CytoSinct™ CD19 Nanobeads, human

The CytoSinct CD19 Nanobeads, human, are used for in vitro enrichment or depletion of CD19+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD19.

Price	$180.00
Cat. No.	L00957-0.5
Size	500 μl 1 ml Buy in bulk
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Overview

Product Description

The CytoSinct CD19 Nanobeads, human is used for in vitro enrichment or depletion of CD19⁺ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD19.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct Column placed in the CytoSinct Magnet, or other compatible columns with magnets.
The CD19⁺ cells labeled with Nanobeads are retained within the column and unlabeled cells (CD19^- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD19⁺ cells can be eluted as the positively selected cell fraction.

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Properties

Capacity

Cat. No.	Product Name	Size	Capacity
L00957-0.5	CytoSinct™ CD19 Nanobeads, human	500 μL	Up to 5 × 10⁸ total cells
L00957-1	CytoSinct™ CD19 Nanobeads, human	1 mL	Up to 1 × 10⁹ total cells

Format

Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.

Components

1 mL or 0.5 mL CytoSinct CD19 Nanobeads, human
Nanobeads conjugated to monoclonal anti-human CD19 antibodies (isotype: chimeric IgG1).

Storage

Store at 2 - 8 °C. Do not freeze.

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CD19⁺ B cells were enriched or depleted from human PBMCs. CD19⁺ cells were stained with antiCD19 (clone: 4G7) PE and gated on Live/hCD45⁺. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.