTT resistant, enabling them to bind proteins in the presence of chelators.The beads are also optimized for easy clean up and regeneration, for reuse with multiple samples.
|Ligand||Nickel-charged TED (tris-carboxymethyl ethylene diamine)|
|Binding capacity||About 10 mg histidine-tagged protein/ml settled beads|
|Matrix||Highly cross-linked spherical 4% agarose including magnetite|
|Average particle size||~70μm|
|Storage solution||20% ethanol (25% slurry)|
|Storage||2-8 °C (Do NOT freeze)|
Quick and convenient separation accomplished by magnetic force.
High binding capacity with 10mg of His-tagged protein/ml settled beads.
The AmMag Ni magnetic beads can be regenerated and reused up to 100 times in absence of chelators.
Extremely low nonspecific binding.
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